Table S2 Iron limitation drives the globally extreme Fluorescence/Chlorophyll ratios of the Southern Ocean [Paper # 2021GL097616]
Maintenance and Update Frequency: none-planned Statement: Southern Ocean phytoplankton cultures were grown in the artificial seawater medium Aquil enriched with 10 μmol L-1 phosphate, 100 μmol L-1 silicate, 300 μmol L-1 nitrate, and filter-sterilized (0.2 μm Gelman Acrodisc PF) trace metal and vitam...
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Online Access: | https://researchdata.edu.au/table-s2-iron-paper-2021gl097616/1894725 |
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ftands:oai:ands.org.au::1894725 2023-10-25T01:29:28+02:00 Table S2 Iron limitation drives the globally extreme Fluorescence/Chlorophyll ratios of the Southern Ocean [Paper # 2021GL097616] IMAS Data Manager (pointOfContact) Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) (hasAssociationWith) Strzepek, Robert (pointOfContact) Strzepek, Robert (hasPrincipalInvestigator) Strzepek, Robert, Dr (pointOfContact) Strzepek, Robert, Dr (hasPrincipalInvestigator) University of Tasmania (hasAssociationWith) Spatial: westlimit=147.17578124999997; southlimit=-43.01704213347566; eastlimit=147.48339843750003; northlimit=-42.825401675346875 Spatial: westlimit=170.42285156249997; southlimit=-45.979575693783616; eastlimit=170.57666015625006; northlimit=-45.80998632748884 Temporal: From 2005-05-01 to 2021-11-15 https://researchdata.edu.au/table-s2-iron-paper-2021gl097616/1894725 unknown Australian Ocean Data Network https://researchdata.edu.au/table-s2-iron-paper-2021gl097616/1894725 4b9ea473-c601-48da-abb3-2eded2e8e222 University of Tasmania oceans Phaeocystis antarctica Proboscia inermis Eucampia antarctica Chaetoceros neogracilis Chaetoceros flexuosus EARTH SCIENCE | BIOSPHERE | ECOLOGICAL DYNAMICS | ECOSYSTEM FUNCTIONS | PHOTOSYNTHESIS EARTH SCIENCE | OCEANS | OCEAN OPTICS | FLUORESCENCE EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | PLANKTON | PHYTOPLANKTON Biological Oceanography EARTH SCIENCES OCEANOGRAPHY Chemical Oceanography dataset ftands 2023-09-25T23:47:25Z Maintenance and Update Frequency: none-planned Statement: Southern Ocean phytoplankton cultures were grown in the artificial seawater medium Aquil enriched with 10 μmol L-1 phosphate, 100 μmol L-1 silicate, 300 μmol L-1 nitrate, and filter-sterilized (0.2 μm Gelman Acrodisc PF) trace metal and vitamin solutions as described in Strzepek et al. (2011). Trace metal concentrations were buffered with the synthetic organic chelator, ethylene-diamine-tetra-acetic acid (EDTA). Iron limiting treatments for Phaeocystis antarctica, and the diatoms Proboscia inermis and Eucampia antarctica employed the siderophore desferrioxamine B (DFB) to control the degree of Fe limitation. Iron replete and iron limiting media for these three species have been described previously (Strzepek et al. 2011). The diatoms Fragilariopsis cylindrus, Chaetoceros neogracilis and Chaetoceros flexuosus were grown in Aquil medium containing 100 μmol L-1 of EDTA. Iron replete medium contained 567 nmol L-1 Fe; iron limiting medium contained 1.5 nmol L-1 Fe for C. neogracilis and C. flexuosus, and 16.7 nmol L-1 Fe for F. cylindrus. Cultures were grown in triplicate, maintained in exponential phase by dilution, and allowed to acclimate to growth conditions for at least two transfers (14–16 cell divisions) before data collection. All cultures were grown at 2.5-3 °C under continuous PAR using cool-white fluorescent bulbs. P. antarctica was grown at 6 PAR levels (10, 20, 30, 60, 100, and 400 μmol quanta m-2 s-1) while the diatoms were grown at 30-35 μmol photons m-2 s-1 . PAR was measured with a 4π quantum meter (model QSL-2101, Biospherical Instruments). In vivo Chl fluorescence (F) of dark-acclimated (30 min) cultures was determined using a Turner Designs model 10-AU fluorometer. To measure cellular Chl concentrations, 25 mL of culture were filtered (< 75 mm Hg) onto glass fibre filters (GF/F; Whatman) immediately following in vivo Chl fluorescence measurements. The filters were extracted in 90% acetone in the dark for 18 h at -20 °C prior to ... Dataset Antarc* Antarctica Southern Ocean Research Data Australia (Australian National Data Service - ANDS) Southern Ocean |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
oceans Phaeocystis antarctica Proboscia inermis Eucampia antarctica Chaetoceros neogracilis Chaetoceros flexuosus EARTH SCIENCE | BIOSPHERE | ECOLOGICAL DYNAMICS | ECOSYSTEM FUNCTIONS | PHOTOSYNTHESIS EARTH SCIENCE | OCEANS | OCEAN OPTICS | FLUORESCENCE EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | PLANKTON | PHYTOPLANKTON Biological Oceanography EARTH SCIENCES OCEANOGRAPHY Chemical Oceanography |
spellingShingle |
oceans Phaeocystis antarctica Proboscia inermis Eucampia antarctica Chaetoceros neogracilis Chaetoceros flexuosus EARTH SCIENCE | BIOSPHERE | ECOLOGICAL DYNAMICS | ECOSYSTEM FUNCTIONS | PHOTOSYNTHESIS EARTH SCIENCE | OCEANS | OCEAN OPTICS | FLUORESCENCE EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | PLANKTON | PHYTOPLANKTON Biological Oceanography EARTH SCIENCES OCEANOGRAPHY Chemical Oceanography Table S2 Iron limitation drives the globally extreme Fluorescence/Chlorophyll ratios of the Southern Ocean [Paper # 2021GL097616] |
topic_facet |
oceans Phaeocystis antarctica Proboscia inermis Eucampia antarctica Chaetoceros neogracilis Chaetoceros flexuosus EARTH SCIENCE | BIOSPHERE | ECOLOGICAL DYNAMICS | ECOSYSTEM FUNCTIONS | PHOTOSYNTHESIS EARTH SCIENCE | OCEANS | OCEAN OPTICS | FLUORESCENCE EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | PROTISTS | PLANKTON | PHYTOPLANKTON Biological Oceanography EARTH SCIENCES OCEANOGRAPHY Chemical Oceanography |
description |
Maintenance and Update Frequency: none-planned Statement: Southern Ocean phytoplankton cultures were grown in the artificial seawater medium Aquil enriched with 10 μmol L-1 phosphate, 100 μmol L-1 silicate, 300 μmol L-1 nitrate, and filter-sterilized (0.2 μm Gelman Acrodisc PF) trace metal and vitamin solutions as described in Strzepek et al. (2011). Trace metal concentrations were buffered with the synthetic organic chelator, ethylene-diamine-tetra-acetic acid (EDTA). Iron limiting treatments for Phaeocystis antarctica, and the diatoms Proboscia inermis and Eucampia antarctica employed the siderophore desferrioxamine B (DFB) to control the degree of Fe limitation. Iron replete and iron limiting media for these three species have been described previously (Strzepek et al. 2011). The diatoms Fragilariopsis cylindrus, Chaetoceros neogracilis and Chaetoceros flexuosus were grown in Aquil medium containing 100 μmol L-1 of EDTA. Iron replete medium contained 567 nmol L-1 Fe; iron limiting medium contained 1.5 nmol L-1 Fe for C. neogracilis and C. flexuosus, and 16.7 nmol L-1 Fe for F. cylindrus. Cultures were grown in triplicate, maintained in exponential phase by dilution, and allowed to acclimate to growth conditions for at least two transfers (14–16 cell divisions) before data collection. All cultures were grown at 2.5-3 °C under continuous PAR using cool-white fluorescent bulbs. P. antarctica was grown at 6 PAR levels (10, 20, 30, 60, 100, and 400 μmol quanta m-2 s-1) while the diatoms were grown at 30-35 μmol photons m-2 s-1 . PAR was measured with a 4π quantum meter (model QSL-2101, Biospherical Instruments). In vivo Chl fluorescence (F) of dark-acclimated (30 min) cultures was determined using a Turner Designs model 10-AU fluorometer. To measure cellular Chl concentrations, 25 mL of culture were filtered (< 75 mm Hg) onto glass fibre filters (GF/F; Whatman) immediately following in vivo Chl fluorescence measurements. The filters were extracted in 90% acetone in the dark for 18 h at -20 °C prior to ... |
author2 |
IMAS Data Manager (pointOfContact) Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) (hasAssociationWith) Strzepek, Robert (pointOfContact) Strzepek, Robert (hasPrincipalInvestigator) Strzepek, Robert, Dr (pointOfContact) Strzepek, Robert, Dr (hasPrincipalInvestigator) University of Tasmania (hasAssociationWith) |
format |
Dataset |
title |
Table S2 Iron limitation drives the globally extreme Fluorescence/Chlorophyll ratios of the Southern Ocean [Paper # 2021GL097616] |
title_short |
Table S2 Iron limitation drives the globally extreme Fluorescence/Chlorophyll ratios of the Southern Ocean [Paper # 2021GL097616] |
title_full |
Table S2 Iron limitation drives the globally extreme Fluorescence/Chlorophyll ratios of the Southern Ocean [Paper # 2021GL097616] |
title_fullStr |
Table S2 Iron limitation drives the globally extreme Fluorescence/Chlorophyll ratios of the Southern Ocean [Paper # 2021GL097616] |
title_full_unstemmed |
Table S2 Iron limitation drives the globally extreme Fluorescence/Chlorophyll ratios of the Southern Ocean [Paper # 2021GL097616] |
title_sort |
table s2 iron limitation drives the globally extreme fluorescence/chlorophyll ratios of the southern ocean [paper # 2021gl097616] |
publisher |
Australian Ocean Data Network |
url |
https://researchdata.edu.au/table-s2-iron-paper-2021gl097616/1894725 |
op_coverage |
Spatial: westlimit=147.17578124999997; southlimit=-43.01704213347566; eastlimit=147.48339843750003; northlimit=-42.825401675346875 Spatial: westlimit=170.42285156249997; southlimit=-45.979575693783616; eastlimit=170.57666015625006; northlimit=-45.80998632748884 Temporal: From 2005-05-01 to 2021-11-15 |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Antarc* Antarctica Southern Ocean |
genre_facet |
Antarc* Antarctica Southern Ocean |
op_source |
University of Tasmania |
op_relation |
https://researchdata.edu.au/table-s2-iron-paper-2021gl097616/1894725 4b9ea473-c601-48da-abb3-2eded2e8e222 |
_version_ |
1780732815234564096 |