Winter diet of Gentoo penguins in South Georgia
Progress Code: completed Purpose This study uses tracking data and DNA analysis of scats during the 2018 winter to evaluate spatial and dietary overlap of gentoo penguins with the krill fishery. See spreadsheets - Gentoo Experiment Details 18s Each number corresponds to each worksheet 1. Samples and...
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Online Access: | https://researchdata.edu.au/winter-diet-gentoo-south-georgia/2822058 |
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ftands:oai:ands.org.au::2822058 2023-10-09T21:51:41+02:00 Winter diet of Gentoo penguins in South Georgia Spatial: westlimit=-38.06763; southlimit=-54.93977; eastlimit=-35.72754; northlimit=-53.94962 Temporal: From 2019-04-03 to 2019-09-19 https://researchdata.edu.au/winter-diet-gentoo-south-georgia/2822058 unknown Australian Ocean Data Network https://researchdata.edu.au/winter-diet-gentoo-south-georgia/2822058 BAS_Gentoo_Diet Dataset DOI AU/AADC > Australian Antarctic Data Centre, Australia biota oceans EARTH SCIENCE > OCEANS > AQUATIC SCIENCES > FISHERIES BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > SPECIES COMPETITION USE/FEEDING HABITATS BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > BIRDS > PENGUINS DNA DIET ANALYSIS GENTOO PENGUIN SSTI > Satellite-to-Satellite Tracking Instrument ADS > Automated DNA Sequencer LABORATORY FIELD INVESTIGATION AMD AMD/AU CEOS OCEAN > ATLANTIC OCEAN > SOUTH ATLANTIC OCEAN > SOUTH GEORGIA ISLAND > CUMBERLAND BAY GEOGRAPHIC REGION > POLAR OCEAN HARBOUR BARFF PENINSULA dataset ftands 2023-09-18T22:49:10Z Progress Code: completed Purpose This study uses tracking data and DNA analysis of scats during the 2018 winter to evaluate spatial and dietary overlap of gentoo penguins with the krill fishery. See spreadsheets - Gentoo Experiment Details 18s Each number corresponds to each worksheet 1. Samples and Date Gentoo penguin scats were collected from Cumberland Bay, South Georgia (from the Maiviken colony). Visits were made weekly between 3 April and 19 Sep 2018 (one visit in June was missed owing to avalanche risk). During each of the 24 visits, 25 fresh scats were collected, producing a total of 600 samples. Samples were scooped into a 2 ml plastic screw-top tube containing 80% ethanol with a clean spatula and frozen at -20 degrees. DNA was extracted from ~30 mg of faecal material using the Promega Maxwell RSC Tissue DNA Kit. Each extraction contained a soft part or ~500ml of EtOH slurry. The samples were spun down, the EtOH was poured off, the sample was re-suspended in 120ul of S.T.A.R buffer and homogenised. 100ul of the supernatant was added to well number 1 and samples were eluted in 100ul of TE. 2. Plate Layout Samples were diluted 1/10 and plated out on 96 well plates. Each plate had a positive control (fish, squid, shrimp DNA mix) and a negative PCR control. 3. 1st Round PCR. All samples were analysed using a highly conserved metazoan primer set that amplifies a region of the nuclear 18S gene ( McInnes et al. 2017a). The first round PCR is to amplify the target marker and add sample-specific (7bp) multiplex-identifier (MID) tags (forward and reverse primer) and Illumina sequencing primers. See sheet for PCR conditions. 4. 2nd Round PCR The second round PCR is to add sequencing adapters and additional 8 bp MIDs. See sheet for PCR conditions. 5. Miseq MiSeq genome sequencer (Illumina), using the MISEQ V2 reagent kits (300 cycles). See sheet for sample layout, i5 and i7 adapters and first round MID tags ########################################################################################### See ... Dataset Gentoo penguin South Atlantic Ocean South Georgia Island Research Data Australia (Australian National Data Service - ANDS) South Georgia Island ENVELOPE(-36.750,-36.750,-54.250,-54.250) Cumberland Bay ENVELOPE(69.052,69.052,-48.781,-48.781) Maiviken ENVELOPE(-36.508,-36.508,-54.239,-54.239) Ocean Harbour ENVELOPE(-36.247,-36.247,-54.334,-54.334) Barff Peninsula ENVELOPE(-36.314,-36.314,-54.313,-54.313) |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
biota oceans EARTH SCIENCE > OCEANS > AQUATIC SCIENCES > FISHERIES BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > SPECIES COMPETITION USE/FEEDING HABITATS BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > BIRDS > PENGUINS DNA DIET ANALYSIS GENTOO PENGUIN SSTI > Satellite-to-Satellite Tracking Instrument ADS > Automated DNA Sequencer LABORATORY FIELD INVESTIGATION AMD AMD/AU CEOS OCEAN > ATLANTIC OCEAN > SOUTH ATLANTIC OCEAN > SOUTH GEORGIA ISLAND > CUMBERLAND BAY GEOGRAPHIC REGION > POLAR OCEAN HARBOUR BARFF PENINSULA |
spellingShingle |
biota oceans EARTH SCIENCE > OCEANS > AQUATIC SCIENCES > FISHERIES BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > SPECIES COMPETITION USE/FEEDING HABITATS BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > BIRDS > PENGUINS DNA DIET ANALYSIS GENTOO PENGUIN SSTI > Satellite-to-Satellite Tracking Instrument ADS > Automated DNA Sequencer LABORATORY FIELD INVESTIGATION AMD AMD/AU CEOS OCEAN > ATLANTIC OCEAN > SOUTH ATLANTIC OCEAN > SOUTH GEORGIA ISLAND > CUMBERLAND BAY GEOGRAPHIC REGION > POLAR OCEAN HARBOUR BARFF PENINSULA Winter diet of Gentoo penguins in South Georgia |
topic_facet |
biota oceans EARTH SCIENCE > OCEANS > AQUATIC SCIENCES > FISHERIES BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > SPECIES COMPETITION USE/FEEDING HABITATS BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > BIRDS > PENGUINS DNA DIET ANALYSIS GENTOO PENGUIN SSTI > Satellite-to-Satellite Tracking Instrument ADS > Automated DNA Sequencer LABORATORY FIELD INVESTIGATION AMD AMD/AU CEOS OCEAN > ATLANTIC OCEAN > SOUTH ATLANTIC OCEAN > SOUTH GEORGIA ISLAND > CUMBERLAND BAY GEOGRAPHIC REGION > POLAR OCEAN HARBOUR BARFF PENINSULA |
description |
Progress Code: completed Purpose This study uses tracking data and DNA analysis of scats during the 2018 winter to evaluate spatial and dietary overlap of gentoo penguins with the krill fishery. See spreadsheets - Gentoo Experiment Details 18s Each number corresponds to each worksheet 1. Samples and Date Gentoo penguin scats were collected from Cumberland Bay, South Georgia (from the Maiviken colony). Visits were made weekly between 3 April and 19 Sep 2018 (one visit in June was missed owing to avalanche risk). During each of the 24 visits, 25 fresh scats were collected, producing a total of 600 samples. Samples were scooped into a 2 ml plastic screw-top tube containing 80% ethanol with a clean spatula and frozen at -20 degrees. DNA was extracted from ~30 mg of faecal material using the Promega Maxwell RSC Tissue DNA Kit. Each extraction contained a soft part or ~500ml of EtOH slurry. The samples were spun down, the EtOH was poured off, the sample was re-suspended in 120ul of S.T.A.R buffer and homogenised. 100ul of the supernatant was added to well number 1 and samples were eluted in 100ul of TE. 2. Plate Layout Samples were diluted 1/10 and plated out on 96 well plates. Each plate had a positive control (fish, squid, shrimp DNA mix) and a negative PCR control. 3. 1st Round PCR. All samples were analysed using a highly conserved metazoan primer set that amplifies a region of the nuclear 18S gene ( McInnes et al. 2017a). The first round PCR is to amplify the target marker and add sample-specific (7bp) multiplex-identifier (MID) tags (forward and reverse primer) and Illumina sequencing primers. See sheet for PCR conditions. 4. 2nd Round PCR The second round PCR is to add sequencing adapters and additional 8 bp MIDs. See sheet for PCR conditions. 5. Miseq MiSeq genome sequencer (Illumina), using the MISEQ V2 reagent kits (300 cycles). See sheet for sample layout, i5 and i7 adapters and first round MID tags ########################################################################################### See ... |
format |
Dataset |
title |
Winter diet of Gentoo penguins in South Georgia |
title_short |
Winter diet of Gentoo penguins in South Georgia |
title_full |
Winter diet of Gentoo penguins in South Georgia |
title_fullStr |
Winter diet of Gentoo penguins in South Georgia |
title_full_unstemmed |
Winter diet of Gentoo penguins in South Georgia |
title_sort |
winter diet of gentoo penguins in south georgia |
publisher |
Australian Ocean Data Network |
url |
https://researchdata.edu.au/winter-diet-gentoo-south-georgia/2822058 |
op_coverage |
Spatial: westlimit=-38.06763; southlimit=-54.93977; eastlimit=-35.72754; northlimit=-53.94962 Temporal: From 2019-04-03 to 2019-09-19 |
long_lat |
ENVELOPE(-36.750,-36.750,-54.250,-54.250) ENVELOPE(69.052,69.052,-48.781,-48.781) ENVELOPE(-36.508,-36.508,-54.239,-54.239) ENVELOPE(-36.247,-36.247,-54.334,-54.334) ENVELOPE(-36.314,-36.314,-54.313,-54.313) |
geographic |
South Georgia Island Cumberland Bay Maiviken Ocean Harbour Barff Peninsula |
geographic_facet |
South Georgia Island Cumberland Bay Maiviken Ocean Harbour Barff Peninsula |
genre |
Gentoo penguin South Atlantic Ocean South Georgia Island |
genre_facet |
Gentoo penguin South Atlantic Ocean South Georgia Island |
op_source |
AU/AADC > Australian Antarctic Data Centre, Australia |
op_relation |
https://researchdata.edu.au/winter-diet-gentoo-south-georgia/2822058 BAS_Gentoo_Diet Dataset DOI |
_version_ |
1779314815910543360 |