Shotgun Metaproteomics of Bering Strait surface water and Chukchi Sea bottom water.

• Main Authors: William S. Noble, Brook L. Nunn
• Format: Dataset
• Language: unknown
• Published: Biological and Chemical Oceanography Data Management Office (BCO-DMO) 2017
• Subjects: biota, oceans; Bering Strait; Chukchi Sea; Chukchi
• Online Access: https://search.dataone.org/view/sha256:b7299247618e28c93b9efb6ff2f2bfe911a1cecbce0aa3c40b4326d800289420

<p><strong>Location:&nbsp;&nbsp;</strong>Water samples were collected in August of 2013 from the Bering Strait (BSt) chlorophyll maximum layer (7 m depth, 65°43.44″ N, 168°57.42″ W) and from the more northern Chukchi Sea (CS) bottom waters (55.5 m depth, 72°47.624″ N, 16°53.89″ W) using a 24-bottle CTD (conductivity, temperature, and depth) rosette (10 L General Oceanics Niskin X). The measurement of integrated water column chlorophyll was 226.88 mg/m2 at station BSt and 2.64 mg/m2 at station CS.</p> <p>Water was collected on ship, filtered, and bacterial fractions were lysed, digested and analyzed using proteomic mass spectrometry.</p> <p>Cruise = BEST Cruise 2013</p> <p>Data are available for download at the EBI PRIDE Archive. Project number = PXD006472.</p> <pre> Homepage: <a href=\"http://www.ebi.ac.uk/pride/archive\" target=\"_blank\">http://www.ebi.ac.uk/pride/archive</a> Project URL: <a href=\"http://www.ebi.ac.uk/pride/archive/projects/PXD006472\" target=\"_blank\">http://www.ebi.ac.uk/pride/archive/projects/PXD006472</a> Data URL: <a href=\"http://www.ebi.ac.uk/pride/archive/projects/PXD006472/files\" target=\"_blank\">http://www.ebi.ac.uk/pride/archive/projects/PXD006472/files</a></pre> <p>Data are published in&nbsp;May, D.H., Timmins-Schiffman, E., Mikan, M.P., Harvey, H.R., Borenstein, E., Nunn, B.L., Noble, W.S. (2016) An alignment-free \"metapeptide\" strategy for metaproteomic characterization of microbiome samples using shotgun metagenomic sequencing. Journal of Proteome Research 15, 2697-2705. DOI:&nbsp;<a href=\"http://pubs.acs.org/doi/abs/10.1021/acs.jproteome.6b00239\" target=\"_blank\">10.1021/acs.jproteome.6b00239</a></p>