Macromolecular data of diatoms exposed to Ocean Acidification - Mesocosm Experiments at Davis Station, Antarctica, 2014-2015
Progress Code: completed Purpose To understand Antarctic diatom species-specific macromolecular shifts in response to Ocean Acidification. Synchrotron based FTIR macromolecule profiles of 5 diatom species from the AAS_4026 ocean acidification project. Data represent the peak areas for wavenumbers re...
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ftands:oai:ands.org.au::2817843 2023-10-09T21:44:56+02:00 Macromolecular data of diatoms exposed to Ocean Acidification - Mesocosm Experiments at Davis Station, Antarctica, 2014-2015 Spatial: westlimit=69.40625; southlimit=-72.18491; eastlimit=86.6875; northlimit=-65.1282 Temporal: From 2014-11-20 to 2015-01-16 https://researchdata.edu.au/macromolecular-diatoms-exposed-2014-2015/2817843 unknown Australian Ocean Data Network https://researchdata.edu.au/macromolecular-diatoms-exposed-2014-2015/2817843 AAS_4026_FTIR Dataset DOI AU/AADC > Australian Antarctic Data Centre, Australia biota oceans EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > DIATOMS OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE SYNCHROTRON-BASED FOURIER TRANSFORM INFRARED MICROSPECTROSCOPY MESOCOSMS OCEAN ACIDIFICATION MACROMOLECULES FTIR SPECTROMETER > Fourier Transform Infrared Spectrometer LABORATORY AMD/AU AMD CEOS GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA > DAVIS STATION OCEAN > SOUTHERN OCEAN > PRYDZ BAY dataset ftands 2023-09-18T22:43:44Z Progress Code: completed Purpose To understand Antarctic diatom species-specific macromolecular shifts in response to Ocean Acidification. Synchrotron based FTIR macromolecule profiles of 5 diatom species from the AAS_4026 ocean acidification project. Data represent the peak areas for wavenumbers related to key macromolecules. For details on methods see Duncan et al. (2021) New Phytologist. Experimental design and mesocosm set up Mesocosm set up and conditions were as described previously (Deppeler et al., 2018; Hancock et al., 2018). Briefly, a near-shore, natural Antarctic microbial community was collected from an ice-free area among broken fast ice approximately 1km offshore from Davis Station, Antarctica (68° 35ʹ S, 77° 58ʹ E) on 19 November 2014. This community was incubated in 6 x 650L polyurethane tanks (mesocosms) across a gradient of fCO2 levels (343, 506, 634, 953, 1140 and 1641 μatm; denoted M1 – M6). These fCO2 levels corresponded to pH values ranging from 8.17 to 7.57. Temperature was maintained at 0.0 °C ± 0.5 °C and the mesocosms were stirred continuously by a central auger (15 r.p.m.) for gentle mixing and covered with an air-tight lid. Irradiance was initially kept low (0.8 ± 0.2 μmol photons m-2s-1), while cell physiology was left to acclimate to increasing fCO2 levels (over 5 days). When target fCO2 levels were reached in all six mesocosms, light was gradually increased (days 5-8) to 89 ± 16 μmol photons m-2s-1 on a 19 h:5 h light:dark cycle, to mimic current natural conditions. To generate the gradient in carbonate chemistry, filtered seawater saturated with CO2 was added to five of the mesocosms. Daily measurements were taken to monitor pH and dissolved inorganic carbon (DIC). For details of fCO2 manipulations, analytical procedures and calculations see Deppeler et al., (2018). Samples for physiological and macromolecular measurements in this study were taken on day 18, at the end of the incubation period (Deppeler et al., 2018). Cell volume Cell volume was determined for selected taxa ... Dataset Antarc* Antarctic Antarctica Ocean acidification Prydz Bay Southern Ocean Research Data Australia (Australian National Data Service - ANDS) Antarctic Southern Ocean Prydz Bay Davis Station ENVELOPE(77.968,77.968,-68.576,-68.576) Davis-Station ENVELOPE(77.968,77.968,-68.576,-68.576) |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
biota oceans EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > DIATOMS OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE SYNCHROTRON-BASED FOURIER TRANSFORM INFRARED MICROSPECTROSCOPY MESOCOSMS OCEAN ACIDIFICATION MACROMOLECULES FTIR SPECTROMETER > Fourier Transform Infrared Spectrometer LABORATORY AMD/AU AMD CEOS GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA > DAVIS STATION OCEAN > SOUTHERN OCEAN > PRYDZ BAY |
spellingShingle |
biota oceans EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > DIATOMS OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE SYNCHROTRON-BASED FOURIER TRANSFORM INFRARED MICROSPECTROSCOPY MESOCOSMS OCEAN ACIDIFICATION MACROMOLECULES FTIR SPECTROMETER > Fourier Transform Infrared Spectrometer LABORATORY AMD/AU AMD CEOS GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA > DAVIS STATION OCEAN > SOUTHERN OCEAN > PRYDZ BAY Macromolecular data of diatoms exposed to Ocean Acidification - Mesocosm Experiments at Davis Station, Antarctica, 2014-2015 |
topic_facet |
biota oceans EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > DIATOMS OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE SYNCHROTRON-BASED FOURIER TRANSFORM INFRARED MICROSPECTROSCOPY MESOCOSMS OCEAN ACIDIFICATION MACROMOLECULES FTIR SPECTROMETER > Fourier Transform Infrared Spectrometer LABORATORY AMD/AU AMD CEOS GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA > DAVIS STATION OCEAN > SOUTHERN OCEAN > PRYDZ BAY |
description |
Progress Code: completed Purpose To understand Antarctic diatom species-specific macromolecular shifts in response to Ocean Acidification. Synchrotron based FTIR macromolecule profiles of 5 diatom species from the AAS_4026 ocean acidification project. Data represent the peak areas for wavenumbers related to key macromolecules. For details on methods see Duncan et al. (2021) New Phytologist. Experimental design and mesocosm set up Mesocosm set up and conditions were as described previously (Deppeler et al., 2018; Hancock et al., 2018). Briefly, a near-shore, natural Antarctic microbial community was collected from an ice-free area among broken fast ice approximately 1km offshore from Davis Station, Antarctica (68° 35ʹ S, 77° 58ʹ E) on 19 November 2014. This community was incubated in 6 x 650L polyurethane tanks (mesocosms) across a gradient of fCO2 levels (343, 506, 634, 953, 1140 and 1641 μatm; denoted M1 – M6). These fCO2 levels corresponded to pH values ranging from 8.17 to 7.57. Temperature was maintained at 0.0 °C ± 0.5 °C and the mesocosms were stirred continuously by a central auger (15 r.p.m.) for gentle mixing and covered with an air-tight lid. Irradiance was initially kept low (0.8 ± 0.2 μmol photons m-2s-1), while cell physiology was left to acclimate to increasing fCO2 levels (over 5 days). When target fCO2 levels were reached in all six mesocosms, light was gradually increased (days 5-8) to 89 ± 16 μmol photons m-2s-1 on a 19 h:5 h light:dark cycle, to mimic current natural conditions. To generate the gradient in carbonate chemistry, filtered seawater saturated with CO2 was added to five of the mesocosms. Daily measurements were taken to monitor pH and dissolved inorganic carbon (DIC). For details of fCO2 manipulations, analytical procedures and calculations see Deppeler et al., (2018). Samples for physiological and macromolecular measurements in this study were taken on day 18, at the end of the incubation period (Deppeler et al., 2018). Cell volume Cell volume was determined for selected taxa ... |
format |
Dataset |
title |
Macromolecular data of diatoms exposed to Ocean Acidification - Mesocosm Experiments at Davis Station, Antarctica, 2014-2015 |
title_short |
Macromolecular data of diatoms exposed to Ocean Acidification - Mesocosm Experiments at Davis Station, Antarctica, 2014-2015 |
title_full |
Macromolecular data of diatoms exposed to Ocean Acidification - Mesocosm Experiments at Davis Station, Antarctica, 2014-2015 |
title_fullStr |
Macromolecular data of diatoms exposed to Ocean Acidification - Mesocosm Experiments at Davis Station, Antarctica, 2014-2015 |
title_full_unstemmed |
Macromolecular data of diatoms exposed to Ocean Acidification - Mesocosm Experiments at Davis Station, Antarctica, 2014-2015 |
title_sort |
macromolecular data of diatoms exposed to ocean acidification - mesocosm experiments at davis station, antarctica, 2014-2015 |
publisher |
Australian Ocean Data Network |
url |
https://researchdata.edu.au/macromolecular-diatoms-exposed-2014-2015/2817843 |
op_coverage |
Spatial: westlimit=69.40625; southlimit=-72.18491; eastlimit=86.6875; northlimit=-65.1282 Temporal: From 2014-11-20 to 2015-01-16 |
long_lat |
ENVELOPE(77.968,77.968,-68.576,-68.576) ENVELOPE(77.968,77.968,-68.576,-68.576) |
geographic |
Antarctic Southern Ocean Prydz Bay Davis Station Davis-Station |
geographic_facet |
Antarctic Southern Ocean Prydz Bay Davis Station Davis-Station |
genre |
Antarc* Antarctic Antarctica Ocean acidification Prydz Bay Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctica Ocean acidification Prydz Bay Southern Ocean |
op_source |
AU/AADC > Australian Antarctic Data Centre, Australia |
op_relation |
https://researchdata.edu.au/macromolecular-diatoms-exposed-2014-2015/2817843 AAS_4026_FTIR Dataset DOI |
_version_ |
1779314661072568320 |