Synthesis of silver and gold nanoparticles-enzyme-polymer conjugate hybrids as dual-activity catalysts for chemoenzymatic cascade reactions
Funding Information: The authors thank the support of the Spanish National Research Council (CSIC). We gratefully acknowledge financial support by the Suomen Akatemia (grant numbers 298250 & 324976, J. D.), the COST action CA15106 (CHAOS) and Finnish Foundation for Technology Promotion. We also...
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ROYAL SOC CHEMISTRY
2022
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ftaaltouniv:oai:aaltodoc.aalto.fi:123456789/114111 2024-04-28T08:00:29+00:00 Synthesis of silver and gold nanoparticles-enzyme-polymer conjugate hybrids as dual-activity catalysts for chemoenzymatic cascade reactions Naapuri, Janne M. Losada-Garcia, Noelia Deska, Jan Palomo, Jose M. Department of Chemistry and Materials Science Bioorganic chemistry CSIC Aalto-yliopisto Aalto University 2022-04-21 5701-5715 application/pdf https://aaltodoc.aalto.fi/handle/123456789/114111 https://doi.org/10.1039/d2nr00361a en eng ROYAL SOC CHEMISTRY Nanoscale Volume 14, issue 15 Naapuri, J M, Losada-Garcia, N, Deska, J & Palomo, J M 2022, ' Synthesis of silver and gold nanoparticles-enzyme-polymer conjugate hybrids as dual-activity catalysts for chemoenzymatic cascade reactions ', Nanoscale, vol. 14, no. 15, pp. 5701-5715 . https://doi.org/10.1039/d2nr00361a 2040-3364 2040-3372 PURE UUID: 6b3dd6d8-ec69-442a-ab15-d06f3338f007 PURE ITEMURL: https://research.aalto.fi/en/publications/6b3dd6d8-ec69-442a-ab15-d06f3338f007 PURE LINK: http://www.scopus.com/inward/record.url?scp=85128489005&partnerID=8YFLogxK PURE FILEURL: https://research.aalto.fi/files/82457549/CHEM_Naapuri_et_al_Synthesis_of_silver_2022_Nanoscale.pdf https://aaltodoc.aalto.fi/handle/123456789/114111 URN:NBN:fi:aalto-202205042994 doi:10.1039/d2nr00361a openAccess A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä publishedVersion 2022 ftaaltouniv https://doi.org/10.1039/d2nr00361a 2024-04-10T00:25:34Z Funding Information: The authors thank the support of the Spanish National Research Council (CSIC). We gratefully acknowledge financial support by the Suomen Akatemia (grant numbers 298250 & 324976, J. D.), the COST action CA15106 (CHAOS) and Finnish Foundation for Technology Promotion. We also thank Ramiro Martínez from Novozymes. Publisher Copyright: © 2022 The Royal Society of Chemistry Novel hybrids containing silver or gold nanoparticles have been synthesized in aqueous media and at room temperature using enzymes or tailor-made enzyme-polymer conjugates, which directly induced the formation of inorganic silver or gold species. The choice of pH, protein, or bioconjugate strongly affected the final metallic nanoparticles hybrid formation. Using Candida antarctica lipase (CALB) in a solution, nanobiohybrids containing Ag2O nanoparticles of 9 nm average diameter were obtained. The use of tailor-made bioconjugates, for example, the CALB modified with dextran-aspartic acid polymer (Dext6kDa), resulted in a nanobiohybrid containing smaller Ag(0)/Ag2O nanoparticles. In the case of nanobiohybrids based on gold, Au(0) species were found in all cases. The Au-CALB hybrid contained spherical nanoparticles with 18 nm average diameter size, with a minor range of larger ones (>100 nm) while the AuNPs-CALB-Dext6kDa hybrid was formed by much smaller nanoparticles (9 nm, minor range of 22 nm), and also nanorods of 20-30/40-50 nm length. Using Thermomyces lanuginosus lipase (TLL), apart from the nanoparticle formation, nanoflowers with a diameter range of 100-200 nm were obtained. All nanobiohybrids maintained (dual) enzymatic and metallic activities. For instance, these nanobiohybrids exhibited exquisite dual-activity for hydrolysis/cycloisomerization cascades starting from allenic acetates. By merging the transition metal reactivity with the inherent lipase catalysis, allenic acetates directly converted to the corresponding O-heterocycles in enantiopure form catalysed by AgNPs-CALB-Dext6kDa, taking advantage of a ... Article in Journal/Newspaper Antarc* Antarctica Aalto University Publication Archive (Aaltodoc) Nanoscale 14 15 5701 5715 |
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Aalto University Publication Archive (Aaltodoc) |
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ftaaltouniv |
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description |
Funding Information: The authors thank the support of the Spanish National Research Council (CSIC). We gratefully acknowledge financial support by the Suomen Akatemia (grant numbers 298250 & 324976, J. D.), the COST action CA15106 (CHAOS) and Finnish Foundation for Technology Promotion. We also thank Ramiro Martínez from Novozymes. Publisher Copyright: © 2022 The Royal Society of Chemistry Novel hybrids containing silver or gold nanoparticles have been synthesized in aqueous media and at room temperature using enzymes or tailor-made enzyme-polymer conjugates, which directly induced the formation of inorganic silver or gold species. The choice of pH, protein, or bioconjugate strongly affected the final metallic nanoparticles hybrid formation. Using Candida antarctica lipase (CALB) in a solution, nanobiohybrids containing Ag2O nanoparticles of 9 nm average diameter were obtained. The use of tailor-made bioconjugates, for example, the CALB modified with dextran-aspartic acid polymer (Dext6kDa), resulted in a nanobiohybrid containing smaller Ag(0)/Ag2O nanoparticles. In the case of nanobiohybrids based on gold, Au(0) species were found in all cases. The Au-CALB hybrid contained spherical nanoparticles with 18 nm average diameter size, with a minor range of larger ones (>100 nm) while the AuNPs-CALB-Dext6kDa hybrid was formed by much smaller nanoparticles (9 nm, minor range of 22 nm), and also nanorods of 20-30/40-50 nm length. Using Thermomyces lanuginosus lipase (TLL), apart from the nanoparticle formation, nanoflowers with a diameter range of 100-200 nm were obtained. All nanobiohybrids maintained (dual) enzymatic and metallic activities. For instance, these nanobiohybrids exhibited exquisite dual-activity for hydrolysis/cycloisomerization cascades starting from allenic acetates. By merging the transition metal reactivity with the inherent lipase catalysis, allenic acetates directly converted to the corresponding O-heterocycles in enantiopure form catalysed by AgNPs-CALB-Dext6kDa, taking advantage of a ... |
author2 |
Department of Chemistry and Materials Science Bioorganic chemistry CSIC Aalto-yliopisto Aalto University |
format |
Article in Journal/Newspaper |
author |
Naapuri, Janne M. Losada-Garcia, Noelia Deska, Jan Palomo, Jose M. |
spellingShingle |
Naapuri, Janne M. Losada-Garcia, Noelia Deska, Jan Palomo, Jose M. Synthesis of silver and gold nanoparticles-enzyme-polymer conjugate hybrids as dual-activity catalysts for chemoenzymatic cascade reactions |
author_facet |
Naapuri, Janne M. Losada-Garcia, Noelia Deska, Jan Palomo, Jose M. |
author_sort |
Naapuri, Janne M. |
title |
Synthesis of silver and gold nanoparticles-enzyme-polymer conjugate hybrids as dual-activity catalysts for chemoenzymatic cascade reactions |
title_short |
Synthesis of silver and gold nanoparticles-enzyme-polymer conjugate hybrids as dual-activity catalysts for chemoenzymatic cascade reactions |
title_full |
Synthesis of silver and gold nanoparticles-enzyme-polymer conjugate hybrids as dual-activity catalysts for chemoenzymatic cascade reactions |
title_fullStr |
Synthesis of silver and gold nanoparticles-enzyme-polymer conjugate hybrids as dual-activity catalysts for chemoenzymatic cascade reactions |
title_full_unstemmed |
Synthesis of silver and gold nanoparticles-enzyme-polymer conjugate hybrids as dual-activity catalysts for chemoenzymatic cascade reactions |
title_sort |
synthesis of silver and gold nanoparticles-enzyme-polymer conjugate hybrids as dual-activity catalysts for chemoenzymatic cascade reactions |
publisher |
ROYAL SOC CHEMISTRY |
publishDate |
2022 |
url |
https://aaltodoc.aalto.fi/handle/123456789/114111 https://doi.org/10.1039/d2nr00361a |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
Nanoscale Volume 14, issue 15 Naapuri, J M, Losada-Garcia, N, Deska, J & Palomo, J M 2022, ' Synthesis of silver and gold nanoparticles-enzyme-polymer conjugate hybrids as dual-activity catalysts for chemoenzymatic cascade reactions ', Nanoscale, vol. 14, no. 15, pp. 5701-5715 . https://doi.org/10.1039/d2nr00361a 2040-3364 2040-3372 PURE UUID: 6b3dd6d8-ec69-442a-ab15-d06f3338f007 PURE ITEMURL: https://research.aalto.fi/en/publications/6b3dd6d8-ec69-442a-ab15-d06f3338f007 PURE LINK: http://www.scopus.com/inward/record.url?scp=85128489005&partnerID=8YFLogxK PURE FILEURL: https://research.aalto.fi/files/82457549/CHEM_Naapuri_et_al_Synthesis_of_silver_2022_Nanoscale.pdf https://aaltodoc.aalto.fi/handle/123456789/114111 URN:NBN:fi:aalto-202205042994 doi:10.1039/d2nr00361a |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1039/d2nr00361a |
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