Phospholipid Fatty Acid Profiles of Bacteria and Fungi in Poor Fen Peat Exposed to Experimentally Increased N Deposition, 2015
Development of the oil sands has led to increasing atmospheric N deposition, with values as high as 17 kg N ha-1 yr-1; regional background levels <2 kg N ha-1 yr-1. To examine responses to N deposition, over five years, we experimentally applied N (as NH4NO3) to a poor fen near Mariana Lake,...
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2020
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dataone:https://pasta.lternet.edu/package/metadata/eml/edi/474/1 2024-06-03T18:46:50+00:00 Phospholipid Fatty Acid Profiles of Bacteria and Fungi in Poor Fen Peat Exposed to Experimentally Increased N Deposition, 2015 R Kelman Wieder Dale H Vitt Melanie A Vile Jeremy A Graham Jeremy A Hartsock Jacqueline M.A. Popma Hope Fillingim Melissa House James C Quinn Kimberli D Scott Meaghan Petix Kelly J McMillen Alberta, Canada, 100 km south of Fort McMurray, Canada ENVELOPE(-112.094,-112.094,55.897,55.897) BEGINDATE: 2015-07-01T00:00:00Z ENDDATE: 2015-07-31T00:00:00Z 2020-03-24T00:00:00Z https://pasta.lternet.edu/package/metadata/eml/edi/474/1 unknown Environmental Data Initiative Villanova Peatland Biogeochemistry Group MLPF PLFA fens peatland nitrogen Dataset 2020 dataone:urn:node:EDI 2024-06-03T18:16:15Z Development of the oil sands has led to increasing atmospheric N deposition, with values as high as 17 kg N ha-1 yr-1; regional background levels <2 kg N ha-1 yr-1. To examine responses to N deposition, over five years, we experimentally applied N (as NH4NO3) to a poor fen near Mariana Lake, Alberta, at rates of 0, 5, 10, 15, 20, and 25 kg N ha-1 yr-1, plus controls (no water or N addition). In July of 2015 we measured PLFA markers in two depths in each plot. Fungal abundance increased at N addition levels above 16.6 kg N ha-1 yr-1 and total bacterial abundance also increased at N addition levels above 17.1 kg N ha-1 yr-1, such that the fungal:bacterial ratio was not significantly affected by N addition. Total microbial, gram-negative bacterial, and actinomycete abundance also showed an apparent threshold responses to N addition at 16-17 kg N ha-1 yr-1 addition levels.It may be that more sensitive/targeted techniques, such as high-throughput pyrosequencing, 16s RNA clone library analysis and rRNA-targeted fluorescence in situ hybridization (FISH) or whole genome shotgun sequencing may be required to reveal detailed fen microbial community responses to N loading. Dataset Fort McMurray Environmental Data Initiative (via DataONE) Canada Fort McMurray Mariana Lake ENVELOPE(-112.024,-112.024,55.951,55.951) ENVELOPE(-112.094,-112.094,55.897,55.897) |
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Environmental Data Initiative (via DataONE) |
op_collection_id |
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language |
unknown |
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Villanova Peatland Biogeochemistry Group MLPF PLFA fens peatland nitrogen |
spellingShingle |
Villanova Peatland Biogeochemistry Group MLPF PLFA fens peatland nitrogen R Kelman Wieder Dale H Vitt Melanie A Vile Jeremy A Graham Jeremy A Hartsock Jacqueline M.A. Popma Hope Fillingim Melissa House James C Quinn Kimberli D Scott Meaghan Petix Kelly J McMillen Phospholipid Fatty Acid Profiles of Bacteria and Fungi in Poor Fen Peat Exposed to Experimentally Increased N Deposition, 2015 |
topic_facet |
Villanova Peatland Biogeochemistry Group MLPF PLFA fens peatland nitrogen |
description |
Development of the oil sands has led to increasing atmospheric N deposition, with values as high as 17 kg N ha-1 yr-1; regional background levels <2 kg N ha-1 yr-1. To examine responses to N deposition, over five years, we experimentally applied N (as NH4NO3) to a poor fen near Mariana Lake, Alberta, at rates of 0, 5, 10, 15, 20, and 25 kg N ha-1 yr-1, plus controls (no water or N addition). In July of 2015 we measured PLFA markers in two depths in each plot. Fungal abundance increased at N addition levels above 16.6 kg N ha-1 yr-1 and total bacterial abundance also increased at N addition levels above 17.1 kg N ha-1 yr-1, such that the fungal:bacterial ratio was not significantly affected by N addition. Total microbial, gram-negative bacterial, and actinomycete abundance also showed an apparent threshold responses to N addition at 16-17 kg N ha-1 yr-1 addition levels.It may be that more sensitive/targeted techniques, such as high-throughput pyrosequencing, 16s RNA clone library analysis and rRNA-targeted fluorescence in situ hybridization (FISH) or whole genome shotgun sequencing may be required to reveal detailed fen microbial community responses to N loading. |
format |
Dataset |
author |
R Kelman Wieder Dale H Vitt Melanie A Vile Jeremy A Graham Jeremy A Hartsock Jacqueline M.A. Popma Hope Fillingim Melissa House James C Quinn Kimberli D Scott Meaghan Petix Kelly J McMillen |
author_facet |
R Kelman Wieder Dale H Vitt Melanie A Vile Jeremy A Graham Jeremy A Hartsock Jacqueline M.A. Popma Hope Fillingim Melissa House James C Quinn Kimberli D Scott Meaghan Petix Kelly J McMillen |
author_sort |
R Kelman Wieder |
title |
Phospholipid Fatty Acid Profiles of Bacteria and Fungi in Poor Fen Peat Exposed to Experimentally Increased N Deposition, 2015 |
title_short |
Phospholipid Fatty Acid Profiles of Bacteria and Fungi in Poor Fen Peat Exposed to Experimentally Increased N Deposition, 2015 |
title_full |
Phospholipid Fatty Acid Profiles of Bacteria and Fungi in Poor Fen Peat Exposed to Experimentally Increased N Deposition, 2015 |
title_fullStr |
Phospholipid Fatty Acid Profiles of Bacteria and Fungi in Poor Fen Peat Exposed to Experimentally Increased N Deposition, 2015 |
title_full_unstemmed |
Phospholipid Fatty Acid Profiles of Bacteria and Fungi in Poor Fen Peat Exposed to Experimentally Increased N Deposition, 2015 |
title_sort |
phospholipid fatty acid profiles of bacteria and fungi in poor fen peat exposed to experimentally increased n deposition, 2015 |
publisher |
Environmental Data Initiative |
publishDate |
2020 |
url |
https://pasta.lternet.edu/package/metadata/eml/edi/474/1 |
op_coverage |
Alberta, Canada, 100 km south of Fort McMurray, Canada ENVELOPE(-112.094,-112.094,55.897,55.897) BEGINDATE: 2015-07-01T00:00:00Z ENDDATE: 2015-07-31T00:00:00Z |
long_lat |
ENVELOPE(-112.024,-112.024,55.951,55.951) ENVELOPE(-112.094,-112.094,55.897,55.897) |
geographic |
Canada Fort McMurray Mariana Lake |
geographic_facet |
Canada Fort McMurray Mariana Lake |
genre |
Fort McMurray |
genre_facet |
Fort McMurray |
_version_ |
1800871752222375936 |