Winter diet of Gentoo penguins in South Georgia
See spreadsheets - Gentoo Experiment Details 18s Each number corresponds to each worksheet 1. Samples and Date Gentoo penguin scats were collected from Cumberland Bay, South Georgia (from the Maiviken colony). Visits were made weekly between 3 April and 19 Sep 2018 (one visit in June was missed owin...
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Australian Antarctic Data Centre
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Online Access: | https://researchdata.edu.au/winter-diet-gentoo-south-georgia/1459943 https://doi.org/10.26179/5eafa8a6bcf22 https://data.aad.gov.au/metadata/records/BAS_Gentoo_Diet http://nla.gov.au/nla.party-617536 |
id |
ftands:oai:ands.org.au::1459943 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
biota oceans FISHERIES EARTH SCIENCE AQUATIC SCIENCES SPECIES COMPETITION BIOSPHERE ECOLOGICAL DYNAMICS SPECIES/POPULATION INTERACTIONS USE/FEEDING HABITATS PENGUINS BIOLOGICAL CLASSIFICATION ANIMALS/VERTEBRATES BIRDS DNA DIET ANALYSIS GENTOO PENGUIN SSTI > Satellite-to-Satellite Tracking Instrument ADS > Automated DNA Sequencer LABORATORY FIELD INVESTIGATION OCEAN > ATLANTIC OCEAN > SOUTH ATLANTIC OCEAN > SOUTH GEORGIA ISLAND > CUMBERLAND BAY GEOGRAPHIC REGION > POLAR OCEAN HARBOUR BARFF PENINSULA SOUTHERN OCEAN |
spellingShingle |
biota oceans FISHERIES EARTH SCIENCE AQUATIC SCIENCES SPECIES COMPETITION BIOSPHERE ECOLOGICAL DYNAMICS SPECIES/POPULATION INTERACTIONS USE/FEEDING HABITATS PENGUINS BIOLOGICAL CLASSIFICATION ANIMALS/VERTEBRATES BIRDS DNA DIET ANALYSIS GENTOO PENGUIN SSTI > Satellite-to-Satellite Tracking Instrument ADS > Automated DNA Sequencer LABORATORY FIELD INVESTIGATION OCEAN > ATLANTIC OCEAN > SOUTH ATLANTIC OCEAN > SOUTH GEORGIA ISLAND > CUMBERLAND BAY GEOGRAPHIC REGION > POLAR OCEAN HARBOUR BARFF PENINSULA SOUTHERN OCEAN Winter diet of Gentoo penguins in South Georgia |
topic_facet |
biota oceans FISHERIES EARTH SCIENCE AQUATIC SCIENCES SPECIES COMPETITION BIOSPHERE ECOLOGICAL DYNAMICS SPECIES/POPULATION INTERACTIONS USE/FEEDING HABITATS PENGUINS BIOLOGICAL CLASSIFICATION ANIMALS/VERTEBRATES BIRDS DNA DIET ANALYSIS GENTOO PENGUIN SSTI > Satellite-to-Satellite Tracking Instrument ADS > Automated DNA Sequencer LABORATORY FIELD INVESTIGATION OCEAN > ATLANTIC OCEAN > SOUTH ATLANTIC OCEAN > SOUTH GEORGIA ISLAND > CUMBERLAND BAY GEOGRAPHIC REGION > POLAR OCEAN HARBOUR BARFF PENINSULA SOUTHERN OCEAN |
description |
See spreadsheets - Gentoo Experiment Details 18s Each number corresponds to each worksheet 1. Samples and Date Gentoo penguin scats were collected from Cumberland Bay, South Georgia (from the Maiviken colony). Visits were made weekly between 3 April and 19 Sep 2018 (one visit in June was missed owing to avalanche risk). During each of the 24 visits, 25 fresh scats were collected, producing a total of 600 samples. Samples were scooped into a 2 ml plastic screw-top tube containing 80% ethanol with a clean spatula and frozen at -20 degrees. DNA was extracted from ~30 mg of faecal material using the Promega Maxwell RSC Tissue DNA Kit. Each extraction contained a soft part or ~500ml of EtOH slurry. The samples were spun down, the EtOH was poured off, the sample was re-suspended in 120ul of S.T.A.R buffer and homogenised. 100ul of the supernatant was added to well number 1 and samples were eluted in 100ul of TE. 2. Plate Layout Samples were diluted 1/10 and plated out on 96 well plates. Each plate had a positive control (fish, squid, shrimp DNA mix) and a negative PCR control. 3. 1st Round PCR. All samples were analysed using a highly conserved metazoan primer set that amplifies a region of the nuclear 18S gene ( McInnes et al. 2017a). The first round PCR is to amplify the target marker and add sample-specific (7bp) multiplex-identifier (MID) tags (forward and reverse primer) and Illumina sequencing primers. See sheet for PCR conditions. 4. 2nd Round PCR The second round PCR is to add sequencing adapters and additional 8 bp MIDs. See sheet for PCR conditions. 5. Miseq MiSeq genome sequencer (Illumina), using the MISEQ V2 reagent kits (300 cycles). See sheet for sample layout, i5 and i7 adapters and first round MID tags ########################################################################################### See spreadsheet - Gentoo Experiment Details Fish and Krill The scat samples containing prey DNA sequences from the 18S analysis (n=222) were characterised with two other primer pairs allow species-level identification for fish and krill. 1. Samples The samples positive for prey DNA and their plate layout 2. and 3. 1st Round PCR Krill /Degenerate The first round PCR is to amplify the target marker and add sample-specific (6bp) multiplex-identifier (MID) tags (forward and reverse primer) and Illumina sequencing primers. See sheet for PCR conditions. 4. 2nd Round PCR The second round PCR is to add sequencing adapters and additional 10 bp MIDs. See sheet for PCR conditions. 5. Miseq MiSeq genome sequencer (Illumina), using the MISEQ V2 reagent kits (300 cycles). See sheet for sample layout, R and F adapters and first round MID tags. This work was completed as part of AAS project 4556. |
author2 |
POLANOWSKI, ANDREA (hasPrincipalInvestigator) POLANOWSKI, ANDREA (processor) DEAGLE, BRUCE (hasPrincipalInvestigator) DEAGLE, BRUCE (processor) RATCLIFFE, NORMAN (hasPrincipalInvestigator) Australian Antarctic Data Centre (publisher) |
format |
Dataset |
title |
Winter diet of Gentoo penguins in South Georgia |
title_short |
Winter diet of Gentoo penguins in South Georgia |
title_full |
Winter diet of Gentoo penguins in South Georgia |
title_fullStr |
Winter diet of Gentoo penguins in South Georgia |
title_full_unstemmed |
Winter diet of Gentoo penguins in South Georgia |
title_sort |
winter diet of gentoo penguins in south georgia |
publisher |
Australian Antarctic Data Centre |
url |
https://researchdata.edu.au/winter-diet-gentoo-south-georgia/1459943 https://doi.org/10.26179/5eafa8a6bcf22 https://data.aad.gov.au/metadata/records/BAS_Gentoo_Diet http://nla.gov.au/nla.party-617536 |
op_coverage |
Spatial: northlimit=-53.94962; southlimit=-54.93977; westlimit=-38.06763; eastLimit=-35.72754; projection=WGS84 Temporal: From 2019-04-03 to 2019-09-19 |
long_lat |
ENVELOPE(-36.750,-36.750,-54.250,-54.250) ENVELOPE(69.052,69.052,-48.781,-48.781) ENVELOPE(-36.508,-36.508,-54.239,-54.239) ENVELOPE(-36.247,-36.247,-54.334,-54.334) ENVELOPE(-36.314,-36.314,-54.313,-54.313) ENVELOPE(-38.06763,-35.72754,-53.94962,-54.93977) |
geographic |
Southern Ocean South Georgia Island Cumberland Bay Maiviken Ocean Harbour Barff Peninsula |
geographic_facet |
Southern Ocean South Georgia Island Cumberland Bay Maiviken Ocean Harbour Barff Peninsula |
genre |
Gentoo penguin South Atlantic Ocean South Georgia Island Southern Ocean |
genre_facet |
Gentoo penguin South Atlantic Ocean South Georgia Island Southern Ocean |
op_source |
Australian Antarctic Data Centre |
op_relation |
https://researchdata.edu.au/winter-diet-gentoo-south-georgia/1459943 ad7bebee-1bd9-4dcf-addd-dd7c420b0bd5 doi:10.26179/5eafa8a6bcf22 BAS_Gentoo_Diet https://data.aad.gov.au/metadata/records/BAS_Gentoo_Diet http://nla.gov.au/nla.party-617536 |
op_doi |
https://doi.org/10.26179/5eafa8a6bcf22 |
_version_ |
1766006028648841216 |
spelling |
ftands:oai:ands.org.au::1459943 2023-05-15T16:19:37+02:00 Winter diet of Gentoo penguins in South Georgia POLANOWSKI, ANDREA (hasPrincipalInvestigator) POLANOWSKI, ANDREA (processor) DEAGLE, BRUCE (hasPrincipalInvestigator) DEAGLE, BRUCE (processor) RATCLIFFE, NORMAN (hasPrincipalInvestigator) Australian Antarctic Data Centre (publisher) Spatial: northlimit=-53.94962; southlimit=-54.93977; westlimit=-38.06763; eastLimit=-35.72754; projection=WGS84 Temporal: From 2019-04-03 to 2019-09-19 https://researchdata.edu.au/winter-diet-gentoo-south-georgia/1459943 https://doi.org/10.26179/5eafa8a6bcf22 https://data.aad.gov.au/metadata/records/BAS_Gentoo_Diet http://nla.gov.au/nla.party-617536 unknown Australian Antarctic Data Centre https://researchdata.edu.au/winter-diet-gentoo-south-georgia/1459943 ad7bebee-1bd9-4dcf-addd-dd7c420b0bd5 doi:10.26179/5eafa8a6bcf22 BAS_Gentoo_Diet https://data.aad.gov.au/metadata/records/BAS_Gentoo_Diet http://nla.gov.au/nla.party-617536 Australian Antarctic Data Centre biota oceans FISHERIES EARTH SCIENCE AQUATIC SCIENCES SPECIES COMPETITION BIOSPHERE ECOLOGICAL DYNAMICS SPECIES/POPULATION INTERACTIONS USE/FEEDING HABITATS PENGUINS BIOLOGICAL CLASSIFICATION ANIMALS/VERTEBRATES BIRDS DNA DIET ANALYSIS GENTOO PENGUIN SSTI > Satellite-to-Satellite Tracking Instrument ADS > Automated DNA Sequencer LABORATORY FIELD INVESTIGATION OCEAN > ATLANTIC OCEAN > SOUTH ATLANTIC OCEAN > SOUTH GEORGIA ISLAND > CUMBERLAND BAY GEOGRAPHIC REGION > POLAR OCEAN HARBOUR BARFF PENINSULA SOUTHERN OCEAN dataset ftands https://doi.org/10.26179/5eafa8a6bcf22 2021-02-08T23:22:57Z See spreadsheets - Gentoo Experiment Details 18s Each number corresponds to each worksheet 1. Samples and Date Gentoo penguin scats were collected from Cumberland Bay, South Georgia (from the Maiviken colony). Visits were made weekly between 3 April and 19 Sep 2018 (one visit in June was missed owing to avalanche risk). During each of the 24 visits, 25 fresh scats were collected, producing a total of 600 samples. Samples were scooped into a 2 ml plastic screw-top tube containing 80% ethanol with a clean spatula and frozen at -20 degrees. DNA was extracted from ~30 mg of faecal material using the Promega Maxwell RSC Tissue DNA Kit. Each extraction contained a soft part or ~500ml of EtOH slurry. The samples were spun down, the EtOH was poured off, the sample was re-suspended in 120ul of S.T.A.R buffer and homogenised. 100ul of the supernatant was added to well number 1 and samples were eluted in 100ul of TE. 2. Plate Layout Samples were diluted 1/10 and plated out on 96 well plates. Each plate had a positive control (fish, squid, shrimp DNA mix) and a negative PCR control. 3. 1st Round PCR. All samples were analysed using a highly conserved metazoan primer set that amplifies a region of the nuclear 18S gene ( McInnes et al. 2017a). The first round PCR is to amplify the target marker and add sample-specific (7bp) multiplex-identifier (MID) tags (forward and reverse primer) and Illumina sequencing primers. See sheet for PCR conditions. 4. 2nd Round PCR The second round PCR is to add sequencing adapters and additional 8 bp MIDs. See sheet for PCR conditions. 5. Miseq MiSeq genome sequencer (Illumina), using the MISEQ V2 reagent kits (300 cycles). See sheet for sample layout, i5 and i7 adapters and first round MID tags ########################################################################################### See spreadsheet - Gentoo Experiment Details Fish and Krill The scat samples containing prey DNA sequences from the 18S analysis (n=222) were characterised with two other primer pairs allow species-level identification for fish and krill. 1. Samples The samples positive for prey DNA and their plate layout 2. and 3. 1st Round PCR Krill /Degenerate The first round PCR is to amplify the target marker and add sample-specific (6bp) multiplex-identifier (MID) tags (forward and reverse primer) and Illumina sequencing primers. See sheet for PCR conditions. 4. 2nd Round PCR The second round PCR is to add sequencing adapters and additional 10 bp MIDs. See sheet for PCR conditions. 5. Miseq MiSeq genome sequencer (Illumina), using the MISEQ V2 reagent kits (300 cycles). See sheet for sample layout, R and F adapters and first round MID tags. This work was completed as part of AAS project 4556. Dataset Gentoo penguin South Atlantic Ocean South Georgia Island Southern Ocean Research Data Australia (Australian National Data Service - ANDS) Southern Ocean South Georgia Island ENVELOPE(-36.750,-36.750,-54.250,-54.250) Cumberland Bay ENVELOPE(69.052,69.052,-48.781,-48.781) Maiviken ENVELOPE(-36.508,-36.508,-54.239,-54.239) Ocean Harbour ENVELOPE(-36.247,-36.247,-54.334,-54.334) Barff Peninsula ENVELOPE(-36.314,-36.314,-54.313,-54.313) ENVELOPE(-38.06763,-35.72754,-53.94962,-54.93977) |