Sediment pigment analysis at Casey Station, 2014-2015
Progress Code: completed Statement: These data are considered to be of high quality. Purpose This is an antFOCE dataset, collected in order to determine community composition from a pigment perspective. General description: The associated file contains sediment pigment data from the antFOCE project...
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ftands:oai:ands.org.au::2818176 2023-10-09T21:46:50+02:00 Sediment pigment analysis at Casey Station, 2014-2015 Spatial: westlimit=96.32813; southlimit=-69.41124; eastlimit=120.9375; northlimit=-62.26792 Spatial: uplimit=15; downlimit=0 Temporal: From 2014-12-01 to 2015-03-01 https://researchdata.edu.au/sediment-pigment-analysis-2014-2015/2818176 unknown Australian Ocean Data Network https://researchdata.edu.au/sediment-pigment-analysis-2014-2015/2818176 AAS_4127_Sediment_pigment_data Dataset DOI AU/AADC > Australian Antarctic Data Centre, Australia oceans biota EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PIGMENTS CARBON DIOXIDE ANTFOCE OCEAN ACIDIFICATION LABORATORY AMD CEOS GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA > CASEY OCEAN > SOUTHERN OCEAN dataset ftands 2023-09-18T22:44:04Z Progress Code: completed Statement: These data are considered to be of high quality. Purpose This is an antFOCE dataset, collected in order to determine community composition from a pigment perspective. General description: The associated file contains sediment pigment data from the antFOCE project 4127. Units: all pigment data in ug/g, 0 = below detection limit of HPLC. Sample collection details: At the start and end of the antFOCE experiment, four sediment core samples were taken from inside and outside each chamber or open plot by divers. The top 1 cm of the cores was then removed and placed in the dark, first at -20ºC for 2 hours, then at -80ºC until analysis at the Australian Antarctic division. Pigment analysis Frozen samples were transported under liquid N2 to a freeze drier (Dynavac, model FD-5), in pre-chilled flasks with a small amount of liquid N2 added. Custom made plumbing fitted to the freeze drier enabled samples to be purged with N2 to prevent photo-oxidation up until solvent extraction. Prior to pigment extraction five 2 g stainless steel ball bearings were added to homogenise the freeze dried sediment. The samples were bead beaten for 1 minute (Biospec products). Subsamples (~0.05 g) were immediately transferred to cryotubes with 700 µl of dimethylformamide (DMF) for two hours. Samples were kept at -80ºC and under a safe light (IFORD 902) at all times. All pigment concentrations are standardised to sediment weight. Pigments were extracted with dimethylformamide (DMF 700 µl) over a two hour period at -20ºC. Zirconia beads, and 100 µl of Apo 8 and an internal standard were added to each sub-sample. After a two hour extraction, sub-samples were bead beaten for 20 seconds and then placed in a centrifuge with filter cartridge inserts for 14 minutes at 2500 rpm at -9ºC to separate the solvent from the sediment. The supernatant was transferred into to a vial and placed in a precooled rpHPLC autosampler. The rpHPLC system used is described in Hodgson et al. (1997). Pigment detection was at 435, ... Dataset Antarc* Antarctic Antarctica Australian Antarctic Division Ocean acidification Southern Ocean Research Data Australia (Australian National Data Service - ANDS) Antarctic Southern Ocean Casey Station ENVELOPE(110.528,110.528,-66.282,-66.282) Hodgson ENVELOPE(166.083,166.083,-78.117,-78.117) |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
oceans biota EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PIGMENTS CARBON DIOXIDE ANTFOCE OCEAN ACIDIFICATION LABORATORY AMD CEOS GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA > CASEY OCEAN > SOUTHERN OCEAN |
spellingShingle |
oceans biota EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PIGMENTS CARBON DIOXIDE ANTFOCE OCEAN ACIDIFICATION LABORATORY AMD CEOS GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA > CASEY OCEAN > SOUTHERN OCEAN Sediment pigment analysis at Casey Station, 2014-2015 |
topic_facet |
oceans biota EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PIGMENTS CARBON DIOXIDE ANTFOCE OCEAN ACIDIFICATION LABORATORY AMD CEOS GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA > CASEY OCEAN > SOUTHERN OCEAN |
description |
Progress Code: completed Statement: These data are considered to be of high quality. Purpose This is an antFOCE dataset, collected in order to determine community composition from a pigment perspective. General description: The associated file contains sediment pigment data from the antFOCE project 4127. Units: all pigment data in ug/g, 0 = below detection limit of HPLC. Sample collection details: At the start and end of the antFOCE experiment, four sediment core samples were taken from inside and outside each chamber or open plot by divers. The top 1 cm of the cores was then removed and placed in the dark, first at -20ºC for 2 hours, then at -80ºC until analysis at the Australian Antarctic division. Pigment analysis Frozen samples were transported under liquid N2 to a freeze drier (Dynavac, model FD-5), in pre-chilled flasks with a small amount of liquid N2 added. Custom made plumbing fitted to the freeze drier enabled samples to be purged with N2 to prevent photo-oxidation up until solvent extraction. Prior to pigment extraction five 2 g stainless steel ball bearings were added to homogenise the freeze dried sediment. The samples were bead beaten for 1 minute (Biospec products). Subsamples (~0.05 g) were immediately transferred to cryotubes with 700 µl of dimethylformamide (DMF) for two hours. Samples were kept at -80ºC and under a safe light (IFORD 902) at all times. All pigment concentrations are standardised to sediment weight. Pigments were extracted with dimethylformamide (DMF 700 µl) over a two hour period at -20ºC. Zirconia beads, and 100 µl of Apo 8 and an internal standard were added to each sub-sample. After a two hour extraction, sub-samples were bead beaten for 20 seconds and then placed in a centrifuge with filter cartridge inserts for 14 minutes at 2500 rpm at -9ºC to separate the solvent from the sediment. The supernatant was transferred into to a vial and placed in a precooled rpHPLC autosampler. The rpHPLC system used is described in Hodgson et al. (1997). Pigment detection was at 435, ... |
format |
Dataset |
title |
Sediment pigment analysis at Casey Station, 2014-2015 |
title_short |
Sediment pigment analysis at Casey Station, 2014-2015 |
title_full |
Sediment pigment analysis at Casey Station, 2014-2015 |
title_fullStr |
Sediment pigment analysis at Casey Station, 2014-2015 |
title_full_unstemmed |
Sediment pigment analysis at Casey Station, 2014-2015 |
title_sort |
sediment pigment analysis at casey station, 2014-2015 |
publisher |
Australian Ocean Data Network |
url |
https://researchdata.edu.au/sediment-pigment-analysis-2014-2015/2818176 |
op_coverage |
Spatial: westlimit=96.32813; southlimit=-69.41124; eastlimit=120.9375; northlimit=-62.26792 Spatial: uplimit=15; downlimit=0 Temporal: From 2014-12-01 to 2015-03-01 |
long_lat |
ENVELOPE(110.528,110.528,-66.282,-66.282) ENVELOPE(166.083,166.083,-78.117,-78.117) |
geographic |
Antarctic Southern Ocean Casey Station Hodgson |
geographic_facet |
Antarctic Southern Ocean Casey Station Hodgson |
genre |
Antarc* Antarctic Antarctica Australian Antarctic Division Ocean acidification Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctica Australian Antarctic Division Ocean acidification Southern Ocean |
op_source |
AU/AADC > Australian Antarctic Data Centre, Australia |
op_relation |
https://researchdata.edu.au/sediment-pigment-analysis-2014-2015/2818176 AAS_4127_Sediment_pigment_data Dataset DOI |
_version_ |
1779309395823296512 |