Trophic markers of marine predators in the Southern Ocean
Maintenance and Update Frequency: irregular Statement: Common methodologies: Blubber analysis of southern elephant seals The biopsy site was located by measuring 5–7 cm laterally from a site on the posterior dorsal surface of the seal (Best et al. 2003). A 2 cm x 2 cm square area was shaved and disi...
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University of Tasmania, Australia
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ftands:oai:ands.org.au::1728600 2024-09-15T17:43:53+00:00 Trophic markers of marine predators in the Southern Ocean Hindell, Mark (hasPrincipalInvestigator) Hindell, Mark, Dr (hasPrincipalInvestigator) Hindell, Mark, Prof. (hasPrincipalInvestigator) IMAS Data Manager (pointOfContact) Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) (hasAssociationWith) School of Zoology, University of Tasmania (UTAS) (hasAssociationWith) Spatial: westlimit=-180; southlimit=-85; eastlimit=180; northlimit=-60 Spatial: uplimit=2000; downlimit=0 https://researchdata.edu.au/trophic-markers-marine-southern-ocean/1728600 unknown University of Tasmania, Australia https://researchdata.edu.au/trophic-markers-marine-southern-ocean/1728600 7f918fa0-85ab-11dc-b3c2-00188b4c0af8 Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) biota Oceans | Marine Biology | Marine Mammals Oceans | Marine Biology | Marine Birds 41 131002 41 136004 41 136002 41 131004 40 001004 - Royal penguin and macaroni penguin 40 041038 40 041047 Arctocephalus gazella Mirounga leonina Leptonychotes weddelli Arctocephalus tropicalis Eudyptes chrysolophus Eudyptes chrysolophus schlegeli Puffinus carneipes Puffinus tenuirostris Antarctic fur-seal Southern elephant seal Weddell seal Subantarctic fur-seal Macaroni penguin Royal penguin Flesh-footed shearwater Short-tailed shearwater Marine and Estuarine Ecology (incl. Marine Ichthyology) BIOLOGICAL SCIENCES ECOLOGY Wildlife and Habitat Management ENVIRONMENTAL SCIENCES ENVIRONMENTAL SCIENCE AND MANAGEMENT Physical Oceanography EARTH SCIENCES OCEANOGRAPHY dataset ftands 2024-08-06T01:58:58Z Maintenance and Update Frequency: irregular Statement: Common methodologies: Blubber analysis of southern elephant seals The biopsy site was located by measuring 5–7 cm laterally from a site on the posterior dorsal surface of the seal (Best et al. 2003). A 2 cm x 2 cm square area was shaved and disinfected with an alcohol swab. A 1 cm anterior–posterior line was cut through the skin, and the biopsy corer (6 mm in diameter) was inserted into this incision. Biopsies contained ‘whole’ cores of blubber from the skin to the muscle layer. No suturing of the incision was required. Each core was placed into a vial containing a solvent mixture of 2 : 1 v/v chloroform and methanol, and 0.05% by weight of the anti-oxidizing agent, butylated hydroxytoluene. Samples were maintained at 220 °C until lipid analysis. Lipids were extracted following Best et al. (2003). Briefly, we used a modified version of the Bligh & Dyer (1959) one-phase methanol–chloroform–water extraction (ratio modified to 2 : 1 : 0.8 by volume). Chloroform and saline water were added to separate the phases following overnight extraction (final solvent ratio of 1 : 1 : 0.9 by volume). Solvents were removed using rotary evaporation (40 °C), and the total lipid (TL) extracted (greater than 98%) was dissolved in chloroform and an aliquot treated with methanol–hydrochloric acid–chloroform (10 : 1 : 1 v/v/v; 80 °C; 2 h). TL samples were vortexed two to four times during that time to maximize conversion to FA methyl esters (FAME). The FAME were extracted three times into hexane–chloroform (4 : 1 v/v, 3 ml ´ 1.8 ml; 11 ml water) and subjected to gas chromatographic analyses using a Hewlett Packard 5890A GC (Avondale, PA, USA). Peaks were quantified with Waters Millennium software (Milford, MA, USA). Individual components were identified by comparing retention-time data with authentic and laboratory standards. Integrated chromatograms were normalized by expressing the FA components as percentages of the total FA. FA components that occurred at less than 0.5% ... Dataset Antarc* Antarctic Antarctic Fur Seal Arctocephalus gazella Elephant Seal Elephant Seals Eudyptes chrysolophus Leptonychotes weddelli Macaroni penguin Mirounga leonina Puffinus tenuirostris Southern Elephant Seal Southern Elephant Seals Southern Ocean Weddell Seal Research Data Australia (Australian National Data Service - ANDS) |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
biota Oceans | Marine Biology | Marine Mammals Oceans | Marine Biology | Marine Birds 41 131002 41 136004 41 136002 41 131004 40 001004 - Royal penguin and macaroni penguin 40 041038 40 041047 Arctocephalus gazella Mirounga leonina Leptonychotes weddelli Arctocephalus tropicalis Eudyptes chrysolophus Eudyptes chrysolophus schlegeli Puffinus carneipes Puffinus tenuirostris Antarctic fur-seal Southern elephant seal Weddell seal Subantarctic fur-seal Macaroni penguin Royal penguin Flesh-footed shearwater Short-tailed shearwater Marine and Estuarine Ecology (incl. Marine Ichthyology) BIOLOGICAL SCIENCES ECOLOGY Wildlife and Habitat Management ENVIRONMENTAL SCIENCES ENVIRONMENTAL SCIENCE AND MANAGEMENT Physical Oceanography EARTH SCIENCES OCEANOGRAPHY |
spellingShingle |
biota Oceans | Marine Biology | Marine Mammals Oceans | Marine Biology | Marine Birds 41 131002 41 136004 41 136002 41 131004 40 001004 - Royal penguin and macaroni penguin 40 041038 40 041047 Arctocephalus gazella Mirounga leonina Leptonychotes weddelli Arctocephalus tropicalis Eudyptes chrysolophus Eudyptes chrysolophus schlegeli Puffinus carneipes Puffinus tenuirostris Antarctic fur-seal Southern elephant seal Weddell seal Subantarctic fur-seal Macaroni penguin Royal penguin Flesh-footed shearwater Short-tailed shearwater Marine and Estuarine Ecology (incl. Marine Ichthyology) BIOLOGICAL SCIENCES ECOLOGY Wildlife and Habitat Management ENVIRONMENTAL SCIENCES ENVIRONMENTAL SCIENCE AND MANAGEMENT Physical Oceanography EARTH SCIENCES OCEANOGRAPHY Trophic markers of marine predators in the Southern Ocean |
topic_facet |
biota Oceans | Marine Biology | Marine Mammals Oceans | Marine Biology | Marine Birds 41 131002 41 136004 41 136002 41 131004 40 001004 - Royal penguin and macaroni penguin 40 041038 40 041047 Arctocephalus gazella Mirounga leonina Leptonychotes weddelli Arctocephalus tropicalis Eudyptes chrysolophus Eudyptes chrysolophus schlegeli Puffinus carneipes Puffinus tenuirostris Antarctic fur-seal Southern elephant seal Weddell seal Subantarctic fur-seal Macaroni penguin Royal penguin Flesh-footed shearwater Short-tailed shearwater Marine and Estuarine Ecology (incl. Marine Ichthyology) BIOLOGICAL SCIENCES ECOLOGY Wildlife and Habitat Management ENVIRONMENTAL SCIENCES ENVIRONMENTAL SCIENCE AND MANAGEMENT Physical Oceanography EARTH SCIENCES OCEANOGRAPHY |
description |
Maintenance and Update Frequency: irregular Statement: Common methodologies: Blubber analysis of southern elephant seals The biopsy site was located by measuring 5–7 cm laterally from a site on the posterior dorsal surface of the seal (Best et al. 2003). A 2 cm x 2 cm square area was shaved and disinfected with an alcohol swab. A 1 cm anterior–posterior line was cut through the skin, and the biopsy corer (6 mm in diameter) was inserted into this incision. Biopsies contained ‘whole’ cores of blubber from the skin to the muscle layer. No suturing of the incision was required. Each core was placed into a vial containing a solvent mixture of 2 : 1 v/v chloroform and methanol, and 0.05% by weight of the anti-oxidizing agent, butylated hydroxytoluene. Samples were maintained at 220 °C until lipid analysis. Lipids were extracted following Best et al. (2003). Briefly, we used a modified version of the Bligh & Dyer (1959) one-phase methanol–chloroform–water extraction (ratio modified to 2 : 1 : 0.8 by volume). Chloroform and saline water were added to separate the phases following overnight extraction (final solvent ratio of 1 : 1 : 0.9 by volume). Solvents were removed using rotary evaporation (40 °C), and the total lipid (TL) extracted (greater than 98%) was dissolved in chloroform and an aliquot treated with methanol–hydrochloric acid–chloroform (10 : 1 : 1 v/v/v; 80 °C; 2 h). TL samples were vortexed two to four times during that time to maximize conversion to FA methyl esters (FAME). The FAME were extracted three times into hexane–chloroform (4 : 1 v/v, 3 ml ´ 1.8 ml; 11 ml water) and subjected to gas chromatographic analyses using a Hewlett Packard 5890A GC (Avondale, PA, USA). Peaks were quantified with Waters Millennium software (Milford, MA, USA). Individual components were identified by comparing retention-time data with authentic and laboratory standards. Integrated chromatograms were normalized by expressing the FA components as percentages of the total FA. FA components that occurred at less than 0.5% ... |
author2 |
Hindell, Mark (hasPrincipalInvestigator) Hindell, Mark, Dr (hasPrincipalInvestigator) Hindell, Mark, Prof. (hasPrincipalInvestigator) IMAS Data Manager (pointOfContact) Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) (hasAssociationWith) School of Zoology, University of Tasmania (UTAS) (hasAssociationWith) |
format |
Dataset |
title |
Trophic markers of marine predators in the Southern Ocean |
title_short |
Trophic markers of marine predators in the Southern Ocean |
title_full |
Trophic markers of marine predators in the Southern Ocean |
title_fullStr |
Trophic markers of marine predators in the Southern Ocean |
title_full_unstemmed |
Trophic markers of marine predators in the Southern Ocean |
title_sort |
trophic markers of marine predators in the southern ocean |
publisher |
University of Tasmania, Australia |
url |
https://researchdata.edu.au/trophic-markers-marine-southern-ocean/1728600 |
op_coverage |
Spatial: westlimit=-180; southlimit=-85; eastlimit=180; northlimit=-60 Spatial: uplimit=2000; downlimit=0 |
genre |
Antarc* Antarctic Antarctic Fur Seal Arctocephalus gazella Elephant Seal Elephant Seals Eudyptes chrysolophus Leptonychotes weddelli Macaroni penguin Mirounga leonina Puffinus tenuirostris Southern Elephant Seal Southern Elephant Seals Southern Ocean Weddell Seal |
genre_facet |
Antarc* Antarctic Antarctic Fur Seal Arctocephalus gazella Elephant Seal Elephant Seals Eudyptes chrysolophus Leptonychotes weddelli Macaroni penguin Mirounga leonina Puffinus tenuirostris Southern Elephant Seal Southern Elephant Seals Southern Ocean Weddell Seal |
op_source |
Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) |
op_relation |
https://researchdata.edu.au/trophic-markers-marine-southern-ocean/1728600 7f918fa0-85ab-11dc-b3c2-00188b4c0af8 |
_version_ |
1810491105980973056 |