Molecular comparison of bacterial diversity in uncontaminated and hydrocarbon contaminated marine sediment

• Other Authors: AADC (owner), AADC, DATA OFFICER (distributor), AADC, DATA OFFICER (custodian), AU/AADC > Australian Antarctic Data Centre, Australia (hasAssociationWith), Australian Antarctic Data Centre (publisher), Australian Antarctic Division (sponsor), BOWMAN, JOHN (hasPrincipalInvestigator), Bowman, J. and Powell, S. (originator), POWELL, SHANE (collaborator), POWELL, SHANE (hasPrincipalInvestigator), POWELL, SHANE (author)
• Format: Dataset
• Language: unknown
• Published: Australian Ocean Data Network
• Subjects: biota; environment; oceans; EARTH SCIENCE > HUMAN DIMENSIONS > ENVIRONMENTAL IMPACTS > CONTAMINANT LEVELS/SPILLS; OIL SPILLS; BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA; BIOSPHERE > ECOLOGICAL DYNAMICS > COMMUNITY DYNAMICS > COMMUNITY STRUCTURE; BACTERIAL DIVERSITY; BACTERIAL COMMUNITY STRUCTURE; DENATURING GRADIENT GEL ELECTROPHORESIS; HYDROCARBONS; NON-METRIC MULTI DIMENSIONAL SCALING PLOTS; SEDIMENT CORERS; FIELD SURVEYS; AMD/AU; CEOS; AMD; CONTINENT > ANTARCTICA; ANTARCTICA > Casey; Brown Bay; O'Brien Bay; Sparkes Bay; Newcomb Bay; GEOGRAPHIC REGION > POLAR; Antarc*; Antarctica
• Online Access: https://researchdata.edu.au/molecular-comparison-bacterial-marine-sediment/2820780

Progress Code: completed Sediment samples which were originally collected as part of ASAC 868 (ASAC_868) are now being investigated using molecular microbial techniques as part of ASAC 1228 (ASAC_1228). Samples were collected in a nested survey design in two hydrocarbon impacted areas and two unimpacted areas. Denaturing gradient gel electrophoresis (DGGE) of a region of the 16S RNA gene was used to investigate the microbial community structure. Banding patterns obtained from the DGGE were transformed into a presence / absence matrix and analysed with a multivariate statistical approach. The download file contains an excel spreadsheet, a csv version of the data, plus a readme file.