Lipid Transfers in the Canadian Arctic

• Main Author: Marmillot, Vincent
• Format: Dataset
• Language: unknown
• Published: 2022
• Subjects: Lipids; Zooplankton; Earth and Environmental Sciences; Arctic; Other; Baffin Bay; Nares; Northwest Passage; Baffin; Beaufort Sea; Labrador Sea; Nares strait; Northwest passage; Copepods
• Online Access: https://search.dataone.org/view/sha256:6edb9cb9c6c645545d66ee6f9cb9ce5f95b2b5d8c8e54331a487db1a467a138b

MethodologyThe sampling for this dataset took place during summer 2016 between July 29 and October 2. A total of 44 stations were sampled aboard the Canadian icebreaker CCGS Amundsen in different regions of the Canadian Arctic such as Baffin Bay, Nares Strait, Northwest passage, Beaufort Sea and Labrador Sea. Zooplankton was collected with a 1-m2 aperture, 4.5-m long, conical-square plankton net with 200-um mesh size. The samples were subdivided using a Motoda splitting box. The first halves were stored with 4% formaldehyde seawater solution for further count and identification. In the second halves, macrozooplankton were removed and subdivided by size fractioning with a 750 µm mesh in order to select the largest Calanus copepods. The fraction was then subdivided using the Motoda splitting box until reaching ca 1000 mg which was deposited on a pre-burned GF/C filter and stored in pre-burned aluminium foil at -80°C until lipid analysis. The carbon content of copepod species was estimated based on the length-mass relationships established by Forest et al. (2011). At each station, 12L of water was collected at the surface and at the subsurface chlorophyll maximum in Niskin-type bottles mounted on a rosette sampler. A set of probes was used to measure the physico-chemical parameters of seawater. The pH of seawater was measured with a spectrophotometer, nutrient data were analyzed aboard from fresh samples, an aliquot was preserved in Lugol acid for taxonomic analysis and seawater filtration(3L) was conducted for further analyses of lipid contents. Phase separation methods with chloform:methanol:water was used for lipid extractions and lipid classes and fatty acid was determined using thin-layer chromatography and gas chromatography with flame ionization detection.