K-Axis mesopelagic fish DNA-based diet analysis
Many COI samples have too few sequencing reads (counts) to reliably estimate diet based on this data. See detail in Clarke et al. (2018), Deep Sea Research II, https://doi.org/10.1016/j.dsr2.2018.09.001. High-throughput DNA-sequencing data for mesopelagic fish stomach contents sampled during the Ker...
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Australian Ocean Data Network
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Online Access: | https://researchdata.ands.org.au/k-axis-mesopelagic-diet-analysis/1408968 https://data.aad.gov.au/metadata/records/AAS_4313_KAXIS_FISH_DIET https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4313_KAXIS_FISH_DIET https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=4313, 4344 https://data.aad.gov.au/eds/4866/download |
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ftands:oai:ands.org.au::1408968 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
biota oceans FISH EARTH SCIENCE BIOLOGICAL CLASSIFICATION ANIMALS/VERTEBRATES RADIOLARIANS PROTISTS AMOEBOIDS EUPHAUSIIDS (KRILL) ANIMALS/INVERTEBRATES ARTHROPODS CRUSTACEANS COPEPODS DNA 18S RIBOSOMAL DNA COI (CYTOCHROME OXIDASE I) ILLUMINA MISEQ MIDOC ADS > Automated DNA Sequencer R/V AA > R/V Aurora Australis AMD AMD/AU CEOS GEOGRAPHIC REGION > POLAR OCEAN > SOUTHERN OCEAN > SOUTHERN KERGUELEN AXIS PRINCESS ELIZABETH TROUGH |
spellingShingle |
biota oceans FISH EARTH SCIENCE BIOLOGICAL CLASSIFICATION ANIMALS/VERTEBRATES RADIOLARIANS PROTISTS AMOEBOIDS EUPHAUSIIDS (KRILL) ANIMALS/INVERTEBRATES ARTHROPODS CRUSTACEANS COPEPODS DNA 18S RIBOSOMAL DNA COI (CYTOCHROME OXIDASE I) ILLUMINA MISEQ MIDOC ADS > Automated DNA Sequencer R/V AA > R/V Aurora Australis AMD AMD/AU CEOS GEOGRAPHIC REGION > POLAR OCEAN > SOUTHERN OCEAN > SOUTHERN KERGUELEN AXIS PRINCESS ELIZABETH TROUGH K-Axis mesopelagic fish DNA-based diet analysis |
topic_facet |
biota oceans FISH EARTH SCIENCE BIOLOGICAL CLASSIFICATION ANIMALS/VERTEBRATES RADIOLARIANS PROTISTS AMOEBOIDS EUPHAUSIIDS (KRILL) ANIMALS/INVERTEBRATES ARTHROPODS CRUSTACEANS COPEPODS DNA 18S RIBOSOMAL DNA COI (CYTOCHROME OXIDASE I) ILLUMINA MISEQ MIDOC ADS > Automated DNA Sequencer R/V AA > R/V Aurora Australis AMD AMD/AU CEOS GEOGRAPHIC REGION > POLAR OCEAN > SOUTHERN OCEAN > SOUTHERN KERGUELEN AXIS PRINCESS ELIZABETH TROUGH |
description |
Many COI samples have too few sequencing reads (counts) to reliably estimate diet based on this data. See detail in Clarke et al. (2018), Deep Sea Research II, https://doi.org/10.1016/j.dsr2.2018.09.001. High-throughput DNA-sequencing data for mesopelagic fish stomach contents sampled during the Kerguelen Axis voyage (January-Februay 2016). Mesopelagic fish form an important link between zooplankton and higher trophic levels in Southern Ocean food webs, however their diets are poorly known. Most of the dietary information available comes from morphological analysis of stomach contents and to a lesser extent fatty acid and stable isotopes. DNA sequencing could substantially improve our knowledge of mesopelagic fish diets, but has not previously been applied. We used high-throughput DNA sequencing (HTS) of the 18S ribosomal DNA and mitochondrial cytochrome oxidase I (COI) to characterise stomach contents of four myctophid and one bathylagid species collected at the southern extension of the Kerguelen Plateau (southern Kerguelen Axis), one of the most productive regions in the Indian sector of the Southern Ocean. Diets of the four myctophid species were dominated by amphipods, euphausiids and copepods, whereas radiolarians and siphonophores contributed a much greater proportion of HTS reads for Bathylagus sp. Analysis of mitochondrial COI showed that all species preyed on Thysanoessa macrura, but Euphausia superba was only detected in the stomach contents of myctophids. Size-based shifts in diet were apparent, with larger individuals of both bathylagid and myctophid species more likely to consume euphausiids, but we found little evidence for regional differences in diet composition for each species over the survey area. The presence of DNA from coelenterates and other gelatinous prey in the stomach contents of all five species suggests the importance of these taxa in the diet of Southern Ocean mesopelagics has been underestimated to date. Our study demonstrates the use of DNA-based diet assessment to determine the role of mesopelagic fish and their trophic position in the Southern Ocean and inform the development of ecosystem models. For more detail, see Clarke LJ, Trebilco R, Walters A, Polanowski AM, Deagle BE (2018). DNA-based diet analysis of mesopelagic fish from the southern Kerguelen Axis. Deep Sea Research Part II: Topical Studies in Oceanography. DOI:10.1016/j.dsr2.2018.09.001. Characterise Southern Ocean mesopelagic fish diets using DNA data. In addition, test for differences in diet between fish species and regions (north and south of Kerguelen Axis voyage). |
author2 |
AADC (originator) AU/AADC > Australian Antarctic Data Centre, Australia (resourceProvider) |
format |
Dataset |
title |
K-Axis mesopelagic fish DNA-based diet analysis |
title_short |
K-Axis mesopelagic fish DNA-based diet analysis |
title_full |
K-Axis mesopelagic fish DNA-based diet analysis |
title_fullStr |
K-Axis mesopelagic fish DNA-based diet analysis |
title_full_unstemmed |
K-Axis mesopelagic fish DNA-based diet analysis |
title_sort |
k-axis mesopelagic fish dna-based diet analysis |
publisher |
Australian Ocean Data Network |
url |
https://researchdata.ands.org.au/k-axis-mesopelagic-diet-analysis/1408968 https://data.aad.gov.au/metadata/records/AAS_4313_KAXIS_FISH_DIET https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4313_KAXIS_FISH_DIET https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=4313, 4344 https://data.aad.gov.au/eds/4866/download |
op_coverage |
Spatial: northlimit=-58.86768; southlimit=-64.492642; westlimit=80.423784; eastLimit=90.210047 Temporal: From 2016-02-03 to 2016-03-07 |
long_lat |
ENVELOPE(83.000,83.000,-64.167,-64.167) ENVELOPE(80.423784,90.210047,-58.86768,-64.492642) |
geographic |
Southern Ocean Kerguelen Indian Princess Elizabeth Trough |
geographic_facet |
Southern Ocean Kerguelen Indian Princess Elizabeth Trough |
genre |
aurora australis Euphausia superba Southern Ocean Thysanoessa macrura Copepods |
genre_facet |
aurora australis Euphausia superba Southern Ocean Thysanoessa macrura Copepods |
op_source |
https://data.aad.gov.au |
op_relation |
https://researchdata.ands.org.au/k-axis-mesopelagic-diet-analysis/1408968 900f9d69-6453-4f05-83ad-cd911585fc7b https://data.aad.gov.au/metadata/records/AAS_4313_KAXIS_FISH_DIET https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4313_KAXIS_FISH_DIET https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=4313, 4344 https://data.aad.gov.au/eds/4866/download |
_version_ |
1766364439240507392 |
spelling |
ftands:oai:ands.org.au::1408968 2023-05-15T15:33:50+02:00 K-Axis mesopelagic fish DNA-based diet analysis AADC (originator) AU/AADC > Australian Antarctic Data Centre, Australia (resourceProvider) Spatial: northlimit=-58.86768; southlimit=-64.492642; westlimit=80.423784; eastLimit=90.210047 Temporal: From 2016-02-03 to 2016-03-07 https://researchdata.ands.org.au/k-axis-mesopelagic-diet-analysis/1408968 https://data.aad.gov.au/metadata/records/AAS_4313_KAXIS_FISH_DIET https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4313_KAXIS_FISH_DIET https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=4313, 4344 https://data.aad.gov.au/eds/4866/download unknown Australian Ocean Data Network https://researchdata.ands.org.au/k-axis-mesopelagic-diet-analysis/1408968 900f9d69-6453-4f05-83ad-cd911585fc7b https://data.aad.gov.au/metadata/records/AAS_4313_KAXIS_FISH_DIET https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4313_KAXIS_FISH_DIET https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=4313, 4344 https://data.aad.gov.au/eds/4866/download https://data.aad.gov.au biota oceans FISH EARTH SCIENCE BIOLOGICAL CLASSIFICATION ANIMALS/VERTEBRATES RADIOLARIANS PROTISTS AMOEBOIDS EUPHAUSIIDS (KRILL) ANIMALS/INVERTEBRATES ARTHROPODS CRUSTACEANS COPEPODS DNA 18S RIBOSOMAL DNA COI (CYTOCHROME OXIDASE I) ILLUMINA MISEQ MIDOC ADS > Automated DNA Sequencer R/V AA > R/V Aurora Australis AMD AMD/AU CEOS GEOGRAPHIC REGION > POLAR OCEAN > SOUTHERN OCEAN > SOUTHERN KERGUELEN AXIS PRINCESS ELIZABETH TROUGH dataset ftands 2020-01-05T22:39:50Z Many COI samples have too few sequencing reads (counts) to reliably estimate diet based on this data. See detail in Clarke et al. (2018), Deep Sea Research II, https://doi.org/10.1016/j.dsr2.2018.09.001. High-throughput DNA-sequencing data for mesopelagic fish stomach contents sampled during the Kerguelen Axis voyage (January-Februay 2016). Mesopelagic fish form an important link between zooplankton and higher trophic levels in Southern Ocean food webs, however their diets are poorly known. Most of the dietary information available comes from morphological analysis of stomach contents and to a lesser extent fatty acid and stable isotopes. DNA sequencing could substantially improve our knowledge of mesopelagic fish diets, but has not previously been applied. We used high-throughput DNA sequencing (HTS) of the 18S ribosomal DNA and mitochondrial cytochrome oxidase I (COI) to characterise stomach contents of four myctophid and one bathylagid species collected at the southern extension of the Kerguelen Plateau (southern Kerguelen Axis), one of the most productive regions in the Indian sector of the Southern Ocean. Diets of the four myctophid species were dominated by amphipods, euphausiids and copepods, whereas radiolarians and siphonophores contributed a much greater proportion of HTS reads for Bathylagus sp. Analysis of mitochondrial COI showed that all species preyed on Thysanoessa macrura, but Euphausia superba was only detected in the stomach contents of myctophids. Size-based shifts in diet were apparent, with larger individuals of both bathylagid and myctophid species more likely to consume euphausiids, but we found little evidence for regional differences in diet composition for each species over the survey area. The presence of DNA from coelenterates and other gelatinous prey in the stomach contents of all five species suggests the importance of these taxa in the diet of Southern Ocean mesopelagics has been underestimated to date. Our study demonstrates the use of DNA-based diet assessment to determine the role of mesopelagic fish and their trophic position in the Southern Ocean and inform the development of ecosystem models. For more detail, see Clarke LJ, Trebilco R, Walters A, Polanowski AM, Deagle BE (2018). DNA-based diet analysis of mesopelagic fish from the southern Kerguelen Axis. Deep Sea Research Part II: Topical Studies in Oceanography. DOI:10.1016/j.dsr2.2018.09.001. Characterise Southern Ocean mesopelagic fish diets using DNA data. In addition, test for differences in diet between fish species and regions (north and south of Kerguelen Axis voyage). Dataset aurora australis Euphausia superba Southern Ocean Thysanoessa macrura Copepods Research Data Australia (Australian National Data Service - ANDS) Southern Ocean Kerguelen Indian Princess Elizabeth Trough ENVELOPE(83.000,83.000,-64.167,-64.167) ENVELOPE(80.423784,90.210047,-58.86768,-64.492642) |