BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006
Progress Code: completed This data set contains concentrations of phytoplankton, protozoa, total bacteria and metabolically active bacteria assessed by flow cytometry on transects 12, 1, 3, 5, 7, 9 and 11 of the BROKE-West survey of the Southern Ocean between January and March 2006. Only total bacte...
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ftands:oai:ands.org.au::2819844 2023-10-09T21:44:49+02:00 BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006 Spatial: westlimit=29.99776; southlimit=-69.20696; eastlimit=80.01055; northlimit=-59.79757 Spatial: uplimit=150; downlimit=5 Temporal: From 2006-01-10 to 2006-02-27 https://researchdata.edu.au/broke-west-microbial-australis-20052006/2819844 unknown Australian Ocean Data Network https://researchdata.edu.au/broke-west-microbial-australis-20052006/2819844 BROKE-West_flow_cytometry Dataset DOI AU/AADC > Australian Antarctic Data Centre, Australia biota oceans EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS BACTERIA/ARCHAEA BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON MARINE ECOSYSTEMS > PELAGIC BROKE-West Flow cytometry bacteria FACScan Flow Cytometer Latitude Longitude Transect Number CTD Depth Total bacteria Active bacteria Dead bacteria Protozoa SHIPS R/V AA > R/V Aurora Australis AMD/AU CEOS AMD ACE/CRC OCEAN > SOUTHERN OCEAN CONTINENT > ANTARCTICA GEOGRAPHIC REGION > POLAR dataset ftands 2023-09-18T22:46:14Z Progress Code: completed This data set contains concentrations of phytoplankton, protozoa, total bacteria and metabolically active bacteria assessed by flow cytometry on transects 12, 1, 3, 5, 7, 9 and 11 of the BROKE-West survey of the Southern Ocean between January and March 2006. Only total bacterial concentrations were assessed for transect 11. Between 4 and 12 depths were sampled for marine microbes and concentrations were assessed using FACScan flowcytometer. Phytoplankton were identified and counted based on the autofluorescense of chlorophyll a when excited by the 488 nm laser of the FACScan. Protozoa were identified and counted after staining with the acid vacuole stain Lysotracker Green. Total bacteria were identified and counted using the cell permeant SYTO 13 nucleic stain. Metabolically active bacteria were identified and counted after staining for intracellular esterases with the esterase stain 6CFDA. The fields in this dataset are: Latitude Longitude Transect Number CTD number, flow file Depth (m) Total bacteria (per millilitre) Active bacteria (per millilitre) Dead bacteria (per millilitre) Protozoa (per millilitre) Phytoplankton (per millilitre) This work was completed as part of ASAC project 40 (ASAC_40). Dataset Antarc* Antarctica aurora australis Southern Ocean Research Data Australia (Australian National Data Service - ANDS) Southern Ocean |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
biota oceans EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS BACTERIA/ARCHAEA BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON MARINE ECOSYSTEMS > PELAGIC BROKE-West Flow cytometry bacteria FACScan Flow Cytometer Latitude Longitude Transect Number CTD Depth Total bacteria Active bacteria Dead bacteria Protozoa SHIPS R/V AA > R/V Aurora Australis AMD/AU CEOS AMD ACE/CRC OCEAN > SOUTHERN OCEAN CONTINENT > ANTARCTICA GEOGRAPHIC REGION > POLAR |
spellingShingle |
biota oceans EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS BACTERIA/ARCHAEA BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON MARINE ECOSYSTEMS > PELAGIC BROKE-West Flow cytometry bacteria FACScan Flow Cytometer Latitude Longitude Transect Number CTD Depth Total bacteria Active bacteria Dead bacteria Protozoa SHIPS R/V AA > R/V Aurora Australis AMD/AU CEOS AMD ACE/CRC OCEAN > SOUTHERN OCEAN CONTINENT > ANTARCTICA GEOGRAPHIC REGION > POLAR BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006 |
topic_facet |
biota oceans EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS BACTERIA/ARCHAEA BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON MARINE ECOSYSTEMS > PELAGIC BROKE-West Flow cytometry bacteria FACScan Flow Cytometer Latitude Longitude Transect Number CTD Depth Total bacteria Active bacteria Dead bacteria Protozoa SHIPS R/V AA > R/V Aurora Australis AMD/AU CEOS AMD ACE/CRC OCEAN > SOUTHERN OCEAN CONTINENT > ANTARCTICA GEOGRAPHIC REGION > POLAR |
description |
Progress Code: completed This data set contains concentrations of phytoplankton, protozoa, total bacteria and metabolically active bacteria assessed by flow cytometry on transects 12, 1, 3, 5, 7, 9 and 11 of the BROKE-West survey of the Southern Ocean between January and March 2006. Only total bacterial concentrations were assessed for transect 11. Between 4 and 12 depths were sampled for marine microbes and concentrations were assessed using FACScan flowcytometer. Phytoplankton were identified and counted based on the autofluorescense of chlorophyll a when excited by the 488 nm laser of the FACScan. Protozoa were identified and counted after staining with the acid vacuole stain Lysotracker Green. Total bacteria were identified and counted using the cell permeant SYTO 13 nucleic stain. Metabolically active bacteria were identified and counted after staining for intracellular esterases with the esterase stain 6CFDA. The fields in this dataset are: Latitude Longitude Transect Number CTD number, flow file Depth (m) Total bacteria (per millilitre) Active bacteria (per millilitre) Dead bacteria (per millilitre) Protozoa (per millilitre) Phytoplankton (per millilitre) This work was completed as part of ASAC project 40 (ASAC_40). |
format |
Dataset |
title |
BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006 |
title_short |
BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006 |
title_full |
BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006 |
title_fullStr |
BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006 |
title_full_unstemmed |
BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006 |
title_sort |
broke-west microbial concentrations - voyage 3 of the aurora australis 2005/2006 |
publisher |
Australian Ocean Data Network |
url |
https://researchdata.edu.au/broke-west-microbial-australis-20052006/2819844 |
op_coverage |
Spatial: westlimit=29.99776; southlimit=-69.20696; eastlimit=80.01055; northlimit=-59.79757 Spatial: uplimit=150; downlimit=5 Temporal: From 2006-01-10 to 2006-02-27 |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Antarc* Antarctica aurora australis Southern Ocean |
genre_facet |
Antarc* Antarctica aurora australis Southern Ocean |
op_source |
AU/AADC > Australian Antarctic Data Centre, Australia |
op_relation |
https://researchdata.edu.au/broke-west-microbial-australis-20052006/2819844 BROKE-West_flow_cytometry Dataset DOI |
_version_ |
1779313809618370560 |