Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project)
<h2>Bacteria Abundance, Biomass and Chlorophyll: Water Column Samples<em>a</em></h2> <p><strong>BG 235 - Methods used for chlorophyll <em>a</em> (chla) analysis and bacteria biomass determination</strong></p> <p><strong>Core Sam...
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Biological and Chemical Oceanography Data Management Office (BCO-DMO)
2002
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Online Access: | https://search.dataone.org/view/sha256:257a268f074467aedb9fd6ea7190462a5ab77bf282a9b8a43370e229b7bb582b |
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dataone:sha256:257a268f074467aedb9fd6ea7190462a5ab77bf282a9b8a43370e229b7bb582b 2024-06-03T18:46:56+00:00 Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project) Chris H. Fritsen ENVELOPE(-72.427,-67.049,-65.965,-69.888) BEGINDATE: 2001-04-29T00:00:00Z ENDDATE: 2001-08-20T00:00:00Z 2002-11-15T00:00:00Z https://search.dataone.org/view/sha256:257a268f074467aedb9fd6ea7190462a5ab77bf282a9b8a43370e229b7bb582b unknown Biological and Chemical Oceanography Data Management Office (BCO-DMO) biota, oceans Dataset 2002 dataone:urn:node:BCODMO 2024-06-03T18:19:46Z <h2>Bacteria Abundance, Biomass and Chlorophyll: Water Column Samples<em>a</em></h2> <p><strong>BG 235 - Methods used for chlorophyll <em>a</em> (chla) analysis and bacteria biomass determination</strong></p> <p><strong>Core Sampling techniques:</strong></p> <p>Sampling methods for recovery of chlorophyll <em>a</em> and bacteria from sea ice cores follows those described in <br /> Garrison and Buck (1986)</p> <p>Recommendations for reporting were used as outlined by: Horner, R. et al.,(1992)</p> <p><strong>Analytic Techniques:</strong></p> <p>Chla (mg m<sup>-3</sup>):</p> <ul> <li>determined fluorometrically (Turner Designs 10AU Fluorometer) following extraction in 90% acetone (Parsons <em>et al</em>., 1984)</li> <li>ice core chla corrected to account for chla in filtered sea water (FSW) added to core sections during melting</li> </ul> <p>Bacteria cell abundance (cells m<sup>-3</sup>) and biomass (mg C m<sup>-3</sup>):</p> <p><strong>LMG 0106</strong></p> <ul> <li>preserved (0.5% glutaraldehyde) samples stained with 4',6-diamidino-2-phenylindole (DAPI; 0.1 to 0.3% final concentration), filtered through 0.2 mm black, polycarbonate membrane filters, and mounted onto glass microscope slides on the ship (within 24 hours following collection)</li> <li>bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus</li> <li>bacteria biomass determined using cell abundance, cell biovolume (BV; mm<sup>3</sup>; as determined from mean length and width measurements), and an allometric conversion factor for bacterial carbon per volume specific for DAPI-stained bacteria (cellular carbon = 218 X BV<sup>0.86</sup>; Loferer-Krossbacher <em>et al.</em>, 1998).</li> <li>ice core samples corrected for FSW dilution</li> </ul> <p><strong>NBP 0104</strong></p> <ul> <li>preserved (0.5% glutaraldehyde) samples stained with SYBR Gold (0.01% final concentration), filtered through 0.2 mm Anodisc filters (Whatman), and mounted onto glass microscope slides at home institution (~1-2 months following collection)</li> <li>bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus</li> <li>bacteria biomass determined using cell abundance, cell biovolume (BV; mm<sup>3</sup>), and an allometric conversion factor for bacterial carbon per volume specific for Acridine Orange-stained bacteria (cellular carbon = 89.9 X BV<sup>0.59</sup>; Simon and Azam, 1989). Note: an AO-specific carbon per volume conversion factor was used in calculating biomass in SYBR Gold-stained samples because both AO and SYBR Gold stain bacteria cells similarly relative to DAPI (unpublished data).</li> <li>ice core samples corrected for FSW dilution</li> </ul> <p>Data from <strong>LMG0106</strong> (July-August, 2001) added in June 2002.</p> <p><em>Updated: April 21, 2006</em></p> Dataset ice core Sea ice Southern Ocean Biological and Chemical Oceanography Data Management Office (BCO-DMO) (via DataONE) Southern Ocean ENVELOPE(-72.427,-67.049,-65.965,-69.888) |
institution |
Open Polar |
collection |
Biological and Chemical Oceanography Data Management Office (BCO-DMO) (via DataONE) |
op_collection_id |
dataone:urn:node:BCODMO |
language |
unknown |
topic |
biota, oceans |
spellingShingle |
biota, oceans Chris H. Fritsen Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project) |
topic_facet |
biota, oceans |
description |
<h2>Bacteria Abundance, Biomass and Chlorophyll: Water Column Samples<em>a</em></h2> <p><strong>BG 235 - Methods used for chlorophyll <em>a</em> (chla) analysis and bacteria biomass determination</strong></p> <p><strong>Core Sampling techniques:</strong></p> <p>Sampling methods for recovery of chlorophyll <em>a</em> and bacteria from sea ice cores follows those described in <br /> Garrison and Buck (1986)</p> <p>Recommendations for reporting were used as outlined by: Horner, R. et al.,(1992)</p> <p><strong>Analytic Techniques:</strong></p> <p>Chla (mg m<sup>-3</sup>):</p> <ul> <li>determined fluorometrically (Turner Designs 10AU Fluorometer) following extraction in 90% acetone (Parsons <em>et al</em>., 1984)</li> <li>ice core chla corrected to account for chla in filtered sea water (FSW) added to core sections during melting</li> </ul> <p>Bacteria cell abundance (cells m<sup>-3</sup>) and biomass (mg C m<sup>-3</sup>):</p> <p><strong>LMG 0106</strong></p> <ul> <li>preserved (0.5% glutaraldehyde) samples stained with 4',6-diamidino-2-phenylindole (DAPI; 0.1 to 0.3% final concentration), filtered through 0.2 mm black, polycarbonate membrane filters, and mounted onto glass microscope slides on the ship (within 24 hours following collection)</li> <li>bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus</li> <li>bacteria biomass determined using cell abundance, cell biovolume (BV; mm<sup>3</sup>; as determined from mean length and width measurements), and an allometric conversion factor for bacterial carbon per volume specific for DAPI-stained bacteria (cellular carbon = 218 X BV<sup>0.86</sup>; Loferer-Krossbacher <em>et al.</em>, 1998).</li> <li>ice core samples corrected for FSW dilution</li> </ul> <p><strong>NBP 0104</strong></p> <ul> <li>preserved (0.5% glutaraldehyde) samples stained with SYBR Gold (0.01% final concentration), filtered through 0.2 mm Anodisc filters (Whatman), and mounted onto glass microscope slides at home institution (~1-2 months following collection)</li> <li>bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus</li> <li>bacteria biomass determined using cell abundance, cell biovolume (BV; mm<sup>3</sup>), and an allometric conversion factor for bacterial carbon per volume specific for Acridine Orange-stained bacteria (cellular carbon = 89.9 X BV<sup>0.59</sup>; Simon and Azam, 1989). Note: an AO-specific carbon per volume conversion factor was used in calculating biomass in SYBR Gold-stained samples because both AO and SYBR Gold stain bacteria cells similarly relative to DAPI (unpublished data).</li> <li>ice core samples corrected for FSW dilution</li> </ul> <p>Data from <strong>LMG0106</strong> (July-August, 2001) added in June 2002.</p> <p><em>Updated: April 21, 2006</em></p> |
format |
Dataset |
author |
Chris H. Fritsen |
author_facet |
Chris H. Fritsen |
author_sort |
Chris H. Fritsen |
title |
Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project) |
title_short |
Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project) |
title_full |
Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project) |
title_fullStr |
Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project) |
title_full_unstemmed |
Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project) |
title_sort |
water column bacteria data from arsv laurence m. gould cruises lmg0104 and lmg0106 in the southern ocean in 2001 (soglobec project; sea ice microbes project) |
publisher |
Biological and Chemical Oceanography Data Management Office (BCO-DMO) |
publishDate |
2002 |
url |
https://search.dataone.org/view/sha256:257a268f074467aedb9fd6ea7190462a5ab77bf282a9b8a43370e229b7bb582b |
op_coverage |
ENVELOPE(-72.427,-67.049,-65.965,-69.888) BEGINDATE: 2001-04-29T00:00:00Z ENDDATE: 2001-08-20T00:00:00Z |
long_lat |
ENVELOPE(-72.427,-67.049,-65.965,-69.888) |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
ice core Sea ice Southern Ocean |
genre_facet |
ice core Sea ice Southern Ocean |
_version_ |
1800873325977665536 |