Resilience of Antarctic marine benthic invertebrates and the ecological consequences of environmental change - Amphipod Data

• Format: Dataset
• Language: unknown
• Published: Australian Ocean Data Network
• Subjects: biota; oceans; EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS > AMPHIPODS; ADS > Automated DNA Sequencer; R/V POLARSTERN; R/V AA > R/V Aurora Australis; AMD/AU; CEOS; AMD; OCEAN > SOUTHERN OCEAN; CONTINENT > ANTARCTICA; GEOGRAPHIC REGION > POLAR; Antarctic; Southern Ocean; The Antarctic; Giganteus; Antarc*; Antarctica; aurora australis
• Online Access: https://researchdata.edu.au/resilience-antarctic-marine-amphipod-data/2816742

Progress Code: completed Statement: Some problems with the quality of sequences obtained for the ITS2 gene region: this is explained in the associated publication. Original datasheets were reformatted to fit IPT Biodiversity.AQ standard. The new datasheet "Amphipods.csv" provides the dataset ID, verbatim latitude and verbatim longitude that were converted to decimal latitude and longitude, also provided. Taxonomical organisation to the lowest taxonomical rank that could be determined, after matched in WoRMS (World Register of Marine Species). The associated sequences for identified species and the associated reference. Overview The aim of the project was to assess the genetic connectivity of benthic amphipods (crustaceans) on a circumantarctic scale. Two sibling amphipod species were chosen as the subjects for this study: Eusirus perdentatus and Eusirus giganteus. Samples of both species were collected (or donated by other institutions) from five broad regions of the Antarctic coast (see 'Sample location information' worksheet). The dataset we generated represents DNA sequences we obtained from these amphipods. Each amphipod was sequenced for three gene regions - these were cytochrome oxidase subunit I (COI), internal transcribed spacer 2 (ITS2) and cytochrome b (CytB). Each DNA sequence generated has been deposited on the publicly-accessible GenBank website (www.ncbi.nlm.nih.gov/genbank/) and therefore has its own accession number (which can be typed into the GenBank search bar to access the actual DNA sequence in .fasta format). The attached spreadsheet provides details on the location, depth and date of each amphipod sample collected, the preliminary species ID for each amphipod*, and the resultant DNA sequences corresponding to each of the three gene regions amplified (these are provided as Genbank accession numbers). *Results of this project have actually highlighted that Eusirus perdentatus and Eusirus giganteus almost certainly contain several extra cryptic species, therefore these ID's are likely to ...