Urinary glucocorticoids in harbour seal (Phoca vitulina) pups during rehabilitation
Publication history: Accepted - 8 February 2023; Published online - 10 February 2023 The glucocorticoid (GC) hormone cortisol is often measured in seals to indicate their stress levels, although other endogenous GCs are usually overlooked. We investigated concentrations of four endogenous GCs in the...
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ftafbinst:oai:afbi.dspacedirect.org:20.500.12518/535 2024-09-09T19:43:47+00:00 Urinary glucocorticoids in harbour seal (Phoca vitulina) pups during rehabilitation Wilson, Susan C. Villanueva, Stella Jones, Kayleigh A. Dmitrieva, Lilia Smyth, Wesley Animal Health and Welfare 2023-02-10 application/pdf https://hdl.handle.net/20.500.12518/535 https://doi.org/10.1016/j.ygcen.2023.114227 en eng Elsevier https://hdl.handle.net/20.500.12518/535 Wilson, S.C., Villanueva, S., Jones, K.A., Dmitrieva, L. and Smyth, W. (2023) ‘Urinary glucocorticoids in harbour seal (Phoca vitulina) pups during rehabilitation’, General and Comparative Endocrinology. Elsevier BV. Available at: https://doi.org/10.1016/j.ygcen.2023.114227. 0016-6480 https://doi.org/10.1016/j.ygcen.2023.114227 © 2023 Elsevier Inc. CC-BY-NC-ND. This manuscript is distributed under a Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits distribution and reproduction for non-commercial purposes, provided the author and source are cited. Harbor seal Cortisol Glucocorticoid Rehabilitation Stress Environment Article 2023 ftafbinst https://doi.org/20.500.12518/53510.1016/j.ygcen.2023.114227 2024-06-16T23:31:00Z Publication history: Accepted - 8 February 2023; Published online - 10 February 2023 The glucocorticoid (GC) hormone cortisol is often measured in seals to indicate their stress levels, although other endogenous GCs are usually overlooked. We investigated concentrations of four endogenous GCs in the urine of “orphan” harbour seal pups in rehabilitation. We hypothesised that the GC levels would be elevated if pups were socially isolated, without water access, and with low body mass. Ninety-six samples were collected from 32 pups at four different rehabilitation centres and were analysed by Ultra Performance Liquid Chromatography and Tandem Mass Spectrometry. Median urinary creatinine (Cr) concentrations of endogenous prednisolone (31.6 ng/mg/Cr) and prednisone (31.1 ng/mg/Cr) occurred in similar magnitude to cortisol (37.0 ng/mg/Cr), while median cortisone concentrations were higher (390 ng/mg/Cr). Prednisolone and prednisone concentrations were more strongly inversely related to pup growth rate and pup mass than cortisol and cortisone. Concentrations of all four GCs decreased with mass gain for pups with water access but did not decrease for pups without water; linear mixed models indicated the interaction between these trends was significant for cortisol and cortisone, but not for prednisolone or prednisone. These results indicate the potential value of measuring all four of these endogenous GC hormones in phocid seal pups. This study has not received any grant from any funding agency. Article in Journal/Newspaper harbor seal harbour seal Phoca vitulina AFBI Repository (Agri-Food and Biosciences Institute) General and Comparative Endocrinology 335 114227 |
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Open Polar |
collection |
AFBI Repository (Agri-Food and Biosciences Institute) |
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ftafbinst |
language |
English |
topic |
Harbor seal Cortisol Glucocorticoid Rehabilitation Stress Environment |
spellingShingle |
Harbor seal Cortisol Glucocorticoid Rehabilitation Stress Environment Wilson, Susan C. Villanueva, Stella Jones, Kayleigh A. Dmitrieva, Lilia Smyth, Wesley Urinary glucocorticoids in harbour seal (Phoca vitulina) pups during rehabilitation |
topic_facet |
Harbor seal Cortisol Glucocorticoid Rehabilitation Stress Environment |
description |
Publication history: Accepted - 8 February 2023; Published online - 10 February 2023 The glucocorticoid (GC) hormone cortisol is often measured in seals to indicate their stress levels, although other endogenous GCs are usually overlooked. We investigated concentrations of four endogenous GCs in the urine of “orphan” harbour seal pups in rehabilitation. We hypothesised that the GC levels would be elevated if pups were socially isolated, without water access, and with low body mass. Ninety-six samples were collected from 32 pups at four different rehabilitation centres and were analysed by Ultra Performance Liquid Chromatography and Tandem Mass Spectrometry. Median urinary creatinine (Cr) concentrations of endogenous prednisolone (31.6 ng/mg/Cr) and prednisone (31.1 ng/mg/Cr) occurred in similar magnitude to cortisol (37.0 ng/mg/Cr), while median cortisone concentrations were higher (390 ng/mg/Cr). Prednisolone and prednisone concentrations were more strongly inversely related to pup growth rate and pup mass than cortisol and cortisone. Concentrations of all four GCs decreased with mass gain for pups with water access but did not decrease for pups without water; linear mixed models indicated the interaction between these trends was significant for cortisol and cortisone, but not for prednisolone or prednisone. These results indicate the potential value of measuring all four of these endogenous GC hormones in phocid seal pups. This study has not received any grant from any funding agency. |
author2 |
Animal Health and Welfare |
format |
Article in Journal/Newspaper |
author |
Wilson, Susan C. Villanueva, Stella Jones, Kayleigh A. Dmitrieva, Lilia Smyth, Wesley |
author_facet |
Wilson, Susan C. Villanueva, Stella Jones, Kayleigh A. Dmitrieva, Lilia Smyth, Wesley |
author_sort |
Wilson, Susan C. |
title |
Urinary glucocorticoids in harbour seal (Phoca vitulina) pups during rehabilitation |
title_short |
Urinary glucocorticoids in harbour seal (Phoca vitulina) pups during rehabilitation |
title_full |
Urinary glucocorticoids in harbour seal (Phoca vitulina) pups during rehabilitation |
title_fullStr |
Urinary glucocorticoids in harbour seal (Phoca vitulina) pups during rehabilitation |
title_full_unstemmed |
Urinary glucocorticoids in harbour seal (Phoca vitulina) pups during rehabilitation |
title_sort |
urinary glucocorticoids in harbour seal (phoca vitulina) pups during rehabilitation |
publisher |
Elsevier |
publishDate |
2023 |
url |
https://hdl.handle.net/20.500.12518/535 https://doi.org/10.1016/j.ygcen.2023.114227 |
genre |
harbor seal harbour seal Phoca vitulina |
genre_facet |
harbor seal harbour seal Phoca vitulina |
op_relation |
https://hdl.handle.net/20.500.12518/535 Wilson, S.C., Villanueva, S., Jones, K.A., Dmitrieva, L. and Smyth, W. (2023) ‘Urinary glucocorticoids in harbour seal (Phoca vitulina) pups during rehabilitation’, General and Comparative Endocrinology. Elsevier BV. Available at: https://doi.org/10.1016/j.ygcen.2023.114227. 0016-6480 https://doi.org/10.1016/j.ygcen.2023.114227 |
op_rights |
© 2023 Elsevier Inc. CC-BY-NC-ND. This manuscript is distributed under a Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits distribution and reproduction for non-commercial purposes, provided the author and source are cited. |
op_doi |
https://doi.org/20.500.12518/53510.1016/j.ygcen.2023.114227 |
container_title |
General and Comparative Endocrinology |
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335 |
container_start_page |
114227 |
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1809913465536512000 |