Antifreeze molecules in Nototheniid fish around Davis Station, Antarctica

• Other Authors: AADC (owner), AADC, DATA OFFICER (distributor), AADC, DATA OFFICER (custodian), AU/AADC > Australian Antarctic Data Centre, Australia (hasAssociationWith), Australian Antarctic Data Centre (publisher), Australian Antarctic Division (sponsor), CONNELL, DAVE J. (author), HAYMET, TONY (hasPrincipalInvestigator), WILLIAMS, DICK (collaborator), WILLIAMS, DICK (hasPrincipalInvestigator)
• Format: Dataset
• Language: unknown
• Published: Australian Ocean Data Network
• Subjects: biota; oceans; EARTH SCIENCE > OCEANS > SEA ICE > ICE DEPTH/THICKNESS; PACK ICE; SEA ICE; BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > FISH; BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > COASTAL; PELAGIC; MARINE ECOSYSTEMS; Nototheniid fish; antifreeze; fishing; FIELD SURVEYS; SHIPS; LABORATORY; FIELD INVESTIGATION; AMD/AU; CEOS; AMD; CONTINENT > ANTARCTICA > Davis; GEOGRAPHIC REGION > POLAR; Antarc*; Antarctica; Sea ice
• Online Access: https://researchdata.edu.au/antifreeze-molecules-nototheniid-station-antarctica/2822328

Progress Code: completed Statement: During the summer season, fishing was carried out on a regular basis (several days per week) principally from inflatable rubber boats (IRBs) using rods, handlines and/or fish traps. Fishing was also undertaken from the shoreline. Fishing from the shore proved to be most successful when traps were deployed. Casting from the shore, more often than not, resulted in lines becoming snagged on rocks and macroalgae. During winter, nearshore fishing sites were accessed by travel on foot or by skis. Traps were deployed through tide cracks or drilled holes. Fishing holes were drilled with a manual ice corer or a petrol powered jiffy drill. Fishtraps were used as the main means of collecting fish during winter as it was often too cold to sit outside for long periods with a rod and line. Blood samples were obtained from the caudal vein of the fish collected. Samples were taken in the field where possible. Blood samples were then transferred from the syringe to an eppendorf tube and stored overnight in a refrigerator to stimulate the red blood cells to settle out and clot. On the following day, tubes were transferred to a centrifuge and spun at 10,000 revolutions/sec for 4 to 5 minutes. The serum was then removed, transferred to a cryovial and stored in a -84 degrees C freezer. Muscle tissue samples were also taken. Small samples were removed from the anterior section of the tail. Tissue was excised between the lateral lines of the fish. Tissue was then transferred to a cryovial and stored in a -84 degrees C freezer. Both blood sera and muscle tissue samples were later transferred to a dewar of liquid nitrogen for transport back to Australia. Body mass, total body length and measures of snout-vent length were also obtained as were the location and depth of the fished site. All fish were then preserved in 10% formalin for a period of approximately 2 weeks and later transferred to 70% ethanol. A report completed as part of this project is available for download from the URL given below. ...