Exploring the Mechanisms of Anti‐Aβ42 Aggregation Activity of Walnut‐derived Peptides using Transcriptomics and Proteomics in vitro
Inhibiting β ‐amyloid (A β ) aggregation is of significance in finding potential candidates for Alzheimer's disease (AD) treatment. Accumulating evidence suggests that nutrition is important for improving cognition and reducing AD risk. Walnut has been widely used as a functional food for brain...
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crwiley:10.53365/efood.k/144885 2024-09-09T19:25:27+00:00 Exploring the Mechanisms of Anti‐Aβ42 Aggregation Activity of Walnut‐derived Peptides using Transcriptomics and Proteomics in vitro Wang, Min Gong, Congcong Amakye, William Kwame Ren, Jiaoyan China Postdoctoral Science Foundation 2021 http://dx.doi.org/10.53365/efood.k/144885 https://onlinelibrary.wiley.com/doi/pdf/10.53365/efood.k/144885 https://onlinelibrary.wiley.com/doi/full-xml/10.53365/efood.k/144885 en eng Wiley https://creativecommons.org/licenses/by/4.0/ eFood volume 2, issue 5, page 247-258 ISSN 2666-3066 2666-3066 journal-article 2021 crwiley https://doi.org/10.53365/efood.k/144885 2024-07-30T04:20:45Z Inhibiting β ‐amyloid (A β ) aggregation is of significance in finding potential candidates for Alzheimer's disease (AD) treatment. Accumulating evidence suggests that nutrition is important for improving cognition and reducing AD risk. Walnut has been widely used as a functional food for brain health; however the underlying mechanisms remain unknown. Here, we investigated the molecular level alteration in Arctic mutant A β 42 induced aggregation cell model by RNA‐seq and iTRAQ approaches after walnut‐derived peptides Pro‐Pro‐Lys‐Asn‐Trp (PW5) and Trp‐Pro‐Pro‐Lys‐Asn (WN5) interventions. PW5 or WN5 could significantly decrease abnormal A β 42 aggregates. However, resultant alterations in transcriptome (substantially unchanged) were inconsistent with proteomic data (marked change). Proteomic analysis revealed 184 and 194 differentially expressed proteins unique to PW5 and WN5 treatment, respectively, for inhibiting A β 42 protein production or increasing protein degradation via the mismatch repair pathways. Our study provides new insights into the effectiveness of food‐derived peptides for anti‐A β 42 aggregation in AD. Article in Journal/Newspaper Arctic Wiley Online Library Arctic eFood 2 5 247 258 |
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Wiley Online Library |
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English |
description |
Inhibiting β ‐amyloid (A β ) aggregation is of significance in finding potential candidates for Alzheimer's disease (AD) treatment. Accumulating evidence suggests that nutrition is important for improving cognition and reducing AD risk. Walnut has been widely used as a functional food for brain health; however the underlying mechanisms remain unknown. Here, we investigated the molecular level alteration in Arctic mutant A β 42 induced aggregation cell model by RNA‐seq and iTRAQ approaches after walnut‐derived peptides Pro‐Pro‐Lys‐Asn‐Trp (PW5) and Trp‐Pro‐Pro‐Lys‐Asn (WN5) interventions. PW5 or WN5 could significantly decrease abnormal A β 42 aggregates. However, resultant alterations in transcriptome (substantially unchanged) were inconsistent with proteomic data (marked change). Proteomic analysis revealed 184 and 194 differentially expressed proteins unique to PW5 and WN5 treatment, respectively, for inhibiting A β 42 protein production or increasing protein degradation via the mismatch repair pathways. Our study provides new insights into the effectiveness of food‐derived peptides for anti‐A β 42 aggregation in AD. |
author2 |
China Postdoctoral Science Foundation |
format |
Article in Journal/Newspaper |
author |
Wang, Min Gong, Congcong Amakye, William Kwame Ren, Jiaoyan |
spellingShingle |
Wang, Min Gong, Congcong Amakye, William Kwame Ren, Jiaoyan Exploring the Mechanisms of Anti‐Aβ42 Aggregation Activity of Walnut‐derived Peptides using Transcriptomics and Proteomics in vitro |
author_facet |
Wang, Min Gong, Congcong Amakye, William Kwame Ren, Jiaoyan |
author_sort |
Wang, Min |
title |
Exploring the Mechanisms of Anti‐Aβ42 Aggregation Activity of Walnut‐derived Peptides using Transcriptomics and Proteomics in vitro |
title_short |
Exploring the Mechanisms of Anti‐Aβ42 Aggregation Activity of Walnut‐derived Peptides using Transcriptomics and Proteomics in vitro |
title_full |
Exploring the Mechanisms of Anti‐Aβ42 Aggregation Activity of Walnut‐derived Peptides using Transcriptomics and Proteomics in vitro |
title_fullStr |
Exploring the Mechanisms of Anti‐Aβ42 Aggregation Activity of Walnut‐derived Peptides using Transcriptomics and Proteomics in vitro |
title_full_unstemmed |
Exploring the Mechanisms of Anti‐Aβ42 Aggregation Activity of Walnut‐derived Peptides using Transcriptomics and Proteomics in vitro |
title_sort |
exploring the mechanisms of anti‐aβ42 aggregation activity of walnut‐derived peptides using transcriptomics and proteomics in vitro |
publisher |
Wiley |
publishDate |
2021 |
url |
http://dx.doi.org/10.53365/efood.k/144885 https://onlinelibrary.wiley.com/doi/pdf/10.53365/efood.k/144885 https://onlinelibrary.wiley.com/doi/full-xml/10.53365/efood.k/144885 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
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Arctic |
op_source |
eFood volume 2, issue 5, page 247-258 ISSN 2666-3066 2666-3066 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.53365/efood.k/144885 |
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5 |
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258 |
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1809895246697332736 |