Melting temperatures of DNA fragments from 32 Gulf of Alaskan rockfish species, 2013-2015

• Main Authors: Sarah Lyon, Anthony Gharrett
• Format: Dataset
• Language: unknown
• Published: Research Workspace 2019
• Subjects: OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN; OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN > GULF OF ALASKA; Sebastes zacentrus; sharpchin rockfish; Sebastes wilsoni; pygmy rockfish; Sebastes variegatus; harlequin rockfish; Sebastes variabilis; Sebastes serranoides; olive rockfish, rocote falsa cabrilla; Sebastes ruberrimus; rasphead rockfish, yelloweye rockfish, rocote ojo amarillo; Sebastes reedi; yellowmouth rockfish; Sebastes proriger; redstripe rockfish; Sebastes polyspinis; northern rockfish; Sebastes pinniger; canary rockfish, rocote canario; Sebastes paucispinis; bocaccio, rocote bocaccio; Sebastes nigrocinctus; tiger rockfish; Sebastes nebulosus; China rockfish; Sebastes mystinus; blue rockfish, rocote azul; Sebastes melanops; black rockfish; Sebastes maliger; quillback rockfish; Sebastes helvomaculatus; rosethorn rockfish; Sebastes flavidus; yellowtail rockfish; Sebastes entomelas; widow rockfish, rocote viuda; Sebastes emphaeus; Puget Sound rockfish; Sebastes elongatus; greenstriped rockfish, rocote reina; Sebastes crameri; darkblotched rockfish; Sebastes ciliatus; dusky rockfish; Sebastes caurinus; copper rockfish, rocote cobrizo; Sebastes brevispinis; silvergray rockfish; Sebastes borealis; shortraker rockfish; Sebastes babcocki; redbanded rockfish; Sebastes auriculatus; brown rockfish, rocote moreno; Sebastes alutus; Pacific ocean perch; Sebastes; rockfishes, rockcod, rosefishes; 2015; 2014; 2013; primer set; mitochondrial DNA; MT-shift; single nucleotide polymorphism; rockfish identification; Amarillo; Dixon Entrance; Falsa; Gulf of Alaska; Moreno; Pacific; Kodiak; Alaska
• Online Access: https://search.dataone.org/view/10.24431_rw1k33f_2019122023110

Data are the average and standard deviations of melting temperatures DNA fragments produced by polymerase chain reaction (PCR). The DNA fragments were amplified from primers designed to resolve single nucleotide polymorphisms (SNPs) that distinguish one or several rockfish (Sebastes spp.) species occurring in the Gulf of Alaska. The general area in which the tested species may occur is along the continental shelf from Dixon Entrance to the western end of Kodiak Island in Alaska. Each value was derived from three to five reference individuals of each species tested. The data are tabled in an EXCEL spreadsheet. Reference samples were provided by Dr. M. Love (University of California Santa Barbra) and Drs. J. Heifetz and K. Shotwell (NOAA National Marine Fisheries Service, Auke Bay Laboratories).