Perchlorate salts lower the vitrification point of Bacillus Subtilis to temperatures lower than those expected beneath the Martian south pole
International audience The discovery of lakes beneath the Martian south pole has led to questions of theirhabitability. These lakes should be characterised by incredibly low temperatures,around -70°C, and high concentrations of eutectic compounds, such as perchloratesalts, in order to facilitate the...
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ftagroparistech:oai:HAL:hal-04397603v1 2024-06-09T07:49:35+00:00 Perchlorate salts lower the vitrification point of Bacillus Subtilis to temperatures lower than those expected beneath the Martian south pole Gault, Stewart Cockell, Charles, S Fonseca, Fernanda Passot, Stéphanie University of Edinburgh (Edin.) Paris-Saclay Food and Bioproduct Engineering (SayFood) AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Madrid, Spain 2023-09-18 https://hal.science/hal-04397603 en eng HAL CCSD hal-04397603 https://hal.science/hal-04397603 European Astrobiology Network Association https://hal.science/hal-04397603 European Astrobiology Network Association, Sep 2023, Madrid, Spain [SDU]Sciences of the Universe [physics] info:eu-repo/semantics/conferenceObject Conference papers 2023 ftagroparistech 2024-05-16T11:05:43Z International audience The discovery of lakes beneath the Martian south pole has led to questions of theirhabitability. These lakes should be characterised by incredibly low temperatures,around -70°C, and high concentrations of eutectic compounds, such as perchloratesalts, in order to facilitate their liquid nature at such low temperatures (1,2). Previousresearch has shown that the low temperature limit for life is physically set by theonset of intracellular vitrification, which typically occurs around -23°C (3). Thissuggests that any potential life found within these lakes would be vitrified andpreserved as opposed to being biologically active. However, as perchlorate salts cangreatly depress the freezing point of water, the question arose as to whether theycould likewise reduce the point at which cells vitrify. Using differential scanningcalorimetry and fluorescence microscopy and spectroscopy, we found that Mg(ClO4)2lowered the vitrification point of Bacillus Subtilis cells in a concentration dependentfashion from 0.6M onwards. Additionally, we found that 2.5M Mg(ClO4)2 lowered thevitrification point of Bacillus Subtilis to -83°C, therefore suggesting that cells couldremain in an active, liquid-like state beneath the Martian south pole if they couldtolerate such high perchlorate concentrations alongside extremely low temperatures.These results also represent a new theoretical low temperature limit for life.AcknowledgmentsS. Conference Object South pole AgroParisTech: HAL (Institut des sciences et industries du vivant et de l'environnement) South Pole |
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AgroParisTech: HAL (Institut des sciences et industries du vivant et de l'environnement) |
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English |
topic |
[SDU]Sciences of the Universe [physics] |
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[SDU]Sciences of the Universe [physics] Gault, Stewart Cockell, Charles, S Fonseca, Fernanda Passot, Stéphanie Perchlorate salts lower the vitrification point of Bacillus Subtilis to temperatures lower than those expected beneath the Martian south pole |
topic_facet |
[SDU]Sciences of the Universe [physics] |
description |
International audience The discovery of lakes beneath the Martian south pole has led to questions of theirhabitability. These lakes should be characterised by incredibly low temperatures,around -70°C, and high concentrations of eutectic compounds, such as perchloratesalts, in order to facilitate their liquid nature at such low temperatures (1,2). Previousresearch has shown that the low temperature limit for life is physically set by theonset of intracellular vitrification, which typically occurs around -23°C (3). Thissuggests that any potential life found within these lakes would be vitrified andpreserved as opposed to being biologically active. However, as perchlorate salts cangreatly depress the freezing point of water, the question arose as to whether theycould likewise reduce the point at which cells vitrify. Using differential scanningcalorimetry and fluorescence microscopy and spectroscopy, we found that Mg(ClO4)2lowered the vitrification point of Bacillus Subtilis cells in a concentration dependentfashion from 0.6M onwards. Additionally, we found that 2.5M Mg(ClO4)2 lowered thevitrification point of Bacillus Subtilis to -83°C, therefore suggesting that cells couldremain in an active, liquid-like state beneath the Martian south pole if they couldtolerate such high perchlorate concentrations alongside extremely low temperatures.These results also represent a new theoretical low temperature limit for life.AcknowledgmentsS. |
author2 |
University of Edinburgh (Edin.) Paris-Saclay Food and Bioproduct Engineering (SayFood) AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
format |
Conference Object |
author |
Gault, Stewart Cockell, Charles, S Fonseca, Fernanda Passot, Stéphanie |
author_facet |
Gault, Stewart Cockell, Charles, S Fonseca, Fernanda Passot, Stéphanie |
author_sort |
Gault, Stewart |
title |
Perchlorate salts lower the vitrification point of Bacillus Subtilis to temperatures lower than those expected beneath the Martian south pole |
title_short |
Perchlorate salts lower the vitrification point of Bacillus Subtilis to temperatures lower than those expected beneath the Martian south pole |
title_full |
Perchlorate salts lower the vitrification point of Bacillus Subtilis to temperatures lower than those expected beneath the Martian south pole |
title_fullStr |
Perchlorate salts lower the vitrification point of Bacillus Subtilis to temperatures lower than those expected beneath the Martian south pole |
title_full_unstemmed |
Perchlorate salts lower the vitrification point of Bacillus Subtilis to temperatures lower than those expected beneath the Martian south pole |
title_sort |
perchlorate salts lower the vitrification point of bacillus subtilis to temperatures lower than those expected beneath the martian south pole |
publisher |
HAL CCSD |
publishDate |
2023 |
url |
https://hal.science/hal-04397603 |
op_coverage |
Madrid, Spain |
geographic |
South Pole |
geographic_facet |
South Pole |
genre |
South pole |
genre_facet |
South pole |
op_source |
European Astrobiology Network Association https://hal.science/hal-04397603 European Astrobiology Network Association, Sep 2023, Madrid, Spain |
op_relation |
hal-04397603 https://hal.science/hal-04397603 |
_version_ |
1801382289349804032 |