Voyage 4 of the Aurora Australis, 2018 environmental DNA samples analysed with group specific metabarcoding markers
In March 2018, 23 environmental DNA (eDNA) samples (2 L of filtered seawater) were collected between Hobart, Tasmania and subantarctic Macquarie Island. These samples were processed using six different genetic metabarcoding markers targeting different taxonomic groups within the metazoan clade: A br...
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Online Access: | https://researchdata.edu.au/voyage-4-aurora-metabarcoding-markers/1675086 https://doi.org/10.26179/0byh-s246 https://data.aad.gov.au/metadata/records/AAS_4556_group_specific_eDNA_V4_2018 http://nla.gov.au/nla.party-617536 |
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ftands:oai:ands.org.au::1675086 2023-05-15T15:33:48+02:00 Voyage 4 of the Aurora Australis, 2018 environmental DNA samples analysed with group specific metabarcoding markers SUTER, LEONIE (hasPrincipalInvestigator) SUTER, LEONIE (processor) POLANOWSKI, ANDREA (hasPrincipalInvestigator) DEAGLE, BRUCE (hasPrincipalInvestigator) Australian Antarctic Data Centre (publisher) Spatial: northlimit=-42.9491591; southlimit=-54.7236529; westlimit=147.3859956; eastLimit=158.9606394; projection=WGS84 Temporal: From 2018-03-09 to 2018-03-23 https://researchdata.edu.au/voyage-4-aurora-metabarcoding-markers/1675086 https://doi.org/10.26179/0byh-s246 https://data.aad.gov.au/metadata/records/AAS_4556_group_specific_eDNA_V4_2018 http://nla.gov.au/nla.party-617536 unknown Australian Antarctic Data Centre https://researchdata.edu.au/voyage-4-aurora-metabarcoding-markers/1675086 3923f0b6-1de0-4b42-a3a2-d154a8cf33cc doi:10.26179/0byh-s246 AAS_4556_group_specific_eDNA_V4_2018 https://data.aad.gov.au/metadata/records/AAS_4556_group_specific_eDNA_V4_2018 http://nla.gov.au/nla.party-617536 Australian Antarctic Data Centre biota oceans FISH EARTH SCIENCE BIOLOGICAL CLASSIFICATION ANIMALS/VERTEBRATES CRUSTACEANS ANIMALS/INVERTEBRATES ARTHROPODS PCR MISEQ ENVIRONMENTAL DNA METABARCODING BIODIVERSITY MONITORING ADS > Automated DNA Sequencer R/V AA > R/V Aurora Australis LABORATORY OCEAN > SOUTHERN OCEAN > MACQUARIE ISLAND SOUTHERN OCEAN GEOGRAPHIC REGION > POLAR dataset ftands https://doi.org/10.26179/0byh-s246 2021-02-08T23:23:34Z In March 2018, 23 environmental DNA (eDNA) samples (2 L of filtered seawater) were collected between Hobart, Tasmania and subantarctic Macquarie Island. These samples were processed using six different genetic metabarcoding markers targeting different taxonomic groups within the metazoan clade: A broad cytochrome c oxidase subunit I (COI) marker targeting all metazoans, and five different 16S markers targeting fish, cephalopods and crustaceans (one degenerate marker), fish (two markers of different lengths), cephalopods (one marker) and crustaceans (one marker). The aim of this study was to identify an ideal set of molecular markers to identify as many metazoan species as possible from small environmental samples, with a particular focus on vertebrates, crustaceans and cephalopods. The data and methods are described in the word file "V4 2018 eDNA group specific markers.docx", results are summarised in the excel file "Marker.detection.xlsx" and additional sample information is in the excel files "2018_11_07_eDNA-sample-info.xls" and "sample.map.csv". Each genetic marker used in this study has its own folder, containing the raw FASTQ sequencing data, the processed FASTA sequencing data, the bioinformatics processing pipeline, the zOTU fasta file, BLAST output, MEGAN output and curated zOTU table. For further explanations please refer to the word file "V4 2018 eDNA group specific markers.docx". Dataset aurora australis Macquarie Island Southern Ocean Research Data Australia (Australian National Data Service - ANDS) Southern Ocean ENVELOPE(147.3859956,158.9606394,-42.9491591,-54.7236529) |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
biota oceans FISH EARTH SCIENCE BIOLOGICAL CLASSIFICATION ANIMALS/VERTEBRATES CRUSTACEANS ANIMALS/INVERTEBRATES ARTHROPODS PCR MISEQ ENVIRONMENTAL DNA METABARCODING BIODIVERSITY MONITORING ADS > Automated DNA Sequencer R/V AA > R/V Aurora Australis LABORATORY OCEAN > SOUTHERN OCEAN > MACQUARIE ISLAND SOUTHERN OCEAN GEOGRAPHIC REGION > POLAR |
spellingShingle |
biota oceans FISH EARTH SCIENCE BIOLOGICAL CLASSIFICATION ANIMALS/VERTEBRATES CRUSTACEANS ANIMALS/INVERTEBRATES ARTHROPODS PCR MISEQ ENVIRONMENTAL DNA METABARCODING BIODIVERSITY MONITORING ADS > Automated DNA Sequencer R/V AA > R/V Aurora Australis LABORATORY OCEAN > SOUTHERN OCEAN > MACQUARIE ISLAND SOUTHERN OCEAN GEOGRAPHIC REGION > POLAR Voyage 4 of the Aurora Australis, 2018 environmental DNA samples analysed with group specific metabarcoding markers |
topic_facet |
biota oceans FISH EARTH SCIENCE BIOLOGICAL CLASSIFICATION ANIMALS/VERTEBRATES CRUSTACEANS ANIMALS/INVERTEBRATES ARTHROPODS PCR MISEQ ENVIRONMENTAL DNA METABARCODING BIODIVERSITY MONITORING ADS > Automated DNA Sequencer R/V AA > R/V Aurora Australis LABORATORY OCEAN > SOUTHERN OCEAN > MACQUARIE ISLAND SOUTHERN OCEAN GEOGRAPHIC REGION > POLAR |
description |
In March 2018, 23 environmental DNA (eDNA) samples (2 L of filtered seawater) were collected between Hobart, Tasmania and subantarctic Macquarie Island. These samples were processed using six different genetic metabarcoding markers targeting different taxonomic groups within the metazoan clade: A broad cytochrome c oxidase subunit I (COI) marker targeting all metazoans, and five different 16S markers targeting fish, cephalopods and crustaceans (one degenerate marker), fish (two markers of different lengths), cephalopods (one marker) and crustaceans (one marker). The aim of this study was to identify an ideal set of molecular markers to identify as many metazoan species as possible from small environmental samples, with a particular focus on vertebrates, crustaceans and cephalopods. The data and methods are described in the word file "V4 2018 eDNA group specific markers.docx", results are summarised in the excel file "Marker.detection.xlsx" and additional sample information is in the excel files "2018_11_07_eDNA-sample-info.xls" and "sample.map.csv". Each genetic marker used in this study has its own folder, containing the raw FASTQ sequencing data, the processed FASTA sequencing data, the bioinformatics processing pipeline, the zOTU fasta file, BLAST output, MEGAN output and curated zOTU table. For further explanations please refer to the word file "V4 2018 eDNA group specific markers.docx". |
author2 |
SUTER, LEONIE (hasPrincipalInvestigator) SUTER, LEONIE (processor) POLANOWSKI, ANDREA (hasPrincipalInvestigator) DEAGLE, BRUCE (hasPrincipalInvestigator) Australian Antarctic Data Centre (publisher) |
format |
Dataset |
title |
Voyage 4 of the Aurora Australis, 2018 environmental DNA samples analysed with group specific metabarcoding markers |
title_short |
Voyage 4 of the Aurora Australis, 2018 environmental DNA samples analysed with group specific metabarcoding markers |
title_full |
Voyage 4 of the Aurora Australis, 2018 environmental DNA samples analysed with group specific metabarcoding markers |
title_fullStr |
Voyage 4 of the Aurora Australis, 2018 environmental DNA samples analysed with group specific metabarcoding markers |
title_full_unstemmed |
Voyage 4 of the Aurora Australis, 2018 environmental DNA samples analysed with group specific metabarcoding markers |
title_sort |
voyage 4 of the aurora australis, 2018 environmental dna samples analysed with group specific metabarcoding markers |
publisher |
Australian Antarctic Data Centre |
url |
https://researchdata.edu.au/voyage-4-aurora-metabarcoding-markers/1675086 https://doi.org/10.26179/0byh-s246 https://data.aad.gov.au/metadata/records/AAS_4556_group_specific_eDNA_V4_2018 http://nla.gov.au/nla.party-617536 |
op_coverage |
Spatial: northlimit=-42.9491591; southlimit=-54.7236529; westlimit=147.3859956; eastLimit=158.9606394; projection=WGS84 Temporal: From 2018-03-09 to 2018-03-23 |
long_lat |
ENVELOPE(147.3859956,158.9606394,-42.9491591,-54.7236529) |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
aurora australis Macquarie Island Southern Ocean |
genre_facet |
aurora australis Macquarie Island Southern Ocean |
op_source |
Australian Antarctic Data Centre |
op_relation |
https://researchdata.edu.au/voyage-4-aurora-metabarcoding-markers/1675086 3923f0b6-1de0-4b42-a3a2-d154a8cf33cc doi:10.26179/0byh-s246 AAS_4556_group_specific_eDNA_V4_2018 https://data.aad.gov.au/metadata/records/AAS_4556_group_specific_eDNA_V4_2018 http://nla.gov.au/nla.party-617536 |
op_doi |
https://doi.org/10.26179/0byh-s246 |
_version_ |
1766364408099897344 |