| Summary: | Progress Code: completed Statement: This is based on unreplicated data, standard errors are calculated based on microscope field of view pseudoreplicates. Data grouped per MiSeq Sequencer run not per sample, a list of samples in each run are listed against the accession. Purpose A minicosm experiment was conducted on a natural microbial community to investigate the effect of ocean acidification on nearshore Antarctic marine microbes, and to determine if there is a CO2 level at which the structure and function of the community significantly changes. In Hancock et al., 2018, we found that increased CO2 levels greater than 1000μatm significant changed the microbial community favouring smaller phytoplankton over large diatoms and Phaeocystis antarctica. This supports the findings of previous minicosm experiments conducted at the same site (Davidson et al., 2016; Thomson et al., 2016) and shows there is a consistent response and CO2 threshold level (1000μatm) in Prydz Bay, East Antarctica that is consistent both across a season but also between years despite different starting community composition and availability of nutrients (Hancock et al., 2018). However, to date there has been no investigation on the effect of CO2 on the composition of prokaryotes and picoeukaryotes in the Prydz Bay minicosms experiments. This chapter builds on the findings of Hancock et al., 2018 using phylogenetic gene marker sequencing to investigate the effect of ocean acidification on the microbial community during the minicosm experiment. Data from 18S rDNA gene sequences is used to analyse the effect of increased CO2 on large eukaryotes (greater than 3.0μm) to see if there is agreement between compositional data collected via traditional microscopy techniques and molecular methods. Furthermore, the 18S and 16S rDNA data is used to investigate the previously unknown effect of increased CO2 on the picoplankton and prokaryotic community composition during the experiment. Experimental Design A six-level, dose-response ocean ...
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