K-Axis eukaryote Operational Taxonomic Units (OTU) table and contextual data
Progress Code: completed Purpose Characterise the eukaryotic plankton community in the Kerguelen Axis region, and identify the ecological drivers of community composition. Sampling Samples were collected on board the RSV Aurora Australis between 22 January and 17 February 2016. The cruise surveyed t...
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Online Access: | https://researchdata.edu.au/k-axis-eukaryote-contextual-data/2818221 |
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ftands:oai:ands.org.au::2818221 2024-09-15T17:46:49+00:00 K-Axis eukaryote Operational Taxonomic Units (OTU) table and contextual data AADC (owner) AADC, DATA OFFICER (distributor) AADC, DATA OFFICER (custodian) AU/AADC > Australian Antarctic Data Centre, Australia (hasAssociationWith) Australian Antarctic Data Centre (publisher) Australian Antarctic Division (sponsor) BISSETT, ANDREW (hasPrincipalInvestigator) CLARKE, LAURENCE J. (hasPrincipalInvestigator) CLARKE, LAURENCE J. (author) DEAGLE, BRUCE E. (collaborator) DEAGLE, BRUCE E. (hasPrincipalInvestigator) Deagle, B.E., Clarke, L.J., Bissett, A. and Bestley, S. (originator) Spatial: westlimit=68.76833333; southlimit=-66.6074; eastlimit=93.57; northlimit=-57.5929 Spatial: uplimit=74; downlimit=5 Temporal: From 2016-01-22 to 2016-02-17 https://researchdata.edu.au/k-axis-eukaryote-contextual-data/2818221 unknown Australian Ocean Data Network https://researchdata.edu.au/k-axis-eukaryote-contextual-data/2818221 AAS_4313_KAXIS_EUK_OTUS AU/AADC > Australian Antarctic Data Centre, Australia biota oceans EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > PLANKTON OCEANS > OCEAN CHEMISTRY > CHLOROPHYLL SALINITY/DENSITY > SALINITY ILLUMINA MISEQ EUKARYOTE 18S RIBOSOMAL RNA ADS > Automated DNA Sequencer NISKIN BOTTLES CTD > Conductivity Temperature Depth LABORATORY R/V AA > R/V Aurora Australis ACE/CRC AMD/AU AMD CEOS GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA OCEAN > SOUTHERN OCEAN > BANZARE BANK PRINCESS ELIZABETH TROUGH dataset ftands 2024-08-06T01:58:58Z Progress Code: completed Purpose Characterise the eukaryotic plankton community in the Kerguelen Axis region, and identify the ecological drivers of community composition. Sampling Samples were collected on board the RSV Aurora Australis between 22 January and 17 February 2016. The cruise surveyed the region south of the Kerguelen Plateau including the Princess Elizabeth Trough and BANZARE Bank in a series of eight transects covering 8165 km. Plankton communities were collected at 45 conductivity temperature depth (CTD) stations and seven additional underway stations, with biological replicates collected at two stations (52 independent sites). Surface water was sampled from 4 plus or minus 2 m depth using the uncontaminated seawater line. Deep Chlorophyll Maximum (DCM, 10-74 m) water samples were obtained using 10 L Niskin bottles mounted on a Seabird 911+ CTD. Plankton communities were size-fractionated by sequentially filtering 10 L seawater through 25 mm 20 micron (nylon) and 5 micron filters (PVDF), and 0.45 micron Sterivex filters (PVDF). Filters were stored frozen at -80 °C. DNA extraction and high-throughput sequencing DNA was extracted from half of each filter using the MoBio PowerSoil DNA Isolation kit at the Australian Genome Research Facility (AGRF, Adelaide, Australia; http://www.agrf.org.au). The V4 region of the 18S rDNA (approximately 380 bp excluding primers) was PCR-amplified using universal eukaryotic primers from all extracts and sequenced on an Illumina MiSeq v2 (2 x 250 bp paired-end) following the Ocean Sampling Day protocol (Piredda et al. 2017). Amplicon library preparation and high-throughput sequencing were carried out at the Ramaciotti Centre for Genomics (Sydney, Australia). Sequence analysis, OTU picking and assignment followed the Biomes of Australian Soil Environments (BASE) workflow (Bissett et al. 2016). Taxonomy was assigned to OTUs based on the PR2 database using the ‘classify.seqs’ command in mothur version 1.31.2 with default settings and a bootstrap cut-off of 60%. OTUs ... Dataset Antarc* Antarctica aurora australis Southern Ocean Research Data Australia (Australian National Data Service - ANDS) |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
biota oceans EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > PLANKTON OCEANS > OCEAN CHEMISTRY > CHLOROPHYLL SALINITY/DENSITY > SALINITY ILLUMINA MISEQ EUKARYOTE 18S RIBOSOMAL RNA ADS > Automated DNA Sequencer NISKIN BOTTLES CTD > Conductivity Temperature Depth LABORATORY R/V AA > R/V Aurora Australis ACE/CRC AMD/AU AMD CEOS GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA OCEAN > SOUTHERN OCEAN > BANZARE BANK PRINCESS ELIZABETH TROUGH |
spellingShingle |
biota oceans EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > PLANKTON OCEANS > OCEAN CHEMISTRY > CHLOROPHYLL SALINITY/DENSITY > SALINITY ILLUMINA MISEQ EUKARYOTE 18S RIBOSOMAL RNA ADS > Automated DNA Sequencer NISKIN BOTTLES CTD > Conductivity Temperature Depth LABORATORY R/V AA > R/V Aurora Australis ACE/CRC AMD/AU AMD CEOS GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA OCEAN > SOUTHERN OCEAN > BANZARE BANK PRINCESS ELIZABETH TROUGH K-Axis eukaryote Operational Taxonomic Units (OTU) table and contextual data |
topic_facet |
biota oceans EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > PLANKTON OCEANS > OCEAN CHEMISTRY > CHLOROPHYLL SALINITY/DENSITY > SALINITY ILLUMINA MISEQ EUKARYOTE 18S RIBOSOMAL RNA ADS > Automated DNA Sequencer NISKIN BOTTLES CTD > Conductivity Temperature Depth LABORATORY R/V AA > R/V Aurora Australis ACE/CRC AMD/AU AMD CEOS GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA OCEAN > SOUTHERN OCEAN > BANZARE BANK PRINCESS ELIZABETH TROUGH |
description |
Progress Code: completed Purpose Characterise the eukaryotic plankton community in the Kerguelen Axis region, and identify the ecological drivers of community composition. Sampling Samples were collected on board the RSV Aurora Australis between 22 January and 17 February 2016. The cruise surveyed the region south of the Kerguelen Plateau including the Princess Elizabeth Trough and BANZARE Bank in a series of eight transects covering 8165 km. Plankton communities were collected at 45 conductivity temperature depth (CTD) stations and seven additional underway stations, with biological replicates collected at two stations (52 independent sites). Surface water was sampled from 4 plus or minus 2 m depth using the uncontaminated seawater line. Deep Chlorophyll Maximum (DCM, 10-74 m) water samples were obtained using 10 L Niskin bottles mounted on a Seabird 911+ CTD. Plankton communities were size-fractionated by sequentially filtering 10 L seawater through 25 mm 20 micron (nylon) and 5 micron filters (PVDF), and 0.45 micron Sterivex filters (PVDF). Filters were stored frozen at -80 °C. DNA extraction and high-throughput sequencing DNA was extracted from half of each filter using the MoBio PowerSoil DNA Isolation kit at the Australian Genome Research Facility (AGRF, Adelaide, Australia; http://www.agrf.org.au). The V4 region of the 18S rDNA (approximately 380 bp excluding primers) was PCR-amplified using universal eukaryotic primers from all extracts and sequenced on an Illumina MiSeq v2 (2 x 250 bp paired-end) following the Ocean Sampling Day protocol (Piredda et al. 2017). Amplicon library preparation and high-throughput sequencing were carried out at the Ramaciotti Centre for Genomics (Sydney, Australia). Sequence analysis, OTU picking and assignment followed the Biomes of Australian Soil Environments (BASE) workflow (Bissett et al. 2016). Taxonomy was assigned to OTUs based on the PR2 database using the ‘classify.seqs’ command in mothur version 1.31.2 with default settings and a bootstrap cut-off of 60%. OTUs ... |
author2 |
AADC (owner) AADC, DATA OFFICER (distributor) AADC, DATA OFFICER (custodian) AU/AADC > Australian Antarctic Data Centre, Australia (hasAssociationWith) Australian Antarctic Data Centre (publisher) Australian Antarctic Division (sponsor) BISSETT, ANDREW (hasPrincipalInvestigator) CLARKE, LAURENCE J. (hasPrincipalInvestigator) CLARKE, LAURENCE J. (author) DEAGLE, BRUCE E. (collaborator) DEAGLE, BRUCE E. (hasPrincipalInvestigator) Deagle, B.E., Clarke, L.J., Bissett, A. and Bestley, S. (originator) |
format |
Dataset |
title |
K-Axis eukaryote Operational Taxonomic Units (OTU) table and contextual data |
title_short |
K-Axis eukaryote Operational Taxonomic Units (OTU) table and contextual data |
title_full |
K-Axis eukaryote Operational Taxonomic Units (OTU) table and contextual data |
title_fullStr |
K-Axis eukaryote Operational Taxonomic Units (OTU) table and contextual data |
title_full_unstemmed |
K-Axis eukaryote Operational Taxonomic Units (OTU) table and contextual data |
title_sort |
k-axis eukaryote operational taxonomic units (otu) table and contextual data |
publisher |
Australian Ocean Data Network |
url |
https://researchdata.edu.au/k-axis-eukaryote-contextual-data/2818221 |
op_coverage |
Spatial: westlimit=68.76833333; southlimit=-66.6074; eastlimit=93.57; northlimit=-57.5929 Spatial: uplimit=74; downlimit=5 Temporal: From 2016-01-22 to 2016-02-17 |
genre |
Antarc* Antarctica aurora australis Southern Ocean |
genre_facet |
Antarc* Antarctica aurora australis Southern Ocean |
op_source |
AU/AADC > Australian Antarctic Data Centre, Australia |
op_relation |
https://researchdata.edu.au/k-axis-eukaryote-contextual-data/2818221 AAS_4313_KAXIS_EUK_OTUS |
_version_ |
1810495206846365696 |