Eukaryotic 18S rDNA PCR amplification and high-throughput sequencing of antFOCE Biofilms
Progress Code: completed Statement: An updated dataset was provided by Laurence Clarke on 2019-09-27 (two files were added for DNA sequences and taxonomy based on those sequences). The previous dataset was referenced by a different DOI - doi:10.4225/15/5aaf4307ccb36 This metadata record contains an...
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Australian Ocean Data Network
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Online Access: | https://researchdata.edu.au/eukaryotic-18s-rdna-antfoce-biofilms/2818404 |
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ftands:oai:ands.org.au::2818404 2023-10-09T21:45:05+02:00 Eukaryotic 18S rDNA PCR amplification and high-throughput sequencing of antFOCE Biofilms Spatial: westlimit=109.62036; southlimit=-66.68817; eastlimit=111.38062; northlimit=-65.89858 Temporal: From 2014-12-28 to 2015-03-04 https://researchdata.edu.au/eukaryotic-18s-rdna-antfoce-biofilms/2818404 unknown Australian Ocean Data Network https://researchdata.edu.au/eukaryotic-18s-rdna-antfoce-biofilms/2818404 AAS_4127_antFOCE_Biofilms_Eukaryotes Dataset DOI AU/AADC > Australian Antarctic Data Centre, Australia biota oceans EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > BENTHIC OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE EUKARYOTES BIOFILMS OCEAN ACIDIFICATION ADS > Automated DNA Sequencer MICROSCOPES FIELD SURVEYS LABORATORY AMD/AU AMD CEOS GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA OCEAN > SOUTHERN OCEAN dataset ftands 2023-09-18T22:44:27Z Progress Code: completed Statement: An updated dataset was provided by Laurence Clarke on 2019-09-27 (two files were added for DNA sequences and taxonomy based on those sequences). The previous dataset was referenced by a different DOI - doi:10.4225/15/5aaf4307ccb36 This metadata record contains an Excel spreadsheet with Operational Taxonomic Units (OTUs) gained from Eukaryotic 18S rDNA PCR amplification and high-throughput sequencing of samples from Biofilm slides deployed as part of the antFOCE experiment in the austral summer of 2014/15 at Casey station, East Antarctica. Refer to antFOCE report section 4.5.3 for deployment, sampling and analysis details. https://data.aad.gov.au/metadata/records/AAS_4127_antFOCE_Project4127 Sampling design 2 trays of 8 horizontal standard glass microscope slides (72 x 25 mm) per chamber. Four of the glass slides were scored with a diamond pencil approximately 18 mm from the right hand end of the slide and deployed scored side up. The remaining four slides were unmodified. Slides were sampled at: - Tmid - one tray per chamber / open plot. The sampled try was repopulated with fresh slides and redeployed - Tend – 2 slides trays per chamber / open plot. Sampling procedure After 31 days deployment, 1 slide tray per chamber / open plot was sampled. At Tend both trays in each chamber / open plot were sampled. To minimize disturbance while being raised to the surface, each tray was removed from the tray holder by divers and placed in a seawater filled container with a lid. On the surface, slides were removed from the tray using ethanol sterilized forceps. The four unscoured slides per chamber / open plot were placed in a plastic microscope slide holder with a sealable lid. The scoured slides were placed individually in 70 ml plastic sample jars. Lab procedure - Casey The slide holder (4 unscoured slides) from each chamber / open plot was frozen at -20C immediately upon return to the lab. The scoured slides were preserved in sea water containing 1% final concentration ... Dataset Antarc* Antarctica East Antarctica Ocean acidification Southern Ocean Research Data Australia (Australian National Data Service - ANDS) Southern Ocean East Antarctica Austral Casey Station ENVELOPE(110.528,110.528,-66.282,-66.282) |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
biota oceans EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > BENTHIC OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE EUKARYOTES BIOFILMS OCEAN ACIDIFICATION ADS > Automated DNA Sequencer MICROSCOPES FIELD SURVEYS LABORATORY AMD/AU AMD CEOS GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA OCEAN > SOUTHERN OCEAN |
spellingShingle |
biota oceans EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > BENTHIC OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE EUKARYOTES BIOFILMS OCEAN ACIDIFICATION ADS > Automated DNA Sequencer MICROSCOPES FIELD SURVEYS LABORATORY AMD/AU AMD CEOS GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA OCEAN > SOUTHERN OCEAN Eukaryotic 18S rDNA PCR amplification and high-throughput sequencing of antFOCE Biofilms |
topic_facet |
biota oceans EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > BENTHIC OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE EUKARYOTES BIOFILMS OCEAN ACIDIFICATION ADS > Automated DNA Sequencer MICROSCOPES FIELD SURVEYS LABORATORY AMD/AU AMD CEOS GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA OCEAN > SOUTHERN OCEAN |
description |
Progress Code: completed Statement: An updated dataset was provided by Laurence Clarke on 2019-09-27 (two files were added for DNA sequences and taxonomy based on those sequences). The previous dataset was referenced by a different DOI - doi:10.4225/15/5aaf4307ccb36 This metadata record contains an Excel spreadsheet with Operational Taxonomic Units (OTUs) gained from Eukaryotic 18S rDNA PCR amplification and high-throughput sequencing of samples from Biofilm slides deployed as part of the antFOCE experiment in the austral summer of 2014/15 at Casey station, East Antarctica. Refer to antFOCE report section 4.5.3 for deployment, sampling and analysis details. https://data.aad.gov.au/metadata/records/AAS_4127_antFOCE_Project4127 Sampling design 2 trays of 8 horizontal standard glass microscope slides (72 x 25 mm) per chamber. Four of the glass slides were scored with a diamond pencil approximately 18 mm from the right hand end of the slide and deployed scored side up. The remaining four slides were unmodified. Slides were sampled at: - Tmid - one tray per chamber / open plot. The sampled try was repopulated with fresh slides and redeployed - Tend – 2 slides trays per chamber / open plot. Sampling procedure After 31 days deployment, 1 slide tray per chamber / open plot was sampled. At Tend both trays in each chamber / open plot were sampled. To minimize disturbance while being raised to the surface, each tray was removed from the tray holder by divers and placed in a seawater filled container with a lid. On the surface, slides were removed from the tray using ethanol sterilized forceps. The four unscoured slides per chamber / open plot were placed in a plastic microscope slide holder with a sealable lid. The scoured slides were placed individually in 70 ml plastic sample jars. Lab procedure - Casey The slide holder (4 unscoured slides) from each chamber / open plot was frozen at -20C immediately upon return to the lab. The scoured slides were preserved in sea water containing 1% final concentration ... |
format |
Dataset |
title |
Eukaryotic 18S rDNA PCR amplification and high-throughput sequencing of antFOCE Biofilms |
title_short |
Eukaryotic 18S rDNA PCR amplification and high-throughput sequencing of antFOCE Biofilms |
title_full |
Eukaryotic 18S rDNA PCR amplification and high-throughput sequencing of antFOCE Biofilms |
title_fullStr |
Eukaryotic 18S rDNA PCR amplification and high-throughput sequencing of antFOCE Biofilms |
title_full_unstemmed |
Eukaryotic 18S rDNA PCR amplification and high-throughput sequencing of antFOCE Biofilms |
title_sort |
eukaryotic 18s rdna pcr amplification and high-throughput sequencing of antfoce biofilms |
publisher |
Australian Ocean Data Network |
url |
https://researchdata.edu.au/eukaryotic-18s-rdna-antfoce-biofilms/2818404 |
op_coverage |
Spatial: westlimit=109.62036; southlimit=-66.68817; eastlimit=111.38062; northlimit=-65.89858 Temporal: From 2014-12-28 to 2015-03-04 |
long_lat |
ENVELOPE(110.528,110.528,-66.282,-66.282) |
geographic |
Southern Ocean East Antarctica Austral Casey Station |
geographic_facet |
Southern Ocean East Antarctica Austral Casey Station |
genre |
Antarc* Antarctica East Antarctica Ocean acidification Southern Ocean |
genre_facet |
Antarc* Antarctica East Antarctica Ocean acidification Southern Ocean |
op_source |
AU/AADC > Australian Antarctic Data Centre, Australia |
op_relation |
https://researchdata.edu.au/eukaryotic-18s-rdna-antfoce-biofilms/2818404 AAS_4127_antFOCE_Biofilms_Eukaryotes Dataset DOI |
_version_ |
1779315848744271872 |