Antarctic Krill Gonad mRNA Transcriptome

RNA was extracted from pooled gonad tissues and tails of five sexually mature males and females, respectively, originating from the krill aquarium at the AAD in Tasmania, Australia. For RNA extractions, RNeasy mini kits (QIAGEN) were used and total RNA (8 micrograms each) was sent to Geneworks, Sout...

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Bibliographic Details
Other Authors: DEAGLE, BRUCE (hasPrincipalInvestigator), DEAGLE, BRUCE (processor), POLANOWSKI, ANDREA (processor), Australian Antarctic Data Centre (publisher)
Format: Dataset
Language:unknown
Published: Australian Antarctic Data Centre
Subjects:
biota
oceans
EUPHAUSIIDS (KRILL)
EARTH SCIENCE
BIOLOGICAL CLASSIFICATION
ANIMALS/INVERTEBRATES
ARTHROPODS
CRUSTACEANS
ANTARCTIC KRILL
TRANSCRIPTOME
MRNA
ADS &gt
Automated DNA Sequencer
LABORATORY
GEOGRAPHIC REGION &gt
POLAR
OCEAN &gt
SOUTHERN OCEAN
CONTINENT &gt
ANTARCTICA
AUSTRALIA/NEW ZEALAND &gt
AUSTRALIA
Antarctic
Southern Ocean
New Zealand
Antarc*
Antarctic Krill
Antarctica
Online Access:https://researchdata.ands.org.au/antarctic-krill-gonad-mrna-transcriptome/1385733
https://doi.org/10.26179/5cd3c8fec9ad8
https://data.aad.gov.au/metadata/records/AAS_4015_Krill_Gonad_Transcriptome
https://data.aad.gov.au/metadata/records/AAS_4015_AAS_4015_Krill_Gonad_Transriptome
http://nla.gov.au/nla.party-617536
Description
Summary:RNA was extracted from pooled gonad tissues and tails of five sexually mature males and females, respectively, originating from the krill aquarium at the AAD in Tasmania, Australia. For RNA extractions, RNeasy mini kits (QIAGEN) were used and total RNA (8 micrograms each) was sent to Geneworks, South Australia (www.geneworks.com.au), for Illumina TruSeq 75 bp paired-end sequencing in two technical replica. Reads Yield Total Yield Krill_Male_sex_a_read1_sequence.txt 8,120,993 609,074,475 bases 1,218,148,950 bases Krill_Male_sex_a_read2_sequence.txt 8,120,993 609,074,475 bases Krill_Male_sex_b_read1_sequence.txt 10,465,586 784,918,950 bases 1,569,837,900 bases Krill_Male_sex_b_read2_sequence.txt 10,465,586 784,918,950 bases Krill_Male_tissue_a_read1_sequence.txt 7,867,804 590,085,300 bases 1,180,170,600 bases Krill_Male_tissue_a_read2_sequence.txt 7,867,804 590,085,300 bases Krill_Male_tissue_b_read1_sequence.txt 10,956,251 821,718,825 bases 1,793,118,450 bases Krill_Male_tissue_b_read2_sequence.txt 10,956,251 821,718,825 bases Krill_Female_sex_read1a_sequence.txt 29,447,654 2,208,574,050 bases 4,417,148,100 bases Krill_Female_sex_read2a_sequence.txt 29,447,654 2,208,574,050 bases Krill_Female_sex_read1b_sequence.txt 18,223,515 1,366,763,625 bases 2,733,527,250 bases Krill_Female_sex_read2b_sequence.txt 18,223,515 1,366,763,625 bases The insert size for these libraries is approx 160bp.

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