An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls
International audience Xylan and cellulose are abundant polysaccharides in vascular plants and essential for secondary cell wall strength. Acetate or glucuronic acid decorations are exclusively found on even-numbered residues in most of the glucuronoxylan polymer. It has been proposed that this even...
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Online Access: | https://hal.inrae.fr/hal-02629187 https://doi.org/10.1038/s41477-017-0030-8 |
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ftagroparistech:oai:HAL:hal-02629187v1 2024-06-09T07:45:46+00:00 An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls Grantham, Nicholas J. Wurman-Rodrich, Joel Terrett, Oliver M. Lyczakowski, Jan J. Stott, Katherine Iuga, Dinu Simmons, Thomas J. Durand Tardif, Mylène Brown, Steven. P. Dupree, Ray Busse-Wicher, Marta Dupree, Paul Department of Biochemistry University of Cambridge UK (CAM) University of Warwick Coventry Institut Jean-Pierre Bourgin (IJPB) Institut National de la Recherche Agronomique (INRA)-AgroParisTech Centre National de la Recherche Scientifique (CNRS) Université Paris Saclay (COmUE) Leverhulme Trust Conicyt Chile Cambridge Trusts BBSRC BB/J014540/1, PR140003 BBSRC Novozymes iCASE award BB/M015432/1 EPSRC University of Warwick through Advantage West Midlands (AWM) European Regional Development Fund (ERDF) 2017 https://hal.inrae.fr/hal-02629187 https://doi.org/10.1038/s41477-017-0030-8 en eng HAL CCSD Nature Publishing Group info:eu-repo/semantics/altIdentifier/doi/10.1038/s41477-017-0030-8 info:eu-repo/semantics/altIdentifier/pmid/28993612 hal-02629187 https://hal.inrae.fr/hal-02629187 doi:10.1038/s41477-017-0030-8 PRODINRA: 421582 PUBMED: 28993612 WOS: 000414512800011 ISSN: 2055-026X EISSN: 2055-0278 Nature Plants https://hal.inrae.fr/hal-02629187 Nature Plants, 2017, 3 (11), pp.859-865. ⟨10.1038/s41477-017-0030-8⟩ https://www.nature.com/nplants/ [SDV.BV]Life Sciences [q-bio]/Vegetal Biology info:eu-repo/semantics/article Journal articles 2017 ftagroparistech https://doi.org/10.1038/s41477-017-0030-8 2024-05-16T11:12:28Z International audience Xylan and cellulose are abundant polysaccharides in vascular plants and essential for secondary cell wall strength. Acetate or glucuronic acid decorations are exclusively found on even-numbered residues in most of the glucuronoxylan polymer. It has been proposed that this even-specific positioning of the decorations might permit docking of xylan onto the hydrophilic face of a cellulose microfibril(1-3). Consequently, xylan adopts a flattened ribbon-like twofold screw conformation when bound to cellulose in the cell wall(4). Here we show that ESKIMO1/XOAT1/TBL29, a xylan-specific O-acetyltransferase, is necessary for generation of the even pattern of acetyl esters on xylan in Arabidopsis . The reduced acetylation in the esk1 mutant deregulates the position-specific activity of the xylan glucuronosyltransferase GUX1, and so the even pattern of glucuronic acid on the xylan is lost. Solid-state NMR of intact cell walls shows that, without the even-patterned xylan decorations, xylan does not interact normally with cellulose fibrils. We conclude that the even pattern of xylan substitutions seen across vascular plants enables the interaction of xylan with hydrophilic faces of cellulose fibrils, and is essential for development of normal plant secondary cell walls. Article in Journal/Newspaper eskimo* AgroParisTech: HAL (Institut des sciences et industries du vivant et de l'environnement) Nature Plants 3 11 859 865 |
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Open Polar |
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AgroParisTech: HAL (Institut des sciences et industries du vivant et de l'environnement) |
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ftagroparistech |
language |
English |
topic |
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology |
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[SDV.BV]Life Sciences [q-bio]/Vegetal Biology Grantham, Nicholas J. Wurman-Rodrich, Joel Terrett, Oliver M. Lyczakowski, Jan J. Stott, Katherine Iuga, Dinu Simmons, Thomas J. Durand Tardif, Mylène Brown, Steven. P. Dupree, Ray Busse-Wicher, Marta Dupree, Paul An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls |
topic_facet |
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology |
description |
International audience Xylan and cellulose are abundant polysaccharides in vascular plants and essential for secondary cell wall strength. Acetate or glucuronic acid decorations are exclusively found on even-numbered residues in most of the glucuronoxylan polymer. It has been proposed that this even-specific positioning of the decorations might permit docking of xylan onto the hydrophilic face of a cellulose microfibril(1-3). Consequently, xylan adopts a flattened ribbon-like twofold screw conformation when bound to cellulose in the cell wall(4). Here we show that ESKIMO1/XOAT1/TBL29, a xylan-specific O-acetyltransferase, is necessary for generation of the even pattern of acetyl esters on xylan in Arabidopsis . The reduced acetylation in the esk1 mutant deregulates the position-specific activity of the xylan glucuronosyltransferase GUX1, and so the even pattern of glucuronic acid on the xylan is lost. Solid-state NMR of intact cell walls shows that, without the even-patterned xylan decorations, xylan does not interact normally with cellulose fibrils. We conclude that the even pattern of xylan substitutions seen across vascular plants enables the interaction of xylan with hydrophilic faces of cellulose fibrils, and is essential for development of normal plant secondary cell walls. |
author2 |
Department of Biochemistry University of Cambridge UK (CAM) University of Warwick Coventry Institut Jean-Pierre Bourgin (IJPB) Institut National de la Recherche Agronomique (INRA)-AgroParisTech Centre National de la Recherche Scientifique (CNRS) Université Paris Saclay (COmUE) Leverhulme Trust Conicyt Chile Cambridge Trusts BBSRC BB/J014540/1, PR140003 BBSRC Novozymes iCASE award BB/M015432/1 EPSRC University of Warwick through Advantage West Midlands (AWM) European Regional Development Fund (ERDF) |
format |
Article in Journal/Newspaper |
author |
Grantham, Nicholas J. Wurman-Rodrich, Joel Terrett, Oliver M. Lyczakowski, Jan J. Stott, Katherine Iuga, Dinu Simmons, Thomas J. Durand Tardif, Mylène Brown, Steven. P. Dupree, Ray Busse-Wicher, Marta Dupree, Paul |
author_facet |
Grantham, Nicholas J. Wurman-Rodrich, Joel Terrett, Oliver M. Lyczakowski, Jan J. Stott, Katherine Iuga, Dinu Simmons, Thomas J. Durand Tardif, Mylène Brown, Steven. P. Dupree, Ray Busse-Wicher, Marta Dupree, Paul |
author_sort |
Grantham, Nicholas J. |
title |
An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls |
title_short |
An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls |
title_full |
An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls |
title_fullStr |
An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls |
title_full_unstemmed |
An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls |
title_sort |
even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls |
publisher |
HAL CCSD |
publishDate |
2017 |
url |
https://hal.inrae.fr/hal-02629187 https://doi.org/10.1038/s41477-017-0030-8 |
genre |
eskimo* |
genre_facet |
eskimo* |
op_source |
ISSN: 2055-026X EISSN: 2055-0278 Nature Plants https://hal.inrae.fr/hal-02629187 Nature Plants, 2017, 3 (11), pp.859-865. ⟨10.1038/s41477-017-0030-8⟩ https://www.nature.com/nplants/ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41477-017-0030-8 info:eu-repo/semantics/altIdentifier/pmid/28993612 hal-02629187 https://hal.inrae.fr/hal-02629187 doi:10.1038/s41477-017-0030-8 PRODINRA: 421582 PUBMED: 28993612 WOS: 000414512800011 |
op_doi |
https://doi.org/10.1038/s41477-017-0030-8 |
container_title |
Nature Plants |
container_volume |
3 |
container_issue |
11 |
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859 |
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865 |
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1801375302885048320 |