Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates
Progress Code: completed Statement: See the referenced paper for more information. Purpose Samples were collected to determine the affect that sample age, collection substrate, breeding stage and incubation duration had on the DNA amplification success and the proportion of food DNA. Using these dat...
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ftands:oai:ands.org.au::2816817 2023-10-09T21:56:03+02:00 Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates Spatial: westlimit=144.65; southlimit=-40.383333; eastlimit=144.65; northlimit=-40.383333 Temporal: From 2014-03-28 to 2015-03-31 https://researchdata.edu.au/optimised-scat-collection-metabarcoding-vertebrates/2816817 unknown Australian Ocean Data Network https://researchdata.edu.au/optimised-scat-collection-metabarcoding-vertebrates/2816817 AAS_4014_McInnes_et_al_2016 Dataset DOI AU/AADC > Australian Antarctic Data Centre, Australia biota oceans EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > BIRDS > ALBATROSSES/PETRELS AND ALLIES DNA METABARCODING MISEQ GENOME SEQUENCING SHY ALBATROSS DIET THALASSARCHE CAUTA ALBATROSS ISLAND AMD/AU AMD CEOS GEOGRAPHIC REGION > POLAR OCEAN > SOUTHERN OCEAN CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA > Tasmania dataset ftands 2023-09-18T22:42:40Z Progress Code: completed Statement: See the referenced paper for more information. Purpose Samples were collected to determine the affect that sample age, collection substrate, breeding stage and incubation duration had on the DNA amplification success and the proportion of food DNA. Using these data, optimised scat collection protocols were developed. These spreadsheets provide the sequences counts for the DNA groups found in the scats of Shy Albatross at Albatross Island, Tasmania, Australia. Samples were collected during three stages of the breeding season: Incubation (Late September 2014), Brood (December 2014) and Chick-rearing (March 2014 and 2015). Scat samples were collected from breeding birds, chicks and non-breeders. 'Breeders' were identified as individuals incubating an egg or attending a chick, whereas 'non-breeders' were usually pairs that had reoccupied the colony and were building new practice nests with no chick present. Non-breeders in the colony include immature birds that have not yet bred and mature birds of breeding age that did not breed in a particular season (e.g. no partner or insufficient body condition) Details of the molecular methods and synthesis of this data can be found in: McInnes, J.C., Alderman, R., Deagle, B.E. Lea, M-A., Raymond, B. and Jarman, S.N. 2016. Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates. Methods in Ecology and Evolution. Dataset Southern Ocean Research Data Australia (Australian National Data Service - ANDS) Southern Ocean New Zealand Albatross Island ENVELOPE(-37.332,-37.332,-54.020,-54.020) |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
biota oceans EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > BIRDS > ALBATROSSES/PETRELS AND ALLIES DNA METABARCODING MISEQ GENOME SEQUENCING SHY ALBATROSS DIET THALASSARCHE CAUTA ALBATROSS ISLAND AMD/AU AMD CEOS GEOGRAPHIC REGION > POLAR OCEAN > SOUTHERN OCEAN CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA > Tasmania |
spellingShingle |
biota oceans EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > BIRDS > ALBATROSSES/PETRELS AND ALLIES DNA METABARCODING MISEQ GENOME SEQUENCING SHY ALBATROSS DIET THALASSARCHE CAUTA ALBATROSS ISLAND AMD/AU AMD CEOS GEOGRAPHIC REGION > POLAR OCEAN > SOUTHERN OCEAN CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA > Tasmania Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates |
topic_facet |
biota oceans EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > BIRDS > ALBATROSSES/PETRELS AND ALLIES DNA METABARCODING MISEQ GENOME SEQUENCING SHY ALBATROSS DIET THALASSARCHE CAUTA ALBATROSS ISLAND AMD/AU AMD CEOS GEOGRAPHIC REGION > POLAR OCEAN > SOUTHERN OCEAN CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA > Tasmania |
description |
Progress Code: completed Statement: See the referenced paper for more information. Purpose Samples were collected to determine the affect that sample age, collection substrate, breeding stage and incubation duration had on the DNA amplification success and the proportion of food DNA. Using these data, optimised scat collection protocols were developed. These spreadsheets provide the sequences counts for the DNA groups found in the scats of Shy Albatross at Albatross Island, Tasmania, Australia. Samples were collected during three stages of the breeding season: Incubation (Late September 2014), Brood (December 2014) and Chick-rearing (March 2014 and 2015). Scat samples were collected from breeding birds, chicks and non-breeders. 'Breeders' were identified as individuals incubating an egg or attending a chick, whereas 'non-breeders' were usually pairs that had reoccupied the colony and were building new practice nests with no chick present. Non-breeders in the colony include immature birds that have not yet bred and mature birds of breeding age that did not breed in a particular season (e.g. no partner or insufficient body condition) Details of the molecular methods and synthesis of this data can be found in: McInnes, J.C., Alderman, R., Deagle, B.E. Lea, M-A., Raymond, B. and Jarman, S.N. 2016. Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates. Methods in Ecology and Evolution. |
format |
Dataset |
title |
Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates |
title_short |
Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates |
title_full |
Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates |
title_fullStr |
Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates |
title_full_unstemmed |
Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates |
title_sort |
optimised scat collection protocols for dietary dna metabarcoding in vertebrates |
publisher |
Australian Ocean Data Network |
url |
https://researchdata.edu.au/optimised-scat-collection-metabarcoding-vertebrates/2816817 |
op_coverage |
Spatial: westlimit=144.65; southlimit=-40.383333; eastlimit=144.65; northlimit=-40.383333 Temporal: From 2014-03-28 to 2015-03-31 |
long_lat |
ENVELOPE(-37.332,-37.332,-54.020,-54.020) |
geographic |
Southern Ocean New Zealand Albatross Island |
geographic_facet |
Southern Ocean New Zealand Albatross Island |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_source |
AU/AADC > Australian Antarctic Data Centre, Australia |
op_relation |
https://researchdata.edu.au/optimised-scat-collection-metabarcoding-vertebrates/2816817 AAS_4014_McInnes_et_al_2016 Dataset DOI |
_version_ |
1779320453292097536 |