BAS Adelie Penguin Diet
June 2018 Adélie penguin scats were collected from Signy Island (South Orkney Islands) during crèche (December/January) 2014/15 and 2015/16 and stored in 80% Ethanol. DNA was extracted from ~30 mg of faecal material using a Promega ‘Maxwell 16' instrument and a Maxwell® 16 Tissue DNA kit. A tot...
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Format: | Dataset |
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Australian Ocean Data Network
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Online Access: | https://researchdata.ands.org.au/bas-adelie-penguin-diet/1461893 https://data.aad.gov.au/metadata/records/AAS_4556_BAS_Adelie_Diet https://data.aad.gov.au/eds/5120/download https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=AAS_4556 https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4556_BAS_Adelie_Diet |
id |
ftands:oai:ands.org.au::1461893 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
biota oceans PENGUINS EARTH SCIENCE BIOLOGICAL CLASSIFICATION ANIMALS/VERTEBRATES BIRDS USE/FEEDING HABITATS BIOSPHERE ECOLOGICAL DYNAMICS SPECIES/POPULATION INTERACTIONS INDICATOR SPECIES COMMUNITY DYNAMICS ADELIE PENGUIN DNA DIET ANALYSIS ADS > Automated DNA Sequencer LABORATORY FIELD INVESTIGATION AMD CEOS AMD/AU OCEAN > ATLANTIC OCEAN > SOUTH ATLANTIC OCEAN > SOUTH ORKNEY ISLANDS GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA > SIGNY ISLAND SOUTHERN OCEAN |
spellingShingle |
biota oceans PENGUINS EARTH SCIENCE BIOLOGICAL CLASSIFICATION ANIMALS/VERTEBRATES BIRDS USE/FEEDING HABITATS BIOSPHERE ECOLOGICAL DYNAMICS SPECIES/POPULATION INTERACTIONS INDICATOR SPECIES COMMUNITY DYNAMICS ADELIE PENGUIN DNA DIET ANALYSIS ADS > Automated DNA Sequencer LABORATORY FIELD INVESTIGATION AMD CEOS AMD/AU OCEAN > ATLANTIC OCEAN > SOUTH ATLANTIC OCEAN > SOUTH ORKNEY ISLANDS GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA > SIGNY ISLAND SOUTHERN OCEAN BAS Adelie Penguin Diet |
topic_facet |
biota oceans PENGUINS EARTH SCIENCE BIOLOGICAL CLASSIFICATION ANIMALS/VERTEBRATES BIRDS USE/FEEDING HABITATS BIOSPHERE ECOLOGICAL DYNAMICS SPECIES/POPULATION INTERACTIONS INDICATOR SPECIES COMMUNITY DYNAMICS ADELIE PENGUIN DNA DIET ANALYSIS ADS > Automated DNA Sequencer LABORATORY FIELD INVESTIGATION AMD CEOS AMD/AU OCEAN > ATLANTIC OCEAN > SOUTH ATLANTIC OCEAN > SOUTH ORKNEY ISLANDS GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA > SIGNY ISLAND SOUTHERN OCEAN |
description |
June 2018 Adélie penguin scats were collected from Signy Island (South Orkney Islands) during crèche (December/January) 2014/15 and 2015/16 and stored in 80% Ethanol. DNA was extracted from ~30 mg of faecal material using a Promega ‘Maxwell 16' instrument and a Maxwell® 16 Tissue DNA kit. A total of 450 samples were analysed: 30 extractions per week for 2015 and 60 per week for samples collected in 2016. Three DNA markers providing different taxonomic information were amplified from penguin faecal DNA. First, ALL faecal DNA samples were characterised using a highly conserved metazoan primer set that amplifies a region of the nuclear 18S gene. In addition, a subset of faecal samples from each year were also characterised with two other primer pairs that amplify a region of the mtDNA 16S gene to allow species-level identification for most fish (16S_Fish) and krill (16S_Krill) species respectively. During amplification of markers, the products were tagged with a unique pair of index primers allowing samples to be pooled and sequenced (2x150bp) on a MiSeq high-throughput DNA sequencer. - See Adelie Pengiun Diet CCAMLR paper for all of the primer/PCR details - See BAS Adelie 18s Krill and Fish subset excel spreadsheet for sample details. - See BAS Adelie 18s ALL samples fastq for 18s fastq files - See BAS Adelie 16s Krill subset fastq for 16s krill fastq files - See BAS Adelie Fish subset fastq for 16s fish fastq files ##################################################################################### November 2018 In addition we also amplified all 450 samples with the 16S_Fish marker. - See Adelie Experiment Details 16s Fish for sample details, plate layout, first and second round PCR and miseq sheet. - See BAS Adelie Fish ALL Samples fastq for 16s fish fastq files By monitoring the diets of key predators over a number of years, we can examine the relative availability of different prey items, as well as the particular characteristics of their foraging behaviour. Penguin diet variability is a key CEMP parameter and with the reduction in lavage sampling it is important to identify and assess alternative, less invasive methods as potential supplementary approaches for studying penguin diet. Diet analysis using DNA in faecal material is one alternative method which eliminates the need to handle the birds and is non-invasive. |
author2 |
AADC (originator) AU/AADC > Australian Antarctic Data Centre, Australia (resourceProvider) |
format |
Dataset |
title |
BAS Adelie Penguin Diet |
title_short |
BAS Adelie Penguin Diet |
title_full |
BAS Adelie Penguin Diet |
title_fullStr |
BAS Adelie Penguin Diet |
title_full_unstemmed |
BAS Adelie Penguin Diet |
title_sort |
bas adelie penguin diet |
publisher |
Australian Ocean Data Network |
url |
https://researchdata.ands.org.au/bas-adelie-penguin-diet/1461893 https://data.aad.gov.au/metadata/records/AAS_4556_BAS_Adelie_Diet https://data.aad.gov.au/eds/5120/download https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=AAS_4556 https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4556_BAS_Adelie_Diet |
op_coverage |
Spatial: northlimit=-60.43554; southlimit=-60.89839; westlimit=-46.12061; eastLimit=-44.31885 Temporal: From 2014-12-29 to 2016-01-20 |
long_lat |
ENVELOPE(-45.500,-45.500,-60.583,-60.583) ENVELOPE(-45.595,-45.595,-60.708,-60.708) ENVELOPE(161.983,161.983,-78.000,-78.000) ENVELOPE(-46.12061,-44.31885,-60.43554,-60.89839) |
geographic |
Southern Ocean South Orkney Islands Signy Island Handle The |
geographic_facet |
Southern Ocean South Orkney Islands Signy Island Handle The |
genre |
Adelie penguin Antarc* Antarctica Signy Island South Atlantic Ocean South Orkney Islands Southern Ocean |
genre_facet |
Adelie penguin Antarc* Antarctica Signy Island South Atlantic Ocean South Orkney Islands Southern Ocean |
op_source |
https://data.aad.gov.au |
op_relation |
https://researchdata.ands.org.au/bas-adelie-penguin-diet/1461893 6f00b92f-13e0-409c-8f14-5ab94d852c75 https://data.aad.gov.au/metadata/records/AAS_4556_BAS_Adelie_Diet https://data.aad.gov.au/eds/5120/download https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=AAS_4556 https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4556_BAS_Adelie_Diet |
_version_ |
1766373192068235264 |
spelling |
ftands:oai:ands.org.au::1461893 2023-05-15T13:04:51+02:00 BAS Adelie Penguin Diet AADC (originator) AU/AADC > Australian Antarctic Data Centre, Australia (resourceProvider) Spatial: northlimit=-60.43554; southlimit=-60.89839; westlimit=-46.12061; eastLimit=-44.31885 Temporal: From 2014-12-29 to 2016-01-20 https://researchdata.ands.org.au/bas-adelie-penguin-diet/1461893 https://data.aad.gov.au/metadata/records/AAS_4556_BAS_Adelie_Diet https://data.aad.gov.au/eds/5120/download https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=AAS_4556 https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4556_BAS_Adelie_Diet unknown Australian Ocean Data Network https://researchdata.ands.org.au/bas-adelie-penguin-diet/1461893 6f00b92f-13e0-409c-8f14-5ab94d852c75 https://data.aad.gov.au/metadata/records/AAS_4556_BAS_Adelie_Diet https://data.aad.gov.au/eds/5120/download https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=AAS_4556 https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4556_BAS_Adelie_Diet https://data.aad.gov.au biota oceans PENGUINS EARTH SCIENCE BIOLOGICAL CLASSIFICATION ANIMALS/VERTEBRATES BIRDS USE/FEEDING HABITATS BIOSPHERE ECOLOGICAL DYNAMICS SPECIES/POPULATION INTERACTIONS INDICATOR SPECIES COMMUNITY DYNAMICS ADELIE PENGUIN DNA DIET ANALYSIS ADS > Automated DNA Sequencer LABORATORY FIELD INVESTIGATION AMD CEOS AMD/AU OCEAN > ATLANTIC OCEAN > SOUTH ATLANTIC OCEAN > SOUTH ORKNEY ISLANDS GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA > SIGNY ISLAND SOUTHERN OCEAN dataset ftands 2020-06-15T22:27:58Z June 2018 Adélie penguin scats were collected from Signy Island (South Orkney Islands) during crèche (December/January) 2014/15 and 2015/16 and stored in 80% Ethanol. DNA was extracted from ~30 mg of faecal material using a Promega ‘Maxwell 16' instrument and a Maxwell® 16 Tissue DNA kit. A total of 450 samples were analysed: 30 extractions per week for 2015 and 60 per week for samples collected in 2016. Three DNA markers providing different taxonomic information were amplified from penguin faecal DNA. First, ALL faecal DNA samples were characterised using a highly conserved metazoan primer set that amplifies a region of the nuclear 18S gene. In addition, a subset of faecal samples from each year were also characterised with two other primer pairs that amplify a region of the mtDNA 16S gene to allow species-level identification for most fish (16S_Fish) and krill (16S_Krill) species respectively. During amplification of markers, the products were tagged with a unique pair of index primers allowing samples to be pooled and sequenced (2x150bp) on a MiSeq high-throughput DNA sequencer. - See Adelie Pengiun Diet CCAMLR paper for all of the primer/PCR details - See BAS Adelie 18s Krill and Fish subset excel spreadsheet for sample details. - See BAS Adelie 18s ALL samples fastq for 18s fastq files - See BAS Adelie 16s Krill subset fastq for 16s krill fastq files - See BAS Adelie Fish subset fastq for 16s fish fastq files ##################################################################################### November 2018 In addition we also amplified all 450 samples with the 16S_Fish marker. - See Adelie Experiment Details 16s Fish for sample details, plate layout, first and second round PCR and miseq sheet. - See BAS Adelie Fish ALL Samples fastq for 16s fish fastq files By monitoring the diets of key predators over a number of years, we can examine the relative availability of different prey items, as well as the particular characteristics of their foraging behaviour. Penguin diet variability is a key CEMP parameter and with the reduction in lavage sampling it is important to identify and assess alternative, less invasive methods as potential supplementary approaches for studying penguin diet. Diet analysis using DNA in faecal material is one alternative method which eliminates the need to handle the birds and is non-invasive. Dataset Adelie penguin Antarc* Antarctica Signy Island South Atlantic Ocean South Orkney Islands Southern Ocean Research Data Australia (Australian National Data Service - ANDS) Southern Ocean South Orkney Islands ENVELOPE(-45.500,-45.500,-60.583,-60.583) Signy Island ENVELOPE(-45.595,-45.595,-60.708,-60.708) Handle The ENVELOPE(161.983,161.983,-78.000,-78.000) ENVELOPE(-46.12061,-44.31885,-60.43554,-60.89839) |