K-Axis mesopelagic fish DNA-based diet analysis
Progress Code: completed Statement: Many COI samples have too few sequencing reads (counts) to reliably estimate diet based on this data. See detail in Clarke et al. (2018), Deep Sea Research II, https://doi.org/10.1016/j.dsr2.2018.09.001. Purpose Characterise Southern Ocean mesopelagic fish diets u...
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ftands:oai:ands.org.au::2818236 2023-10-09T21:50:02+02:00 K-Axis mesopelagic fish DNA-based diet analysis Spatial: westlimit=80.423784; southlimit=-64.492642; eastlimit=90.210047; northlimit=-58.86768 Spatial: uplimit=1000; downlimit=0 Temporal: From 2016-02-03 to 2016-03-07 https://researchdata.edu.au/k-axis-mesopelagic-diet-analysis/2818236 unknown Australian Ocean Data Network https://researchdata.edu.au/k-axis-mesopelagic-diet-analysis/2818236 AAS_4313_KAXIS_FISH_DIET Dataset DOI AU/AADC > Australian Antarctic Data Centre, Australia biota oceans EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > FISH PROTISTS > AMOEBOIDS > RADIOLARIANS ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS > EUPHAUSIIDS (KRILL) COPEPODS DNA 18S RIBOSOMAL DNA COI (CYTOCHROME OXIDASE I) ILLUMINA MISEQ MIDOC ADS > Automated DNA Sequencer R/V AA > R/V Aurora Australis AMD AMD/AU CEOS GEOGRAPHIC REGION > POLAR OCEAN > SOUTHERN OCEAN > SOUTHERN KERGUELEN AXIS PRINCESS ELIZABETH TROUGH dataset ftands 2023-09-18T22:44:04Z Progress Code: completed Statement: Many COI samples have too few sequencing reads (counts) to reliably estimate diet based on this data. See detail in Clarke et al. (2018), Deep Sea Research II, https://doi.org/10.1016/j.dsr2.2018.09.001. Purpose Characterise Southern Ocean mesopelagic fish diets using DNA data. In addition, test for differences in diet between fish species and regions (north and south of Kerguelen Axis voyage). High-throughput DNA-sequencing data for mesopelagic fish stomach contents sampled during the Kerguelen Axis voyage (January-Februay 2016). Mesopelagic fish form an important link between zooplankton and higher trophic levels in Southern Ocean food webs, however their diets are poorly known. Most of the dietary information available comes from morphological analysis of stomach contents and to a lesser extent fatty acid and stable isotopes. DNA sequencing could substantially improve our knowledge of mesopelagic fish diets, but has not previously been applied. We used high-throughput DNA sequencing (HTS) of the 18S ribosomal DNA and mitochondrial cytochrome oxidase I (COI) to characterise stomach contents of four myctophid and one bathylagid species collected at the southern extension of the Kerguelen Plateau (southern Kerguelen Axis), one of the most productive regions in the Indian sector of the Southern Ocean. Diets of the four myctophid species were dominated by amphipods, euphausiids and copepods, whereas radiolarians and siphonophores contributed a much greater proportion of HTS reads for Bathylagus sp. Analysis of mitochondrial COI showed that all species preyed on Thysanoessa macrura, but Euphausia superba was only detected in the stomach contents of myctophids. Size-based shifts in diet were apparent, with larger individuals of both bathylagid and myctophid species more likely to consume euphausiids, but we found little evidence for regional differences in diet composition for each species over the survey area. The presence of DNA from coelenterates and other gelatinous prey ... Dataset aurora australis Euphausia superba Southern Ocean Thysanoessa macrura Copepods Research Data Australia (Australian National Data Service - ANDS) Southern Ocean Kerguelen Indian Princess Elizabeth Trough ENVELOPE(83.000,83.000,-64.167,-64.167) |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
biota oceans EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > FISH PROTISTS > AMOEBOIDS > RADIOLARIANS ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS > EUPHAUSIIDS (KRILL) COPEPODS DNA 18S RIBOSOMAL DNA COI (CYTOCHROME OXIDASE I) ILLUMINA MISEQ MIDOC ADS > Automated DNA Sequencer R/V AA > R/V Aurora Australis AMD AMD/AU CEOS GEOGRAPHIC REGION > POLAR OCEAN > SOUTHERN OCEAN > SOUTHERN KERGUELEN AXIS PRINCESS ELIZABETH TROUGH |
spellingShingle |
biota oceans EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > FISH PROTISTS > AMOEBOIDS > RADIOLARIANS ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS > EUPHAUSIIDS (KRILL) COPEPODS DNA 18S RIBOSOMAL DNA COI (CYTOCHROME OXIDASE I) ILLUMINA MISEQ MIDOC ADS > Automated DNA Sequencer R/V AA > R/V Aurora Australis AMD AMD/AU CEOS GEOGRAPHIC REGION > POLAR OCEAN > SOUTHERN OCEAN > SOUTHERN KERGUELEN AXIS PRINCESS ELIZABETH TROUGH K-Axis mesopelagic fish DNA-based diet analysis |
topic_facet |
biota oceans EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > FISH PROTISTS > AMOEBOIDS > RADIOLARIANS ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS > EUPHAUSIIDS (KRILL) COPEPODS DNA 18S RIBOSOMAL DNA COI (CYTOCHROME OXIDASE I) ILLUMINA MISEQ MIDOC ADS > Automated DNA Sequencer R/V AA > R/V Aurora Australis AMD AMD/AU CEOS GEOGRAPHIC REGION > POLAR OCEAN > SOUTHERN OCEAN > SOUTHERN KERGUELEN AXIS PRINCESS ELIZABETH TROUGH |
description |
Progress Code: completed Statement: Many COI samples have too few sequencing reads (counts) to reliably estimate diet based on this data. See detail in Clarke et al. (2018), Deep Sea Research II, https://doi.org/10.1016/j.dsr2.2018.09.001. Purpose Characterise Southern Ocean mesopelagic fish diets using DNA data. In addition, test for differences in diet between fish species and regions (north and south of Kerguelen Axis voyage). High-throughput DNA-sequencing data for mesopelagic fish stomach contents sampled during the Kerguelen Axis voyage (January-Februay 2016). Mesopelagic fish form an important link between zooplankton and higher trophic levels in Southern Ocean food webs, however their diets are poorly known. Most of the dietary information available comes from morphological analysis of stomach contents and to a lesser extent fatty acid and stable isotopes. DNA sequencing could substantially improve our knowledge of mesopelagic fish diets, but has not previously been applied. We used high-throughput DNA sequencing (HTS) of the 18S ribosomal DNA and mitochondrial cytochrome oxidase I (COI) to characterise stomach contents of four myctophid and one bathylagid species collected at the southern extension of the Kerguelen Plateau (southern Kerguelen Axis), one of the most productive regions in the Indian sector of the Southern Ocean. Diets of the four myctophid species were dominated by amphipods, euphausiids and copepods, whereas radiolarians and siphonophores contributed a much greater proportion of HTS reads for Bathylagus sp. Analysis of mitochondrial COI showed that all species preyed on Thysanoessa macrura, but Euphausia superba was only detected in the stomach contents of myctophids. Size-based shifts in diet were apparent, with larger individuals of both bathylagid and myctophid species more likely to consume euphausiids, but we found little evidence for regional differences in diet composition for each species over the survey area. The presence of DNA from coelenterates and other gelatinous prey ... |
format |
Dataset |
title |
K-Axis mesopelagic fish DNA-based diet analysis |
title_short |
K-Axis mesopelagic fish DNA-based diet analysis |
title_full |
K-Axis mesopelagic fish DNA-based diet analysis |
title_fullStr |
K-Axis mesopelagic fish DNA-based diet analysis |
title_full_unstemmed |
K-Axis mesopelagic fish DNA-based diet analysis |
title_sort |
k-axis mesopelagic fish dna-based diet analysis |
publisher |
Australian Ocean Data Network |
url |
https://researchdata.edu.au/k-axis-mesopelagic-diet-analysis/2818236 |
op_coverage |
Spatial: westlimit=80.423784; southlimit=-64.492642; eastlimit=90.210047; northlimit=-58.86768 Spatial: uplimit=1000; downlimit=0 Temporal: From 2016-02-03 to 2016-03-07 |
long_lat |
ENVELOPE(83.000,83.000,-64.167,-64.167) |
geographic |
Southern Ocean Kerguelen Indian Princess Elizabeth Trough |
geographic_facet |
Southern Ocean Kerguelen Indian Princess Elizabeth Trough |
genre |
aurora australis Euphausia superba Southern Ocean Thysanoessa macrura Copepods |
genre_facet |
aurora australis Euphausia superba Southern Ocean Thysanoessa macrura Copepods |
op_source |
AU/AADC > Australian Antarctic Data Centre, Australia |
op_relation |
https://researchdata.edu.au/k-axis-mesopelagic-diet-analysis/2818236 AAS_4313_KAXIS_FISH_DIET Dataset DOI |
_version_ |
1779313104670162944 |