SWATT leaf d13C

• Other Authors: Byrne, Margaret (coAuthor), Caddy-Retalic, Stefan (author), Curtin University (hasAssociationWith), Department of Biodiversity, Conservation and Attractions, Western Australian Government (hasAssociationWith), McInerney, Francesca (coAuthor), Nielson, Kristine (pointOfContact), Nielson, Kristine (coAuthor), Terrestrial Ecosystem Research Network (distributor), Terrestrial Ecosystem Research Network (publisher), Terrestrial Ecosystem Research Network (pointOfContact), University of Adelaide (hasAssociationWith), van Leeuwen, Stephen (coAuthor)
• Format: Dataset
• Language: unknown
• Published: Terrestrial Ecosystem Research Network
• Subjects: biota; CARBON ISOTOPE; EARTH SCIENCE; CLIMATE INDICATORS; PALEOCLIMATE INDICATORS; BIOLOGICAL RECORDS; TREE RINGS; ISOTOPIC ANALYSIS; NITROGEN ISOTOPES; ICE CORE RECORDS; ISOTOPES; STABLE ISOTOPES; DROUGHTS; HUMAN DIMENSIONS; NATURAL HAZARDS; TERRESTRIAL ECOSYSTEMS; BIOSPHERE; Climate change science; Ecological impacts of climate change and ecological adaptation; field species name (Unitless); Unitless; leaf carbon content (Percent); Percent; mean leaf nitrogen content (Percent); maximum temperature (Degree Celsius); Degree Celsius; elevation of site (Metre); Metre; growth form (Unitless); leaf Delta13C (Parts per thousand); Parts per thousand; leaf Delta15N (Parts per thousand); 250 km - < 500 km or approximately 2.5 degrees - < 5.0 degrees; irregular; Arid ecology; Ecosystem Modelling; Long-Term Community Monitoring; Long-Term Species Monitoring; leaf isotopes; Climate; Landscape Type; Native Vegetation; Vegetation Structure; New Zealand; Murchison; ice core
• Online Access: https://researchdata.edu.au/swatt-leaf-d13c/2048042; https://geonetwork.tern.org.au/geonetwork/srv/eng/catalog.search#/metadata/6564a202-6665-4a48-b72d-2c3b10f0fd48

Isotope ratio mass spectrometry (IRMS): Leaf tissue samples from were dried in synthetic gauze bags on silica gel. Samples from species that occurred at more than seven sites (see Table SWATT_climate2.csv for locations) were selected, with the exception of one species (Grevillea hookeriana), which is only present at six sites. Leaves were ground using a ball mill (Retsch MM400 fitted with a Qiagen TissueLyser 24 adapter set). Leaves that did not grind within 3 minutes in the TissueLyzer (for example, Allocasuarina spinosissima) were ground in a 5 mL stainless steel grinding jar. For carbon and nitrogen isotope analysis, 2-2.5 mg of ground material was weighed to six significant figures into a tin capsule using an A&D BM-22 microbalance. Technical replicates for 13% of samples were run to correct for drift. Samples were combusted at 1000°C in an Elemental Analyser (EuroVector EuroEA 3000) in line with a continuous flow isotope ratio mass spectrometer (Nu Instruments Nu Horizon, University of Adelaide). Pure glycine, glutamic acid and triphenylamine (all calibrated to international C & N standards) were used as standards. A standard error of 0.07‰ was achieved. Two-point drift and size corrections based on glycine and glutamic acid standards were undertaken. Growth forms were attributed to species based on the growth forms described in the National Vegetation Attribute Manual (ESCAVI, 2003) with grasses and forbs further divided in to annual and perennial groups. Sub-specific determinations were ignored. Climate data were extracted from BIOCLIM layers modelled at 9 second resolution extracted from ANUCLIM 6.1 (Xu & Hutchinson, 2013). Credit We at TERN acknowledge the Traditional Owners and Custodians throughout Australia, New Zealand and all nations. We honour their profound connections to land, water, biodiversity and culture and pay our respects to their Elders past, present and emerging. Purpose This project was established to investigate the leaf stable carbon isotope response of terrestrial ...