Successful spermatogonial stem cells transplantation within Pleuronectiformes: first breakthrough at inter-family level in marine fish
As a promising biotechnology, fish germ cell transplantation shows potentials in conservation germplasm resource, propagation of elite species, and generation of transgenic individuals. In this study, we successfully transplanted the Japanese flounder (P. olivaceus), summer flounder (P. dentatus), a...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Other/Unknown Material |
Language: | unknown |
Published: |
Authorea, Inc.
2024
|
Subjects: | |
Online Access: | http://dx.doi.org/10.22541/au.170666192.24876538/v1 |
id |
crwinnower:10.22541/au.170666192.24876538/v1 |
---|---|
record_format |
openpolar |
spelling |
crwinnower:10.22541/au.170666192.24876538/v1 2024-06-02T08:15:29+00:00 Successful spermatogonial stem cells transplantation within Pleuronectiformes: first breakthrough at inter-family level in marine fish Zhou, Li Wang, Xueying Liu, Qinghua Yang, Jingkun Xu, Shihong Wu, Zhihao Wang, Yanfeng You, Feng Song, Zongcheng Li, Jun 2024 http://dx.doi.org/10.22541/au.170666192.24876538/v1 unknown Authorea, Inc. posted-content 2024 crwinnower https://doi.org/10.22541/au.170666192.24876538/v1 2024-05-07T14:19:29Z As a promising biotechnology, fish germ cell transplantation shows potentials in conservation germplasm resource, propagation of elite species, and generation of transgenic individuals. In this study, we successfully transplanted the Japanese flounder (P. olivaceus), summer flounder (P. dentatus), and turbot (S. maximus) spermatogonia into triploid Japanese flounder larvae, and achieved high transplantation efficiency of 100%, 75-95% and 33-50% by fluorescence tracking and molecular analysis, respectively. Eventually, donor-derived spermatozoa produced offspring by artificial insemination. We only found male and intersex chimeras in inter-family transplantations, while male and female chimeras in both intra-species and intra-genus transplantations. Moreover, the intersex chimeras could mature and produce turbot functional spermatozoa. We firstly realized inter-family transplantation in marine fish species. These results demonstrated successful spermatogonial stem cells transplantation within Pleuronectiformes, suggesting the germ cells migration, incorporation and maturation within order were conserved across a wide range of teleost species. Other/Unknown Material Turbot The Winnower |
institution |
Open Polar |
collection |
The Winnower |
op_collection_id |
crwinnower |
language |
unknown |
description |
As a promising biotechnology, fish germ cell transplantation shows potentials in conservation germplasm resource, propagation of elite species, and generation of transgenic individuals. In this study, we successfully transplanted the Japanese flounder (P. olivaceus), summer flounder (P. dentatus), and turbot (S. maximus) spermatogonia into triploid Japanese flounder larvae, and achieved high transplantation efficiency of 100%, 75-95% and 33-50% by fluorescence tracking and molecular analysis, respectively. Eventually, donor-derived spermatozoa produced offspring by artificial insemination. We only found male and intersex chimeras in inter-family transplantations, while male and female chimeras in both intra-species and intra-genus transplantations. Moreover, the intersex chimeras could mature and produce turbot functional spermatozoa. We firstly realized inter-family transplantation in marine fish species. These results demonstrated successful spermatogonial stem cells transplantation within Pleuronectiformes, suggesting the germ cells migration, incorporation and maturation within order were conserved across a wide range of teleost species. |
format |
Other/Unknown Material |
author |
Zhou, Li Wang, Xueying Liu, Qinghua Yang, Jingkun Xu, Shihong Wu, Zhihao Wang, Yanfeng You, Feng Song, Zongcheng Li, Jun |
spellingShingle |
Zhou, Li Wang, Xueying Liu, Qinghua Yang, Jingkun Xu, Shihong Wu, Zhihao Wang, Yanfeng You, Feng Song, Zongcheng Li, Jun Successful spermatogonial stem cells transplantation within Pleuronectiformes: first breakthrough at inter-family level in marine fish |
author_facet |
Zhou, Li Wang, Xueying Liu, Qinghua Yang, Jingkun Xu, Shihong Wu, Zhihao Wang, Yanfeng You, Feng Song, Zongcheng Li, Jun |
author_sort |
Zhou, Li |
title |
Successful spermatogonial stem cells transplantation within Pleuronectiformes: first breakthrough at inter-family level in marine fish |
title_short |
Successful spermatogonial stem cells transplantation within Pleuronectiformes: first breakthrough at inter-family level in marine fish |
title_full |
Successful spermatogonial stem cells transplantation within Pleuronectiformes: first breakthrough at inter-family level in marine fish |
title_fullStr |
Successful spermatogonial stem cells transplantation within Pleuronectiformes: first breakthrough at inter-family level in marine fish |
title_full_unstemmed |
Successful spermatogonial stem cells transplantation within Pleuronectiformes: first breakthrough at inter-family level in marine fish |
title_sort |
successful spermatogonial stem cells transplantation within pleuronectiformes: first breakthrough at inter-family level in marine fish |
publisher |
Authorea, Inc. |
publishDate |
2024 |
url |
http://dx.doi.org/10.22541/au.170666192.24876538/v1 |
genre |
Turbot |
genre_facet |
Turbot |
op_doi |
https://doi.org/10.22541/au.170666192.24876538/v1 |
_version_ |
1800739679182520320 |