Ice Nucleation by Marine Psychrophiles
Abstract: The primary objective of this research is to investigate polar marine psychrophilic bacteria for their potential to nucleate ice using a combination of microbiological, molecular biological and atmospheric science approaches in the laboratory. Very little is known about how psychrophiles i...
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dataone:http://get.iedadata.org/metadata/iso/600087 2024-06-03T18:46:24+00:00 Ice Nucleation by Marine Psychrophiles Swanson, Brian BEGINDATE: 2007-10-01T00:00:00Z ENDDATE: 2010-01-31T00:00:00Z 2010-01-01T00:00:00Z http://get.iedadata.org/metadata/iso/600087 unknown IEDA: US Antarctic Program Data Center Raman Spectroscopy Antarctic Organisms and Ecosystems Microbiology Biology Sea Surface Sea Ice Southern Ocean Biosphere Cryosphere Oceans US Antarctic Program Data Center (USAP-DC) Dataset 2010 dataone:urn:node:IEDA_USAP 2024-06-03T18:11:58Z Abstract: The primary objective of this research is to investigate polar marine psychrophilic bacteria for their potential to nucleate ice using a combination of microbiological, molecular biological and atmospheric science approaches in the laboratory. Very little is known about how psychrophiles interact and cope with ice or their adaptations to conditions of extreme cold and salinity. This work will involve a series of laboratory experiments using a novel freeze-tube technique for assaying freezing spectra which will provide quantitative information on: (i) the temperature-dependent freezing rates for heterogeneously frozen droplets containing sea-ice bacteria, (ii) the proportional occurrence of ice-nucleation activity versus anti-freeze activity among sea-ice bacterial isolates and (iii) the temperature-dependent freezing rates of bacteria with ice-nucleation activity grown at a range of temperatures and salinities. The compound(s) responsible for the observed activity will be identified, which is an essential step towards the development of an in-situ bacterial ice-nucleation detection assay that can be applied in the field to Antarctic water and cloud samples. One of the goals of this work is to better understand survival and cold adaptation processes of polar marine bacteria confronted with freezing conditions in sea ice. Since sea ice strongly impacts polar, as well as the global climates, this research is of significant interest because it will also provide data for accessing the importance of bacterial ice nucleation in the formation of sea ice. These measurements of ice-nucleation rates will be the first high-resolution measurements for psychrophilic marine bacteria. Another goal is to better understand the impact of bacterial ice initiation processes in polar clouds by making high-resolution measurements of nucleation rates for cloud bacteria found over Arctic and Antarctic regions. Initial measurements indicate these bacteria nucleate ice at warmer temperatures and the effect in polar regions may be quite important, since ice can strongly impact cloud dynamics, cloud radiative properties, precipitation formation, and cloud chemistry. If these initial measurements are confirmed, the data collected here will be important for improving the understanding of polar cloud processes and models. A third goal is to better understand the molecular basis of marine bacterial ice nucleation by characterizing the ice-nucleation compound and comparing it with those of known plant-derived ice-nucleating bacteria, which are the only ice-nucleating bacteria examined in detail to date. The proposed activity will support the beginning academic career of a post-doctoral researcher and will serve as the basis for several undergraduate student laboratory projects. Results from this research will be widely published in various scientific journals and outreach venues. Dataset Antarc* Antarctic Arctic Sea ice Southern Ocean IEDA: US Antarctic Program Data Center (via DataONE) Arctic Antarctic Southern Ocean |
institution |
Open Polar |
collection |
IEDA: US Antarctic Program Data Center (via DataONE) |
op_collection_id |
dataone:urn:node:IEDA_USAP |
language |
unknown |
topic |
Raman Spectroscopy Antarctic Organisms and Ecosystems Microbiology Biology Sea Surface Sea Ice Southern Ocean Biosphere Cryosphere Oceans US Antarctic Program Data Center (USAP-DC) |
spellingShingle |
Raman Spectroscopy Antarctic Organisms and Ecosystems Microbiology Biology Sea Surface Sea Ice Southern Ocean Biosphere Cryosphere Oceans US Antarctic Program Data Center (USAP-DC) Swanson, Brian Ice Nucleation by Marine Psychrophiles |
topic_facet |
Raman Spectroscopy Antarctic Organisms and Ecosystems Microbiology Biology Sea Surface Sea Ice Southern Ocean Biosphere Cryosphere Oceans US Antarctic Program Data Center (USAP-DC) |
description |
Abstract: The primary objective of this research is to investigate polar marine psychrophilic bacteria for their potential to nucleate ice using a combination of microbiological, molecular biological and atmospheric science approaches in the laboratory. Very little is known about how psychrophiles interact and cope with ice or their adaptations to conditions of extreme cold and salinity. This work will involve a series of laboratory experiments using a novel freeze-tube technique for assaying freezing spectra which will provide quantitative information on: (i) the temperature-dependent freezing rates for heterogeneously frozen droplets containing sea-ice bacteria, (ii) the proportional occurrence of ice-nucleation activity versus anti-freeze activity among sea-ice bacterial isolates and (iii) the temperature-dependent freezing rates of bacteria with ice-nucleation activity grown at a range of temperatures and salinities. The compound(s) responsible for the observed activity will be identified, which is an essential step towards the development of an in-situ bacterial ice-nucleation detection assay that can be applied in the field to Antarctic water and cloud samples. One of the goals of this work is to better understand survival and cold adaptation processes of polar marine bacteria confronted with freezing conditions in sea ice. Since sea ice strongly impacts polar, as well as the global climates, this research is of significant interest because it will also provide data for accessing the importance of bacterial ice nucleation in the formation of sea ice. These measurements of ice-nucleation rates will be the first high-resolution measurements for psychrophilic marine bacteria. Another goal is to better understand the impact of bacterial ice initiation processes in polar clouds by making high-resolution measurements of nucleation rates for cloud bacteria found over Arctic and Antarctic regions. Initial measurements indicate these bacteria nucleate ice at warmer temperatures and the effect in polar regions may be quite important, since ice can strongly impact cloud dynamics, cloud radiative properties, precipitation formation, and cloud chemistry. If these initial measurements are confirmed, the data collected here will be important for improving the understanding of polar cloud processes and models. A third goal is to better understand the molecular basis of marine bacterial ice nucleation by characterizing the ice-nucleation compound and comparing it with those of known plant-derived ice-nucleating bacteria, which are the only ice-nucleating bacteria examined in detail to date. The proposed activity will support the beginning academic career of a post-doctoral researcher and will serve as the basis for several undergraduate student laboratory projects. Results from this research will be widely published in various scientific journals and outreach venues. |
format |
Dataset |
author |
Swanson, Brian |
author_facet |
Swanson, Brian |
author_sort |
Swanson, Brian |
title |
Ice Nucleation by Marine Psychrophiles |
title_short |
Ice Nucleation by Marine Psychrophiles |
title_full |
Ice Nucleation by Marine Psychrophiles |
title_fullStr |
Ice Nucleation by Marine Psychrophiles |
title_full_unstemmed |
Ice Nucleation by Marine Psychrophiles |
title_sort |
ice nucleation by marine psychrophiles |
publisher |
IEDA: US Antarctic Program Data Center |
publishDate |
2010 |
url |
http://get.iedadata.org/metadata/iso/600087 |
op_coverage |
BEGINDATE: 2007-10-01T00:00:00Z ENDDATE: 2010-01-31T00:00:00Z |
geographic |
Arctic Antarctic Southern Ocean |
geographic_facet |
Arctic Antarctic Southern Ocean |
genre |
Antarc* Antarctic Arctic Sea ice Southern Ocean |
genre_facet |
Antarc* Antarctic Arctic Sea ice Southern Ocean |
_version_ |
1800873185610039296 |