| Summary: | Progress Code: completed Statement: Some contamination of hydrocarbon samples occurred during sampling, which test organisms were not exposed to. Contamination levels were therefore excluded from calculations of exposure concentrations. The hydrocarbon content of 0.1% dilutions was unable to be reliably analysed due to accuracy of the equipment and the interfering contamination. These values were extrapolated from analysis of 1% dilutions. It was determined that the extraction method was insufficient for the accurate quantitative recovery of hydrocarbons for the dispersant due to the presence of strongly polar molecules. As such, nominal concentrations were used for all dispersant only treatments and THC data represent the hydrocarbons from the fuel only for chemically dispersed treatments. Purpose This data set aims to assess the toxicity of the two fuel dispersants and the physically and chemically dispersed forms of the three fuels to the common Antarctic amphipod Paramoera walkeri, with additional data to assess differences in toxicity between adult and juvenile life stages. The data set is intended to inform management decisions for marine fuel spills in Antarctica. This metadata record contains the results from bioassays conducted to show the response of the common Antarctic amphipod, Paramoera walkeri to contamination from combinations of Special Antarctic Blend (SAB) diesel, Marine Gas Oil (MGO) and Intermediate Fuel Oil (IFO 180), chemically dispersed with fuel dispersants Ardrox 6120 and Slickgone NS. Fuel only water accommodated fractions (WAF), chemically enhanced water accommodated fractions (CEWAF) and dispersant only treatments were prepared following the methods in Singer et al. (2000) with adaptations from Barron and Ka’aihue (2003). WAF was made using the ratio of 1: 25 (v/v), fuel to filtered seawater (FSW) following the methods of Brown et al. (in prep). Ratios for chemically dispersed treatments were 1: 100 (v/v), fuel to FSW and 1: 20 (v/v) dispersant to fuel. Dispersant only ...
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