Temporal regulation of temperature tolerances and gene expression in an arctic insect
Terrestrial arthropods in the Arctic are exposed to highly variable temperatures that frequently reach cold and warm extremes. Yet, ecophysiological studies on arctic insects typically focus on the ability of species to tolerate low temperatures, whereas studies investigating physiological adaptatio...
Published in: | Journal of Experimental Biology |
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ftalborgunivpubl:oai:pure.atira.dk:publications/595a6771-8d91-4458-9641-4f406c562a4e 2024-06-23T07:48:31+00:00 Temporal regulation of temperature tolerances and gene expression in an arctic insect Noer, Natasja Krog Nielsen, Kåre Lehmann Sverrisdóttir, Elsa Kristensen, Torsten Nygård Bahrndorff, Simon 2023-06 https://vbn.aau.dk/da/publications/595a6771-8d91-4458-9641-4f406c562a4e https://doi.org/10.1242/jeb.245097 http://www.scopus.com/inward/record.url?scp=85164733565&partnerID=8YFLogxK eng eng https://vbn.aau.dk/da/publications/595a6771-8d91-4458-9641-4f406c562a4e info:eu-repo/semantics/closedAccess Noer , N K , Nielsen , K L , Sverrisdóttir , E , Kristensen , T N & Bahrndorff , S 2023 , ' Temporal regulation of temperature tolerances and gene expression in an arctic insect ' , Journal of Experimental Biology , vol. 226 , no. 11 , jeb245097 . https://doi.org/10.1242/jeb.245097 Arctic arthropods Climate change Heat shock proteins Phenotypic plasticity RNA sequencing Thermal tolerance /dk/atira/pure/sustainabledevelopmentgoals/life_on_land name=SDG 15 - Life on Land /dk/atira/pure/sustainabledevelopmentgoals/climate_action name=SDG 13 - Climate Action article 2023 ftalborgunivpubl https://doi.org/10.1242/jeb.245097 2024-06-10T15:25:03Z Terrestrial arthropods in the Arctic are exposed to highly variable temperatures that frequently reach cold and warm extremes. Yet, ecophysiological studies on arctic insects typically focus on the ability of species to tolerate low temperatures, whereas studies investigating physiological adaptations of species to periodically warm and variable temperatures are few. In this study, we investigated temporal changes in thermal tolerances and the transcriptome in the Greenlandic seed bug Nysius groenlandicus, collected in the field across different times and temperatures in Southern Greenland. We found that plastic changes in heat and cold tolerances occurred rapidly (within hours) and at a daily scale in the field, and that these changes are correlated with diurnal temperature variation. Using RNA sequencing, we provide molecular underpinnings of the rapid adjustments in thermal tolerance across ambient field temperatures and in the laboratory. We show that transcriptional responses are sensitive to daily temperature changes, and days characterized by high temperature variation induced markedly different expression patterns than thermally stable days. Further, genes associated with laboratory-induced heat responses, including expression of heat shock proteins and vitellogenins, were shared across laboratory and field experiments, but induced at time points associated with lower temperatures in the field. Cold stress responses were not manifested at the transcriptomic level. Article in Journal/Newspaper Arctic Arctic Climate change Greenland greenlandic Aalborg University's Research Portal Arctic Greenland Journal of Experimental Biology 226 11 |
institution |
Open Polar |
collection |
Aalborg University's Research Portal |
op_collection_id |
ftalborgunivpubl |
language |
English |
topic |
Arctic arthropods Climate change Heat shock proteins Phenotypic plasticity RNA sequencing Thermal tolerance /dk/atira/pure/sustainabledevelopmentgoals/life_on_land name=SDG 15 - Life on Land /dk/atira/pure/sustainabledevelopmentgoals/climate_action name=SDG 13 - Climate Action |
spellingShingle |
Arctic arthropods Climate change Heat shock proteins Phenotypic plasticity RNA sequencing Thermal tolerance /dk/atira/pure/sustainabledevelopmentgoals/life_on_land name=SDG 15 - Life on Land /dk/atira/pure/sustainabledevelopmentgoals/climate_action name=SDG 13 - Climate Action Noer, Natasja Krog Nielsen, Kåre Lehmann Sverrisdóttir, Elsa Kristensen, Torsten Nygård Bahrndorff, Simon Temporal regulation of temperature tolerances and gene expression in an arctic insect |
topic_facet |
Arctic arthropods Climate change Heat shock proteins Phenotypic plasticity RNA sequencing Thermal tolerance /dk/atira/pure/sustainabledevelopmentgoals/life_on_land name=SDG 15 - Life on Land /dk/atira/pure/sustainabledevelopmentgoals/climate_action name=SDG 13 - Climate Action |
description |
Terrestrial arthropods in the Arctic are exposed to highly variable temperatures that frequently reach cold and warm extremes. Yet, ecophysiological studies on arctic insects typically focus on the ability of species to tolerate low temperatures, whereas studies investigating physiological adaptations of species to periodically warm and variable temperatures are few. In this study, we investigated temporal changes in thermal tolerances and the transcriptome in the Greenlandic seed bug Nysius groenlandicus, collected in the field across different times and temperatures in Southern Greenland. We found that plastic changes in heat and cold tolerances occurred rapidly (within hours) and at a daily scale in the field, and that these changes are correlated with diurnal temperature variation. Using RNA sequencing, we provide molecular underpinnings of the rapid adjustments in thermal tolerance across ambient field temperatures and in the laboratory. We show that transcriptional responses are sensitive to daily temperature changes, and days characterized by high temperature variation induced markedly different expression patterns than thermally stable days. Further, genes associated with laboratory-induced heat responses, including expression of heat shock proteins and vitellogenins, were shared across laboratory and field experiments, but induced at time points associated with lower temperatures in the field. Cold stress responses were not manifested at the transcriptomic level. |
format |
Article in Journal/Newspaper |
author |
Noer, Natasja Krog Nielsen, Kåre Lehmann Sverrisdóttir, Elsa Kristensen, Torsten Nygård Bahrndorff, Simon |
author_facet |
Noer, Natasja Krog Nielsen, Kåre Lehmann Sverrisdóttir, Elsa Kristensen, Torsten Nygård Bahrndorff, Simon |
author_sort |
Noer, Natasja Krog |
title |
Temporal regulation of temperature tolerances and gene expression in an arctic insect |
title_short |
Temporal regulation of temperature tolerances and gene expression in an arctic insect |
title_full |
Temporal regulation of temperature tolerances and gene expression in an arctic insect |
title_fullStr |
Temporal regulation of temperature tolerances and gene expression in an arctic insect |
title_full_unstemmed |
Temporal regulation of temperature tolerances and gene expression in an arctic insect |
title_sort |
temporal regulation of temperature tolerances and gene expression in an arctic insect |
publishDate |
2023 |
url |
https://vbn.aau.dk/da/publications/595a6771-8d91-4458-9641-4f406c562a4e https://doi.org/10.1242/jeb.245097 http://www.scopus.com/inward/record.url?scp=85164733565&partnerID=8YFLogxK |
geographic |
Arctic Greenland |
geographic_facet |
Arctic Greenland |
genre |
Arctic Arctic Climate change Greenland greenlandic |
genre_facet |
Arctic Arctic Climate change Greenland greenlandic |
op_source |
Noer , N K , Nielsen , K L , Sverrisdóttir , E , Kristensen , T N & Bahrndorff , S 2023 , ' Temporal regulation of temperature tolerances and gene expression in an arctic insect ' , Journal of Experimental Biology , vol. 226 , no. 11 , jeb245097 . https://doi.org/10.1242/jeb.245097 |
op_relation |
https://vbn.aau.dk/da/publications/595a6771-8d91-4458-9641-4f406c562a4e |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1242/jeb.245097 |
container_title |
Journal of Experimental Biology |
container_volume |
226 |
container_issue |
11 |
_version_ |
1802638903345152000 |