eDNA metabarcoding data of a long-distance Southern Ocean transect - RV Aurora Australis, V1 2019

• Other Authors: AADC (owner), AADC, DATA OFFICER (distributor), AADC, DATA OFFICER (custodian), AU/AADC > Australian Antarctic Data Centre, Australia (hasAssociationWith), Australian Antarctic Data Centre (publisher), Australian Antarctic Division (sponsor), DEAGLE, BRUCE (hasPrincipalInvestigator), NESTER, GEORGIA (hasPrincipalInvestigator), Nester, G., Suter, L., Deagle, B., Polanowski, A., Kitchener, J., Bunce, M. and Wasserman, J. (originator), POLANOWSKI, ANDREA (hasPrincipalInvestigator), SUTER, LEONIE (hasPrincipalInvestigator)
• Format: Dataset
• Language: unknown
• Published: Australian Ocean Data Network
• Subjects: oceans; biota; EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > BIOMASS DYNAMICS; BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS > EUPHAUSIIDS (KRILL); ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON; BIODIVERSITY; BIOSECURITY; BIOMONITORING; ENVIRONMENTAL DNA; METABARCODING; PCR; MISEQ; SEAWATER INTAKE LINE; ADS > Automated DNA Sequencer; R/V AA > R/V Aurora Australis; AMD; AMD/AU; CEOS; OCEAN > SOUTHERN OCEAN; CONTINENT > ANTARCTICA; GEOGRAPHIC REGION > POLAR; Antarc*; Antarctica; aurora australis; Southern Ocean
• Online Access: https://researchdata.edu.au/edna-metabarcoding-long-v1-2019/3340505

Progress Code: completed These data accompany the paper 'Long-distance Southern Ocean environmental DNA (eDNA) transect provides insights into spatial marine biota and invasion pathways for non-native species'. Samples (n=138) were collected aboard the RSV Aurora Australis on a resupply voyage between Hobart, Tasmania (42°52’54.84”S 147°20’29.76”E) and Davis Station, Antarctica (66°26’14.28”S 77°28’24.6”E) in November 2019 (Figure 1; Table A1). Two combinations of water volume and filter pore sizes were tested at each sampling location: 12 L with 20 μm, and 2 L with 0.45 μm, herein referred to as “LargeVF” and “SmallVF” respectively. Water samples were collected and filtered approximately every 4 hours (4.52 ± 0.23) via the ship’s uncontaminated seawater intake line (4 ± 2 m depth). The seawater intake line was run for 3 – 5 minutes prior to sample collection to ensure the sample collected represented the seawater surrounding the ship at the time. SmallVF water samples (2 L, n=69) were filtered using a Sentino microbiology peristaltic pump (Pall Life Sciences) through 47 mm diameter, 0.45 μm pore size polyethersulfone filter membranes (Pall Life Sciences). Simultaneously, LargeVF water samples (12 L, n=69) were filtered using a Masterflex L/S console pump system (Cole-Parmer) through 25 mm diameter, 20 μm pore size nylon filter membranes (Merck). SmallVF filter membranes (47 mm diameter) were cut in half and immediately preserved at -80°C, with one half to be analysed and one to be stored as a reserve and a form of eDNA biobanking (Jarman et al., 2018). LargeVF filter membranes were not cut in half due to their smaller diameter (25 mm) and were stored whole at -80°C. Filtration equipment was rinsed with a 10% bleach solution and freshwater from the laboratory in between every sample, and soaked for 15 minutes with 10% bleach every tenth sample. Field controls consisted of 500 mL samples (n=10) of laboratory freshwater and the 10% bleach solution used for sterilisation, taken approximately every 10 samples. DNA ...