Eukaryotic 18S rDNA PCR amplification and high-throughput sequencing of antFOCE Biofilms

• Format: Dataset
• Language: unknown
• Published: Australian Ocean Data Network
• Subjects: biota; oceans; EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > BENTHIC; OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE; EUKARYOTES; BIOFILMS; OCEAN ACIDIFICATION; ADS > Automated DNA Sequencer; MICROSCOPES; FIELD SURVEYS; LABORATORY; AMD/AU; AMD; CEOS; GEOGRAPHIC REGION > POLAR; CONTINENT > ANTARCTICA; OCEAN > SOUTHERN OCEAN; Southern Ocean; East Antarctica; Austral; Casey Station; Antarc*; Antarctica; Ocean acidification
• Online Access: https://researchdata.edu.au/eukaryotic-18s-rdna-antfoce-biofilms/2818404

Progress Code: completed Statement: An updated dataset was provided by Laurence Clarke on 2019-09-27 (two files were added for DNA sequences and taxonomy based on those sequences). The previous dataset was referenced by a different DOI - doi:10.4225/15/5aaf4307ccb36 This metadata record contains an Excel spreadsheet with Operational Taxonomic Units (OTUs) gained from Eukaryotic 18S rDNA PCR amplification and high-throughput sequencing of samples from Biofilm slides deployed as part of the antFOCE experiment in the austral summer of 2014/15 at Casey station, East Antarctica. Refer to antFOCE report section 4.5.3 for deployment, sampling and analysis details. https://data.aad.gov.au/metadata/records/AAS_4127_antFOCE_Project4127 Sampling design 2 trays of 8 horizontal standard glass microscope slides (72 x 25 mm) per chamber. Four of the glass slides were scored with a diamond pencil approximately 18 mm from the right hand end of the slide and deployed scored side up. The remaining four slides were unmodified. Slides were sampled at: - Tmid - one tray per chamber / open plot. The sampled try was repopulated with fresh slides and redeployed - Tend – 2 slides trays per chamber / open plot. Sampling procedure After 31 days deployment, 1 slide tray per chamber / open plot was sampled. At Tend both trays in each chamber / open plot were sampled. To minimize disturbance while being raised to the surface, each tray was removed from the tray holder by divers and placed in a seawater filled container with a lid. On the surface, slides were removed from the tray using ethanol sterilized forceps. The four unscoured slides per chamber / open plot were placed in a plastic microscope slide holder with a sealable lid. The scoured slides were placed individually in 70 ml plastic sample jars. Lab procedure - Casey The slide holder (4 unscoured slides) from each chamber / open plot was frozen at -20C immediately upon return to the lab. The scoured slides were preserved in sea water containing 1% final concentration ...