Prokaryote 16S rRNA sequence data from antFOCE biofilms
Progress Code: completed This metadata record contains an Excel spreadsheet with Operational Taxonomic Units (OTUs) gained from 16S rRNA gene sequencing of prokaryotes sampled from Biofilm slides deployed as part of the antFOCE experiment in the austral summer of 2014/15 at Casey station, East Antar...
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Australian Ocean Data Network
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Online Access: | https://researchdata.edu.au/prokaryote-16s-rrna-antfoce-biofilms/2818410 |
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ftands:oai:ands.org.au::2818410 2023-10-09T21:45:11+02:00 Prokaryote 16S rRNA sequence data from antFOCE biofilms Spatial: westlimit=109.74121; southlimit=-66.53416; eastlimit=111.21582; northlimit=-65.96417 Temporal: From 2014-12-28 to 2015-03-04 https://researchdata.edu.au/prokaryote-16s-rrna-antfoce-biofilms/2818410 unknown Australian Ocean Data Network https://researchdata.edu.au/prokaryote-16s-rrna-antfoce-biofilms/2818410 AAS_4127_antFOCE_Biofilms_Prokaryotes Dataset DOI AU/AADC > Australian Antarctic Data Centre, Australia biota oceans EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > BENTHIC BIOFILM PROKARYOTE OCEAN ACIDIFICATION ADS > Automated DNA Sequencer PH METERS FIELD INVESTIGATION LABORATORY AMD/AU AMD CEOS GEOGRAPHIC REGION > POLAR OCEAN > SOUTHERN OCEAN CONTINENT > ANTARCTICA dataset ftands 2023-09-18T22:44:27Z Progress Code: completed This metadata record contains an Excel spreadsheet with Operational Taxonomic Units (OTUs) gained from 16S rRNA gene sequencing of prokaryotes sampled from Biofilm slides deployed as part of the antFOCE experiment in the austral summer of 2014/15 at Casey station, East Antarctica. Refer to antFOCE report section 4.5.3 for deployment, sampling and analysis details. https://data.aad.gov.au/metadata/records/AAS_4127_antFOCE_Project4127 Sampling design 2 trays of 8 horizontal standard glass microscope slides (72 x 25 mm) per chamber. Four of the glass slides were scored with a diamond pencil approximately 18 mm from the right hand end of the slide and deployed scored side up. The remaining four slides were unmodified. Slides were sampled at: * Tmid - one tray per chamber / open plot. The sampled try was repopulated with fresh slides and redeployed * Tend – 2 slides trays per chamber / open plot. Sampling procedure After 31 days deployment, 1 slide tray per chamber / open plot was sampled. At Tend both trays in each chamber / open plot were sampled. To minimize disturbance while being raised to the surface, each tray was removed from the tray holder by divers and placed in a seawater filled container with a lid. On the surface, slides were removed from the tray using ethanol sterilized forceps. The four unscoured slides per chamber / open plot were placed in a plastic microscope slide holder with a sealable lid. The scoured slides were placed individually in 70 ml plastic sample jars. Lab procedure - Casey The slide holder (4 unscoured slides) from each chamber / open plot was frozen at -20C immediately upon return to the lab. The scoured slides were preserved in sea water containing 1% final concentration glutaraldehyde in separate jars. Preservation Issue: Scoured slides were not refrigerated, either at Casey, during RTA or in Kingston before the 26th Nov 2015, when they were transferred to the 4C Cold Store. antFOCE Background The antFOCE experimental system was deployed in O'Brien Bay, ... Dataset Antarc* Antarctica East Antarctica Ocean acidification Southern Ocean Research Data Australia (Australian National Data Service - ANDS) Southern Ocean East Antarctica Austral Casey Station ENVELOPE(110.528,110.528,-66.282,-66.282) O'Brien Bay ENVELOPE(110.524,110.524,-66.302,-66.302) |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
biota oceans EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > BENTHIC BIOFILM PROKARYOTE OCEAN ACIDIFICATION ADS > Automated DNA Sequencer PH METERS FIELD INVESTIGATION LABORATORY AMD/AU AMD CEOS GEOGRAPHIC REGION > POLAR OCEAN > SOUTHERN OCEAN CONTINENT > ANTARCTICA |
spellingShingle |
biota oceans EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > BENTHIC BIOFILM PROKARYOTE OCEAN ACIDIFICATION ADS > Automated DNA Sequencer PH METERS FIELD INVESTIGATION LABORATORY AMD/AU AMD CEOS GEOGRAPHIC REGION > POLAR OCEAN > SOUTHERN OCEAN CONTINENT > ANTARCTICA Prokaryote 16S rRNA sequence data from antFOCE biofilms |
topic_facet |
biota oceans EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > BENTHIC BIOFILM PROKARYOTE OCEAN ACIDIFICATION ADS > Automated DNA Sequencer PH METERS FIELD INVESTIGATION LABORATORY AMD/AU AMD CEOS GEOGRAPHIC REGION > POLAR OCEAN > SOUTHERN OCEAN CONTINENT > ANTARCTICA |
description |
Progress Code: completed This metadata record contains an Excel spreadsheet with Operational Taxonomic Units (OTUs) gained from 16S rRNA gene sequencing of prokaryotes sampled from Biofilm slides deployed as part of the antFOCE experiment in the austral summer of 2014/15 at Casey station, East Antarctica. Refer to antFOCE report section 4.5.3 for deployment, sampling and analysis details. https://data.aad.gov.au/metadata/records/AAS_4127_antFOCE_Project4127 Sampling design 2 trays of 8 horizontal standard glass microscope slides (72 x 25 mm) per chamber. Four of the glass slides were scored with a diamond pencil approximately 18 mm from the right hand end of the slide and deployed scored side up. The remaining four slides were unmodified. Slides were sampled at: * Tmid - one tray per chamber / open plot. The sampled try was repopulated with fresh slides and redeployed * Tend – 2 slides trays per chamber / open plot. Sampling procedure After 31 days deployment, 1 slide tray per chamber / open plot was sampled. At Tend both trays in each chamber / open plot were sampled. To minimize disturbance while being raised to the surface, each tray was removed from the tray holder by divers and placed in a seawater filled container with a lid. On the surface, slides were removed from the tray using ethanol sterilized forceps. The four unscoured slides per chamber / open plot were placed in a plastic microscope slide holder with a sealable lid. The scoured slides were placed individually in 70 ml plastic sample jars. Lab procedure - Casey The slide holder (4 unscoured slides) from each chamber / open plot was frozen at -20C immediately upon return to the lab. The scoured slides were preserved in sea water containing 1% final concentration glutaraldehyde in separate jars. Preservation Issue: Scoured slides were not refrigerated, either at Casey, during RTA or in Kingston before the 26th Nov 2015, when they were transferred to the 4C Cold Store. antFOCE Background The antFOCE experimental system was deployed in O'Brien Bay, ... |
format |
Dataset |
title |
Prokaryote 16S rRNA sequence data from antFOCE biofilms |
title_short |
Prokaryote 16S rRNA sequence data from antFOCE biofilms |
title_full |
Prokaryote 16S rRNA sequence data from antFOCE biofilms |
title_fullStr |
Prokaryote 16S rRNA sequence data from antFOCE biofilms |
title_full_unstemmed |
Prokaryote 16S rRNA sequence data from antFOCE biofilms |
title_sort |
prokaryote 16s rrna sequence data from antfoce biofilms |
publisher |
Australian Ocean Data Network |
url |
https://researchdata.edu.au/prokaryote-16s-rrna-antfoce-biofilms/2818410 |
op_coverage |
Spatial: westlimit=109.74121; southlimit=-66.53416; eastlimit=111.21582; northlimit=-65.96417 Temporal: From 2014-12-28 to 2015-03-04 |
long_lat |
ENVELOPE(110.528,110.528,-66.282,-66.282) ENVELOPE(110.524,110.524,-66.302,-66.302) |
geographic |
Southern Ocean East Antarctica Austral Casey Station O'Brien Bay |
geographic_facet |
Southern Ocean East Antarctica Austral Casey Station O'Brien Bay |
genre |
Antarc* Antarctica East Antarctica Ocean acidification Southern Ocean |
genre_facet |
Antarc* Antarctica East Antarctica Ocean acidification Southern Ocean |
op_source |
AU/AADC > Australian Antarctic Data Centre, Australia |
op_relation |
https://researchdata.edu.au/prokaryote-16s-rrna-antfoce-biofilms/2818410 AAS_4127_antFOCE_Biofilms_Prokaryotes Dataset DOI |
_version_ |
1779316458477584384 |