Bacterial survival in sea ice brines: salinity shifts and the cellular fate of compatible solutes
In exploring the temperature and salinity tolerances of bacteria in sea ice, we hypothesized a seasonal synergy between bacteria and algae, based on bacterial uptake and metabolism of compatible solutes. In this model, algae release choline into the brine pockets of new sea ice, which can then be co...
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2014
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dataone:doi:10.18739/A20863591 2024-06-03T18:46:52+00:00 Bacterial survival in sea ice brines: salinity shifts and the cellular fate of compatible solutes Jody Deming VERTICAL LOCATION > SEA SURFACE, NORTH AMERICA > GREENLAND, ENVELOPE(-51.577,-51.42,64.446,64.157) BEGINDATE: 2013-03-27T00:00:00Z ENDDATE: 2014-03-23T00:00:00Z 2014-11-10T00:00:00Z https://doi.org/10.18739/A20863591 unknown Arctic Data Center EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ORGANIC CARBON EARTH SCIENCE > CRYOSPHERE > SEA ICE > ICE TEMPERATURE EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > AMMONIA EARTH SCIENCE > OCEANS > SEA ICE > SALINITY EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA EARTH SCIENCE > BIOSPHERE > AQUATIC ECOSYSTEMS > COASTAL HABITAT IN SITU/LABORATORY INSTRUMENTS > CHEMICAL METERS/ANALYZERS > FLUORESCENCE MICROSCOPY FIELD SURVEY STATION 30 METERS TO 100 METERS DAILY TO WEEKLY biota oceans Dataset 2014 dataone:urn:node:ARCTIC https://doi.org/10.18739/A20863591 2024-06-03T18:16:24Z In exploring the temperature and salinity tolerances of bacteria in sea ice, we hypothesized a seasonal synergy between bacteria and algae, based on bacterial uptake and metabolism of compatible solutes. In this model, algae release choline into the brine pockets of new sea ice, which can then be converted to compatible solutes by bacteria as a means to resist the osmotic stress of inhabiting brines that will become colder and more saline (> 210 ppt) in winter. In spring, as the ice warms and brine salinities (and osmotic stress) decrease, bacteria will metabolize these compatible solutes as nitrogen-rich carbon sources, potentially recycling ammonia to the algae. For initial testing of this hypothesis, samples of sea ice, brines and seawater were collected from Nuuk, Greenland, in spring 2013. Sample treatments included incubation at 1°C and at salinities higher than, similar to or lower than original conditions after amendment with 14C-choline. The proportions of respired choline (implying metabolism) versus retained choline (implying conversion to compatible solute for osmotic protection) were compared between treatments. Samples exposed to lower salinities respired a greater fraction of the total 14C-choline transported into cells (51103% converted to 14CO2) compared to samples exposed to higher salinities (328% respired), validating key aspects of the proposed model. Ongoing work involves similar experiments with model organisms and plans to study more natural samples obtained from coastal Greenland at different times in the lifecycle of sea ice. Dataset Greenland Nuuk Sea ice Arctic Data Center (via DataONE) Greenland Nuuk ENVELOPE(-52.150,-52.150,68.717,68.717) ENVELOPE(-51.577,-51.42,64.446,64.157) |
institution |
Open Polar |
collection |
Arctic Data Center (via DataONE) |
op_collection_id |
dataone:urn:node:ARCTIC |
language |
unknown |
topic |
EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ORGANIC CARBON EARTH SCIENCE > CRYOSPHERE > SEA ICE > ICE TEMPERATURE EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > AMMONIA EARTH SCIENCE > OCEANS > SEA ICE > SALINITY EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA EARTH SCIENCE > BIOSPHERE > AQUATIC ECOSYSTEMS > COASTAL HABITAT IN SITU/LABORATORY INSTRUMENTS > CHEMICAL METERS/ANALYZERS > FLUORESCENCE MICROSCOPY FIELD SURVEY STATION 30 METERS TO 100 METERS DAILY TO WEEKLY biota oceans |
spellingShingle |
EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ORGANIC CARBON EARTH SCIENCE > CRYOSPHERE > SEA ICE > ICE TEMPERATURE EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > AMMONIA EARTH SCIENCE > OCEANS > SEA ICE > SALINITY EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA EARTH SCIENCE > BIOSPHERE > AQUATIC ECOSYSTEMS > COASTAL HABITAT IN SITU/LABORATORY INSTRUMENTS > CHEMICAL METERS/ANALYZERS > FLUORESCENCE MICROSCOPY FIELD SURVEY STATION 30 METERS TO 100 METERS DAILY TO WEEKLY biota oceans Jody Deming Bacterial survival in sea ice brines: salinity shifts and the cellular fate of compatible solutes |
topic_facet |
EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ORGANIC CARBON EARTH SCIENCE > CRYOSPHERE > SEA ICE > ICE TEMPERATURE EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > AMMONIA EARTH SCIENCE > OCEANS > SEA ICE > SALINITY EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA EARTH SCIENCE > BIOSPHERE > AQUATIC ECOSYSTEMS > COASTAL HABITAT IN SITU/LABORATORY INSTRUMENTS > CHEMICAL METERS/ANALYZERS > FLUORESCENCE MICROSCOPY FIELD SURVEY STATION 30 METERS TO 100 METERS DAILY TO WEEKLY biota oceans |
description |
In exploring the temperature and salinity tolerances of bacteria in sea ice, we hypothesized a seasonal synergy between bacteria and algae, based on bacterial uptake and metabolism of compatible solutes. In this model, algae release choline into the brine pockets of new sea ice, which can then be converted to compatible solutes by bacteria as a means to resist the osmotic stress of inhabiting brines that will become colder and more saline (> 210 ppt) in winter. In spring, as the ice warms and brine salinities (and osmotic stress) decrease, bacteria will metabolize these compatible solutes as nitrogen-rich carbon sources, potentially recycling ammonia to the algae. For initial testing of this hypothesis, samples of sea ice, brines and seawater were collected from Nuuk, Greenland, in spring 2013. Sample treatments included incubation at 1°C and at salinities higher than, similar to or lower than original conditions after amendment with 14C-choline. The proportions of respired choline (implying metabolism) versus retained choline (implying conversion to compatible solute for osmotic protection) were compared between treatments. Samples exposed to lower salinities respired a greater fraction of the total 14C-choline transported into cells (51103% converted to 14CO2) compared to samples exposed to higher salinities (328% respired), validating key aspects of the proposed model. Ongoing work involves similar experiments with model organisms and plans to study more natural samples obtained from coastal Greenland at different times in the lifecycle of sea ice. |
format |
Dataset |
author |
Jody Deming |
author_facet |
Jody Deming |
author_sort |
Jody Deming |
title |
Bacterial survival in sea ice brines: salinity shifts and the cellular fate of compatible solutes |
title_short |
Bacterial survival in sea ice brines: salinity shifts and the cellular fate of compatible solutes |
title_full |
Bacterial survival in sea ice brines: salinity shifts and the cellular fate of compatible solutes |
title_fullStr |
Bacterial survival in sea ice brines: salinity shifts and the cellular fate of compatible solutes |
title_full_unstemmed |
Bacterial survival in sea ice brines: salinity shifts and the cellular fate of compatible solutes |
title_sort |
bacterial survival in sea ice brines: salinity shifts and the cellular fate of compatible solutes |
publisher |
Arctic Data Center |
publishDate |
2014 |
url |
https://doi.org/10.18739/A20863591 |
op_coverage |
VERTICAL LOCATION > SEA SURFACE, NORTH AMERICA > GREENLAND, ENVELOPE(-51.577,-51.42,64.446,64.157) BEGINDATE: 2013-03-27T00:00:00Z ENDDATE: 2014-03-23T00:00:00Z |
long_lat |
ENVELOPE(-52.150,-52.150,68.717,68.717) ENVELOPE(-51.577,-51.42,64.446,64.157) |
geographic |
Greenland Nuuk |
geographic_facet |
Greenland Nuuk |
genre |
Greenland Nuuk Sea ice |
genre_facet |
Greenland Nuuk Sea ice |
op_doi |
https://doi.org/10.18739/A20863591 |
_version_ |
1800872273361502208 |