Short Tailed Shearwater DREAM gene sequencing fastq files

• Other Authors: AADC (originator), AU/AADC > Australian Antarctic Data Centre, Australia (resourceProvider)
• Format: Dataset
• Language: unknown
• Published: Australian Ocean Data Network
• Subjects: biota; oceans; ALBATROSSES/PETRELS AND ALLIES; EARTH SCIENCE; BIOLOGICAL CLASSIFICATION; ANIMALS/VERTEBRATES; BIRDS; DREAM; DNA; DNA METHYLATION; CHRONOLOGICAL AGE; SHORT-TAILED SHEARWATER; ADS > Automated DNA Sequencer; LABORATORY; AMD/AU; AMD; CEOS; GEOGRAPHIC REGION > POLAR; OCEAN > SOUTHERN OCEAN; CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA > Tasmania; Flinders Island; Southern Ocean; New Zealand; Lanes; Flinders
• Online Access: https://researchdata.ands.org.au/short-tailed-shearwater-fastq-files/1358661; https://data.aad.gov.au/metadata/records/AAS_4014_shearwater_DREAM; https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4014_shearwater_DREAM; http://data.aad.gov.au/eds/4791/download; https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=AAS_4014

This data set includes unprocessed sample .fastq files from two separate Illumina NextSeq runs, labelled as 'Run_1' and 'Run_2', respectively. Sample names: e.g. STS15059, 'STS' is the abbreviation of Short-tailed shearwater. The first two digits of the numeric refer to the year of collection e.g. '15' = 2015. Finally, the following number refers to the sequential unique ID for that year, e.g. '059' is the fifty-ninth sample for the years' collection. Leg bands are also recorded and are generally a 5-digit number and are unique to the individual bird. Longitudinal samples can be identified using these band IDs. E.g. in Run_2, an individual with the band number: 52196, was collected in 2015 as 'STS15065' and again in 2017 as 'STS17044'. Run_1: N = 35 individual samples are split across 4 lanes e.g. 'STS16020_S35_L001(/L002/L003/L004)_R1_001/fastq' and need to be merged before conversion to .fasta format and downstream analysis. Run_2: N = 36 individual samples were provided as a single merged file from the service provider, e.g. 'STS15059_S34_R1_001.fastq'. Sample_info: This excel spreadsheet has information on samples as follows: 'Band': 5-digit number on leg band. 'Sample': Sample number within run. 'UID': The unique ID for collection year e.g. STS15007. 'Age': The known-age of the animal rounded to whole year. 'Index (NebNext)': The NEB index used for NGS sample identification. 'Note': Additional information on if a sample was a between or within run replicate or longitudinal replicate. Analysis of these data will be published in: [tba: R. De Paoli-Iseppi et al. 2018. Molecular Ecology Resources]. Raw .fastq sequence files for replication of published results or other genomic analyses.