Southern Ocean eDNA metabarcoding raw sequencing data, collected on the Aurora Australis 2019-2020

• Other Authors: AADC (owner), AADC, DATA OFFICER (distributor), AADC, DATA OFFICER (custodian), AU/AADC > Australian Antarctic Data Centre, Australia (hasAssociationWith), Australian Antarctic Data Centre (publisher), Australian Antarctic Division (sponsor), DEAGLE, BRUCE (hasPrincipalInvestigator), MACDONALD, ANNA (hasPrincipalInvestigator), POLANOWSKI, ANDREA (hasPrincipalInvestigator), SUTER, LEONIE (collaborator), SUTER, LEONIE (hasPrincipalInvestigator), SUTER, LEONIE (author)
• Format: Dataset
• Language: unknown
• Published: Australian Ocean Data Network
• Subjects: biota; oceans; EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > FISH; ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS > EUPHAUSIIDS (KRILL); MAMMALS > CETACEANS; PCR; MISEQ; ENVIRONMENTAL DNA; METABARCODING; GENETIC SURVEY; ADS > Automated DNA Sequencer; R/V AA > R/V Aurora Australis; LABORATORY; AMD/AU; AMD; CEOS; OCEAN > SOUTHERN OCEAN; CONTINENT > ANTARCTICA; GEOGRAPHIC REGION > POLAR; Antarc*; Antarctic; Antarctica; aurora australis; Australian Antarctic Division; Southern Ocean
• Online Access: https://researchdata.edu.au/southern-ocean-edna-2019-2020/2821815

Progress Code: onGoing Purpose The purpose of this work is to determine best eDNA metabarcoding approaches to survey the Southern Ocean (SO). This includes comparisons of sampling strategy (2 L / 0.45 μm pore size filter vs 12 L / 20 μm pore size filter), PCR methodology (two step, dual MID tags vs...