Expression of Paracrine Effectors in Human Adipose‐Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos)
Adipose-derived mesenchymal stem cells (ADSCs) are promising candidates for novel cell therapeutic applications. Hibernating brown bears sustain tissue integrity and function via unknown mechanisms, which might be plasma borne. We hypothesized that plasma from hibernating bears may increase the expr...
Published in: | Clinical and Translational Science |
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2021
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Online Access: | https://vbn.aau.dk/da/publications/d5250a72-2dc4-4304-91f5-572a484c1cf6 https://doi.org/10.1111/cts.12872 https://vbn.aau.dk/ws/files/412114768/cts.12872.pdf http://www.scopus.com/inward/record.url?scp=85091017319&partnerID=8YFLogxK |
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ftalborgunivpubl:oai:pure.atira.dk:publications/d5250a72-2dc4-4304-91f5-572a484c1cf6 2024-06-23T07:57:22+00:00 Expression of Paracrine Effectors in Human Adipose‐Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) Berg von Linde, Maria Johansson, Karin Kruse, Robert Helenius, Gisela Samano, Ninos Friberg, Örjan Frøbert, Anne Mette Fröbert, Ole 2021 application/pdf https://vbn.aau.dk/da/publications/d5250a72-2dc4-4304-91f5-572a484c1cf6 https://doi.org/10.1111/cts.12872 https://vbn.aau.dk/ws/files/412114768/cts.12872.pdf http://www.scopus.com/inward/record.url?scp=85091017319&partnerID=8YFLogxK eng eng https://vbn.aau.dk/da/publications/d5250a72-2dc4-4304-91f5-572a484c1cf6 info:eu-repo/semantics/openAccess Berg von Linde , M , Johansson , K , Kruse , R , Helenius , G , Samano , N , Friberg , Ö , Frøbert , A M & Fröbert , O 2021 , ' Expression of Paracrine Effectors in Human Adipose‐Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) ' , Clinical and translational science , vol. 14 , no. 1 , pp. 317-325 . https://doi.org/10.1111/cts.12872 article 2021 ftalborgunivpubl https://doi.org/10.1111/cts.12872 2024-06-03T15:06:44Z Adipose-derived mesenchymal stem cells (ADSCs) are promising candidates for novel cell therapeutic applications. Hibernating brown bears sustain tissue integrity and function via unknown mechanisms, which might be plasma borne. We hypothesized that plasma from hibernating bears may increase the expression of favorable factors from human ADSCs. In an experimental study, ADSCs from patients with ischemic heart disease were treated with interventional media containing plasma from hibernating and active bears, respectively, and with control medium. Extracted RNA from the ADSCs was sequenced using next generation sequencing. Statistical analyses of differentially expressed genes were performed using fold change analysis, pathway analysis, and gene ontology. As a result, we found that genes associated with inflammation, such as IGF1, PGF, IL11, and TGFA, were downregulated by > 10-fold in ADSCs treated with winter plasma compared with control. Genes important for cardiovascular development, ADM, ANGPTL4, and APOL3, were upregulated in ADSCs when treated with winter plasma compared with summer plasma. ADSCs treated with bear plasma, regardless if it was from hibernating or active bears, showed downregulation of IGF1, PGF, IL11, INHBA, IER3, and HMOX1 compared with control, suggesting reduced cell growth and differentiation. This can be summarized in the conclusion that plasma from hibernating bears suppresses inflammatory genes and activates genes associated with cardiovascular development in human ADSCs. Identifying the involved regulator(s) holds therapeutic potential. Article in Journal/Newspaper Ursus arctos Aalborg University's Research Portal Clinical and Translational Science 14 1 317 325 |
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Open Polar |
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Aalborg University's Research Portal |
op_collection_id |
ftalborgunivpubl |
language |
English |
description |
Adipose-derived mesenchymal stem cells (ADSCs) are promising candidates for novel cell therapeutic applications. Hibernating brown bears sustain tissue integrity and function via unknown mechanisms, which might be plasma borne. We hypothesized that plasma from hibernating bears may increase the expression of favorable factors from human ADSCs. In an experimental study, ADSCs from patients with ischemic heart disease were treated with interventional media containing plasma from hibernating and active bears, respectively, and with control medium. Extracted RNA from the ADSCs was sequenced using next generation sequencing. Statistical analyses of differentially expressed genes were performed using fold change analysis, pathway analysis, and gene ontology. As a result, we found that genes associated with inflammation, such as IGF1, PGF, IL11, and TGFA, were downregulated by > 10-fold in ADSCs treated with winter plasma compared with control. Genes important for cardiovascular development, ADM, ANGPTL4, and APOL3, were upregulated in ADSCs when treated with winter plasma compared with summer plasma. ADSCs treated with bear plasma, regardless if it was from hibernating or active bears, showed downregulation of IGF1, PGF, IL11, INHBA, IER3, and HMOX1 compared with control, suggesting reduced cell growth and differentiation. This can be summarized in the conclusion that plasma from hibernating bears suppresses inflammatory genes and activates genes associated with cardiovascular development in human ADSCs. Identifying the involved regulator(s) holds therapeutic potential. |
format |
Article in Journal/Newspaper |
author |
Berg von Linde, Maria Johansson, Karin Kruse, Robert Helenius, Gisela Samano, Ninos Friberg, Örjan Frøbert, Anne Mette Fröbert, Ole |
spellingShingle |
Berg von Linde, Maria Johansson, Karin Kruse, Robert Helenius, Gisela Samano, Ninos Friberg, Örjan Frøbert, Anne Mette Fröbert, Ole Expression of Paracrine Effectors in Human Adipose‐Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) |
author_facet |
Berg von Linde, Maria Johansson, Karin Kruse, Robert Helenius, Gisela Samano, Ninos Friberg, Örjan Frøbert, Anne Mette Fröbert, Ole |
author_sort |
Berg von Linde, Maria |
title |
Expression of Paracrine Effectors in Human Adipose‐Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) |
title_short |
Expression of Paracrine Effectors in Human Adipose‐Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) |
title_full |
Expression of Paracrine Effectors in Human Adipose‐Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) |
title_fullStr |
Expression of Paracrine Effectors in Human Adipose‐Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) |
title_full_unstemmed |
Expression of Paracrine Effectors in Human Adipose‐Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) |
title_sort |
expression of paracrine effectors in human adipose‐derived mesenchymal stem cells treated with plasma from brown bears (ursus arctos) |
publishDate |
2021 |
url |
https://vbn.aau.dk/da/publications/d5250a72-2dc4-4304-91f5-572a484c1cf6 https://doi.org/10.1111/cts.12872 https://vbn.aau.dk/ws/files/412114768/cts.12872.pdf http://www.scopus.com/inward/record.url?scp=85091017319&partnerID=8YFLogxK |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_source |
Berg von Linde , M , Johansson , K , Kruse , R , Helenius , G , Samano , N , Friberg , Ö , Frøbert , A M & Fröbert , O 2021 , ' Expression of Paracrine Effectors in Human Adipose‐Derived Mesenchymal Stem Cells Treated With Plasma From Brown Bears (Ursus arctos) ' , Clinical and translational science , vol. 14 , no. 1 , pp. 317-325 . https://doi.org/10.1111/cts.12872 |
op_relation |
https://vbn.aau.dk/da/publications/d5250a72-2dc4-4304-91f5-572a484c1cf6 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1111/cts.12872 |
container_title |
Clinical and Translational Science |
container_volume |
14 |
container_issue |
1 |
container_start_page |
317 |
op_container_end_page |
325 |
_version_ |
1802650967226712064 |