A technique to discriminate Canis mitochondrial DNA of new world and old world origins using specific primers
Genetic markers play an important role in elucidating taxonomic uncertainties for a wide range of organisms. We present a set of specific primers to distinguish between Canis mitochondrial DNA (mtDNA) of New World (North American) and Old World (Eurasian) origin using the ATP-8 region and gel electr...
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Online Access: | https://vbn.aau.dk/da/publications/1bd6f71d-f8f8-43cb-ab69-5f83c5f6c0c9 https://doi.org/10.1002/wsb.230 http://www.scopus.com/inward/record.url?scp=84878283372&partnerID=8YFLogxK |
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ftalborgunivpubl:oai:pure.atira.dk:publications/1bd6f71d-f8f8-43cb-ab69-5f83c5f6c0c9 2024-09-15T18:01:17+00:00 A technique to discriminate Canis mitochondrial DNA of new world and old world origins using specific primers Tessier, Nathalie Stronen, Astrid V. Lapointe, François Joseph 2013-03 https://vbn.aau.dk/da/publications/1bd6f71d-f8f8-43cb-ab69-5f83c5f6c0c9 https://doi.org/10.1002/wsb.230 http://www.scopus.com/inward/record.url?scp=84878283372&partnerID=8YFLogxK eng eng https://vbn.aau.dk/da/publications/1bd6f71d-f8f8-43cb-ab69-5f83c5f6c0c9 info:eu-repo/semantics/restrictedAccess Tessier , N , Stronen , A V & Lapointe , F J 2013 , ' A technique to discriminate Canis mitochondrial DNA of new world and old world origins using specific primers ' , Wildlife Society Bulletin , vol. 37 , no. 1 , pp. 216-219 . https://doi.org/10.1002/wsb.230 ATP-8 region Canis Coyote Mitochondrial DNA New World Old World Wolf article 2013 ftalborgunivpubl https://doi.org/10.1002/wsb.230 2024-08-22T00:15:02Z Genetic markers play an important role in elucidating taxonomic uncertainties for a wide range of organisms. We present a set of specific primers to distinguish between Canis mitochondrial DNA (mtDNA) of New World (North American) and Old World (Eurasian) origin using the ATP-8 region and gel electrophoresis. We amplified mtDNA from Old World (gray wolves [Canis lupus L., 1758]) and New World canids (coyotes [C. latrans Say, 1823] and eastern wolves [C. lycaon Schreber, 1775 or C. lupus lycaon]) collected during 2003-2009 in Québec, Canada, using a multiplexed primer triplet. The results showed a standard band of 150 base pairs (bp) for New World and Old World mtDNA. In addition, Old WorldmtDNA displayed a second band of 100 bp. The range extent of wolves with New World mtDNAhas important implications for canid conservation. The new method can assist conservation managers with rapid and cost-effective screening to monitor 1) the distribution and abundance of wolves with New World and Old World mtDNA, and 2) wolf-coyote hybridization, when used in combination with morphological information and other nuclear markers. Article in Journal/Newspaper Canis lupus Aalborg University's Research Portal Wildlife Society Bulletin 37 1 216 219 |
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Open Polar |
collection |
Aalborg University's Research Portal |
op_collection_id |
ftalborgunivpubl |
language |
English |
topic |
ATP-8 region Canis Coyote Mitochondrial DNA New World Old World Wolf |
spellingShingle |
ATP-8 region Canis Coyote Mitochondrial DNA New World Old World Wolf Tessier, Nathalie Stronen, Astrid V. Lapointe, François Joseph A technique to discriminate Canis mitochondrial DNA of new world and old world origins using specific primers |
topic_facet |
ATP-8 region Canis Coyote Mitochondrial DNA New World Old World Wolf |
description |
Genetic markers play an important role in elucidating taxonomic uncertainties for a wide range of organisms. We present a set of specific primers to distinguish between Canis mitochondrial DNA (mtDNA) of New World (North American) and Old World (Eurasian) origin using the ATP-8 region and gel electrophoresis. We amplified mtDNA from Old World (gray wolves [Canis lupus L., 1758]) and New World canids (coyotes [C. latrans Say, 1823] and eastern wolves [C. lycaon Schreber, 1775 or C. lupus lycaon]) collected during 2003-2009 in Québec, Canada, using a multiplexed primer triplet. The results showed a standard band of 150 base pairs (bp) for New World and Old World mtDNA. In addition, Old WorldmtDNA displayed a second band of 100 bp. The range extent of wolves with New World mtDNAhas important implications for canid conservation. The new method can assist conservation managers with rapid and cost-effective screening to monitor 1) the distribution and abundance of wolves with New World and Old World mtDNA, and 2) wolf-coyote hybridization, when used in combination with morphological information and other nuclear markers. |
format |
Article in Journal/Newspaper |
author |
Tessier, Nathalie Stronen, Astrid V. Lapointe, François Joseph |
author_facet |
Tessier, Nathalie Stronen, Astrid V. Lapointe, François Joseph |
author_sort |
Tessier, Nathalie |
title |
A technique to discriminate Canis mitochondrial DNA of new world and old world origins using specific primers |
title_short |
A technique to discriminate Canis mitochondrial DNA of new world and old world origins using specific primers |
title_full |
A technique to discriminate Canis mitochondrial DNA of new world and old world origins using specific primers |
title_fullStr |
A technique to discriminate Canis mitochondrial DNA of new world and old world origins using specific primers |
title_full_unstemmed |
A technique to discriminate Canis mitochondrial DNA of new world and old world origins using specific primers |
title_sort |
technique to discriminate canis mitochondrial dna of new world and old world origins using specific primers |
publishDate |
2013 |
url |
https://vbn.aau.dk/da/publications/1bd6f71d-f8f8-43cb-ab69-5f83c5f6c0c9 https://doi.org/10.1002/wsb.230 http://www.scopus.com/inward/record.url?scp=84878283372&partnerID=8YFLogxK |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Tessier , N , Stronen , A V & Lapointe , F J 2013 , ' A technique to discriminate Canis mitochondrial DNA of new world and old world origins using specific primers ' , Wildlife Society Bulletin , vol. 37 , no. 1 , pp. 216-219 . https://doi.org/10.1002/wsb.230 |
op_relation |
https://vbn.aau.dk/da/publications/1bd6f71d-f8f8-43cb-ab69-5f83c5f6c0c9 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1002/wsb.230 |
container_title |
Wildlife Society Bulletin |
container_volume |
37 |
container_issue |
1 |
container_start_page |
216 |
op_container_end_page |
219 |
_version_ |
1810438449339039744 |