| Summary: | Progress Code: completed Statement: Original datasheet was reformatted to fit IPT Biodiversity.AQ standard. The new datasheet "Urchin_climate_change.csv" provides the dataset ID, verbatim latitude and verbatim longitude that were converted to decimal latitude and longitude, also provided. Taxonomical organisation to the lowest taxonomical rank that could be determined, after matched in WoRMS (World Register of Marine Species). Also provides other parameters such as localisation, sampling depth, occurrence, and date of event. The effect of pH, temperature and sperm concentration on the fertilisation of Sterechinus neumayeri was investigated. Adult Sterechinus neumayeri were collected from Ellis Fjord Narrows between December and January 2011-12 and held in the Ecotox Field Aquarium Module until used. Between 3-4 male and female individuals were spawned using 0.5M KCl and gametes were collected separately before being fertilised in treatment. The data set shows the percentage of fertilised and non-fertilised eggs of Sterechinus neumayeri scored at 20h post-fertilisation. Eggs were fertilised in various combinations of pH, temperature and sperm concentration treatments (pH: 8.0 (Control), 7.8 and 7.6; Temperature: 1 degrees C (Control), 3 degrees C and 5 degrees C; Sperm concentration (sperm:egg ratio): 1000:1 (Control), 750:1, 250: 1, 50:1 and 5:1). At 20h post fertilisation, 5 ml aliquot was removed from fertilisation vials and eggs were counted and determined if they were fertilised or not. Seawater parameters of treatments were measured at the start and end of the experiment. Detailed information of the spreadsheets are as follows: Seawater Parameters column headings: Temperature - measured in degrees C , shows the temperature treatments used pH - shows the pH levels used Subheading pH - pH level measured for the day using NIST certified buffers Subheading MV - pH level measured for the day in millivolts Subheading Total pH - total pH level in seawater obtained from MV measurements Subheading Temp - ...
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