Accession numbers from acidification mesocosms and original in situ samples from coastal and offshore water station at the Pivers Island Coastal Observatory January to February 2017
<p>This dataset contains Biosample and GenBank Short Read Archive (SRA) sequence accession numbers for metatranscriptomic samples obtained from acidified, warmed and control mesocosms from ocean and coastal waters. <br /> <br /> Ocean acidification (OA) is one of...
Main Authors: | , |
---|---|
Format: | Dataset |
Language: | unknown |
Published: |
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
2019
|
Subjects: | |
Online Access: | https://search.dataone.org/view/sha256:97603688d863f3d70ad16d813ffb489b0ab35b98dac142b6480aa7f49c981a10 |
id |
dataone:sha256:97603688d863f3d70ad16d813ffb489b0ab35b98dac142b6480aa7f49c981a10 |
---|---|
record_format |
openpolar |
spelling |
dataone:sha256:97603688d863f3d70ad16d813ffb489b0ab35b98dac142b6480aa7f49c981a10 2024-06-03T18:47:06+00:00 Accession numbers from acidification mesocosms and original in situ samples from coastal and offshore water station at the Pivers Island Coastal Observatory January to February 2017 Zackary I. Johnson Kostas Konstantinidis ENVELOPE(-76.67,-76.19,34.72,34.04) BEGINDATE: 2017-01-19T00:00:00Z ENDDATE: 2017-02-12T00:00:00Z 2019-04-24T00:00:00Z https://search.dataone.org/view/sha256:97603688d863f3d70ad16d813ffb489b0ab35b98dac142b6480aa7f49c981a10 unknown Biological and Chemical Oceanography Data Management Office (BCO-DMO) oceans Dataset 2019 dataone:urn:node:BCODMO 2024-06-03T18:12:03Z <p>This dataset contains Biosample and GenBank Short Read Archive (SRA) sequence accession numbers for metatranscriptomic samples obtained from acidified, warmed and control mesocosms from ocean and coastal waters. <br /> <br /> Ocean acidification (OA) is one of the major issues caused by the rising of atmospheric CO2 with immediate effects in the oceans carbonate chemistry. The oceanic microbial communities are key players in the biochemical cycles of the marine ecosystem and are expected to respond to the ocean’s changing chemistry in a feedback loop of intertwined microbial mediated processes. While the microbial response and effect to OA is challenging to predict due to the complexity of the system, experimental manipulations under controlled conditions can help us understand the major mechanisms of microbial adaptation to a changing oceanic chemistry. In this work we established replicated mesocosms in order to evaluate the effect of decreasing ph in the microbial community composition and gene expression using metatranscriptomics. We examined the effect of both decreasing ph and increasing temperature, one of the major parameters that is expected to have synergistic effects on the microbial response to OA. We examined the effect of both decreasing ph and increasing temperature, one of the major parameters that is expected to have synergistic effects on the microbial response to OA. Additionally, we established mesocosms with either coastal or ocean waters, with the expectation to observe less gene expression responses from the coastal communities which are adapted to a constantly changing water chemistry in comparison to the more sensitive to change ocean communities. In order to account for the variation in gene expression profiles from environmental samples we established two replicates for each of the following incubations (a) control (b) acidified (-0.3 from the in situ ph) (c) warmed (+3oC from the in situ temperature) (d) warmed and acidified. Each mesocosm was maintained under stable conditions for 5 days, after which we isolated samples for 16S rRNA amplicon sequencing and metatranscriptomic sequencing. Additionally, we sequenced the metatranscriptome of the original coastal and ocean samples in order to obtain a baseline gene expression profile of the in situ communities.</p> Dataset Ocean acidification Biological and Chemical Oceanography Data Management Office (BCO-DMO) (via DataONE) ENVELOPE(-76.67,-76.19,34.72,34.04) |
institution |
Open Polar |
collection |
Biological and Chemical Oceanography Data Management Office (BCO-DMO) (via DataONE) |
op_collection_id |
dataone:urn:node:BCODMO |
language |
unknown |
topic |
oceans |
spellingShingle |
oceans Zackary I. Johnson Kostas Konstantinidis Accession numbers from acidification mesocosms and original in situ samples from coastal and offshore water station at the Pivers Island Coastal Observatory January to February 2017 |
topic_facet |
oceans |
description |
<p>This dataset contains Biosample and GenBank Short Read Archive (SRA) sequence accession numbers for metatranscriptomic samples obtained from acidified, warmed and control mesocosms from ocean and coastal waters. <br /> <br /> Ocean acidification (OA) is one of the major issues caused by the rising of atmospheric CO2 with immediate effects in the oceans carbonate chemistry. The oceanic microbial communities are key players in the biochemical cycles of the marine ecosystem and are expected to respond to the ocean’s changing chemistry in a feedback loop of intertwined microbial mediated processes. While the microbial response and effect to OA is challenging to predict due to the complexity of the system, experimental manipulations under controlled conditions can help us understand the major mechanisms of microbial adaptation to a changing oceanic chemistry. In this work we established replicated mesocosms in order to evaluate the effect of decreasing ph in the microbial community composition and gene expression using metatranscriptomics. We examined the effect of both decreasing ph and increasing temperature, one of the major parameters that is expected to have synergistic effects on the microbial response to OA. We examined the effect of both decreasing ph and increasing temperature, one of the major parameters that is expected to have synergistic effects on the microbial response to OA. Additionally, we established mesocosms with either coastal or ocean waters, with the expectation to observe less gene expression responses from the coastal communities which are adapted to a constantly changing water chemistry in comparison to the more sensitive to change ocean communities. In order to account for the variation in gene expression profiles from environmental samples we established two replicates for each of the following incubations (a) control (b) acidified (-0.3 from the in situ ph) (c) warmed (+3oC from the in situ temperature) (d) warmed and acidified. Each mesocosm was maintained under stable conditions for 5 days, after which we isolated samples for 16S rRNA amplicon sequencing and metatranscriptomic sequencing. Additionally, we sequenced the metatranscriptome of the original coastal and ocean samples in order to obtain a baseline gene expression profile of the in situ communities.</p> |
format |
Dataset |
author |
Zackary I. Johnson Kostas Konstantinidis |
author_facet |
Zackary I. Johnson Kostas Konstantinidis |
author_sort |
Zackary I. Johnson |
title |
Accession numbers from acidification mesocosms and original in situ samples from coastal and offshore water station at the Pivers Island Coastal Observatory January to February 2017 |
title_short |
Accession numbers from acidification mesocosms and original in situ samples from coastal and offshore water station at the Pivers Island Coastal Observatory January to February 2017 |
title_full |
Accession numbers from acidification mesocosms and original in situ samples from coastal and offshore water station at the Pivers Island Coastal Observatory January to February 2017 |
title_fullStr |
Accession numbers from acidification mesocosms and original in situ samples from coastal and offshore water station at the Pivers Island Coastal Observatory January to February 2017 |
title_full_unstemmed |
Accession numbers from acidification mesocosms and original in situ samples from coastal and offshore water station at the Pivers Island Coastal Observatory January to February 2017 |
title_sort |
accession numbers from acidification mesocosms and original in situ samples from coastal and offshore water station at the pivers island coastal observatory january to february 2017 |
publisher |
Biological and Chemical Oceanography Data Management Office (BCO-DMO) |
publishDate |
2019 |
url |
https://search.dataone.org/view/sha256:97603688d863f3d70ad16d813ffb489b0ab35b98dac142b6480aa7f49c981a10 |
op_coverage |
ENVELOPE(-76.67,-76.19,34.72,34.04) BEGINDATE: 2017-01-19T00:00:00Z ENDDATE: 2017-02-12T00:00:00Z |
long_lat |
ENVELOPE(-76.67,-76.19,34.72,34.04) |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
_version_ |
1800876048062087168 |