Protist numbers in subtropical North Atlantic at and below 100 m collected on the R/V Pelagia (64PE356) from Galway, Ireland to Reykjavik, Iceland in 2010 (Eukaryote Microbes NAtl project)

• Main Author: Alexander B. Bochdansky
• Format: Dataset
• Language: unknown
• Published: Biological and Chemical Oceanography Data Management Office (BCO-DMO) 2015
• Subjects: biota, oceans; Norwegian Sea; Olympus; Iceland; North Atlantic
• Online Access: https://search.dataone.org/view/sha256:7c850089f0c244728b8e423049141886fc1a1199a8d4a34f158d10863392058f

<p>Excerpt from Bochdansky et al. (submitted):</p> <p>\"The Medea-2 expedition on the RV Pelagia (Royal Netherlands Institute for Sea Research) took place from mid June to mid July 2012. Twenty-four stations were sampled from the Porcupine Plains west towards the Charlie-Gibbs Fracture Zone, and then north to the Norwegian Sea. Water from six depths (ranging from 100 - 4483 m), four below 1000 m, one at the oxygen minimum zone (OMZ), and one at 100 m, was collected from 25 L Niskin bottles into 250 mL polycarbonate bottles. Various volumes of water were filtered onto 0.2 µm pore size, 25 mm diameter polycarbonate filters depending on depth: 50 mL from 100 m, 150 mL from the OMZ, and 250 mL from the deep sea and fixed the same way as stated above.&nbsp; The filters were stored in 2 mL cryovials at -80 °C immediately after filtering and shipped to Old Dominion University on dry ice. The filters were cut into 8ths.&nbsp; For prokaryote abundance, two opposite slices were stained and mounted with Vectashield DAPIon glass slides. For eukaryotic microbe abundance, two opposite filter slices were dual-stained with FITC and Vectashield DAPI, and enumerated (Morgan-Smith et al. 2011). All prokaryote and eukaryotic microbe counts were performed on an epifluorescence microscope (Olympus BX51). For prokaryotes a minimum of 50 randomly selected fields of view, 25 on each filter slice, were counted. For eukaryotic microbes, a minimum of 100 randomly selected fields of view was counted per station, 50 from each filter slice.\"</p> <p>Cell counts of eukaryotic microbes can be found in &nbsp;the&nbsp;<a href=\"http://www.bco-dmo.org/dataset-deployment/471615\" target=\"_blank\">Protist counts for the cruise Archimedes-IV&nbsp;dataset</a><strong>.</strong> Context data for this cruise are stored on the Centralized Oceanographic Data Information System (CODIS) of the Data Management Group at the Netherlands Institute of Sea Research (<a href=\"http://www.nioz.nl/portals-en\" target=\"_blank\">www.nioz.nl/portals-en</a>). Cruise number: <strong>64PE356</strong>. (not yet available,&nbsp;2015-09-01)</p> <p><strong>References:</strong></p> <p>Morgan-Smith D., Clouse M. A., Herndl G. J., Bochdansky A. B. (2013) Diversity and distribution of microbial eukaryotes in the deep tropical and subtropical North Atlantic Ocean. Deep-Sea Res. I: 78: 58-69. doi:10.1016/j.dsr.2013.04.010</p> <p>Morgan-Smith D., Herndl G. J., van Aken H. M., Bochdansky A. B. (2011) Feature article. Abundance of eukaryotic microbes in the deep subtropical North Atlantic. Aquat. Microb. Ecol. 65:103-115. doi: 10.3354/ame01536</p> <p><strong>Related datasets:</strong>&nbsp;See Dataset Collections&nbsp;under the project page for \"<a href=\"http://www.bco-dmo.org/project/2290\" target=\"_blank\">Basin-scale distribution and activity of deep-sea protists in the North Atlantic Ocean</a>\"</p>