Extending the cell digestion assay to quantify dead phytoplankton cells in cold and polar waters
The cell digestion assay (CDA) is an effective method to quantify living/dead cells in complex natural phytoplankton communities. The CDA involves the incubation of fresh samples at 35°C for 45 min for the enzymatic digestion of dead cells. The relatively high incubation temperature has raised conce...
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crwiley:10.4319/lom.2008.6.659 2024-09-09T19:23:19+00:00 Extending the cell digestion assay to quantify dead phytoplankton cells in cold and polar waters Llabrés, Moira Agustí, Susana 2008 http://dx.doi.org/10.4319/lom.2008.6.659 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flom.2008.6.659 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lom.2008.6.659 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Limnology and Oceanography: Methods volume 6, issue 12, page 659-666 ISSN 1541-5856 1541-5856 journal-article 2008 crwiley https://doi.org/10.4319/lom.2008.6.659 2024-08-20T04:15:07Z The cell digestion assay (CDA) is an effective method to quantify living/dead cells in complex natural phytoplankton communities. The CDA involves the incubation of fresh samples at 35°C for 45 min for the enzymatic digestion of dead cells. The relatively high incubation temperature has raised concerns as to the applicability of the CDA to phytoplankton samples from polar and cold environments. Here, we examined the applicability of the CDA to phytoplankton growing at low temperatures and developed modifications and recommendations to reliably use the CDA in polar and cold‐water communities. We carried out different tests with polar and temperate phytoplankton species, and with natural communities from the Arctic Ocean. Increased cell death when applying the standard CDA method was evident in some of the species tested, when compared with results obtained using alternative staining membrane permeability tests. We confirmed that the use of an incubation temperature of 25°C with Arctic communities and cultured species growing at 5°C results in the complete digestion of dead cells and no significant effect on the percentage of living cells. We recommend the use of 25°C as the incubation temperature when applying the CDA to phytoplankton communities inhabiting cold environments. Article in Journal/Newspaper Arctic Arctic Ocean Phytoplankton Wiley Online Library Arctic Arctic Ocean Limnology and Oceanography: Methods 6 12 659 666 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
The cell digestion assay (CDA) is an effective method to quantify living/dead cells in complex natural phytoplankton communities. The CDA involves the incubation of fresh samples at 35°C for 45 min for the enzymatic digestion of dead cells. The relatively high incubation temperature has raised concerns as to the applicability of the CDA to phytoplankton samples from polar and cold environments. Here, we examined the applicability of the CDA to phytoplankton growing at low temperatures and developed modifications and recommendations to reliably use the CDA in polar and cold‐water communities. We carried out different tests with polar and temperate phytoplankton species, and with natural communities from the Arctic Ocean. Increased cell death when applying the standard CDA method was evident in some of the species tested, when compared with results obtained using alternative staining membrane permeability tests. We confirmed that the use of an incubation temperature of 25°C with Arctic communities and cultured species growing at 5°C results in the complete digestion of dead cells and no significant effect on the percentage of living cells. We recommend the use of 25°C as the incubation temperature when applying the CDA to phytoplankton communities inhabiting cold environments. |
format |
Article in Journal/Newspaper |
author |
Llabrés, Moira Agustí, Susana |
spellingShingle |
Llabrés, Moira Agustí, Susana Extending the cell digestion assay to quantify dead phytoplankton cells in cold and polar waters |
author_facet |
Llabrés, Moira Agustí, Susana |
author_sort |
Llabrés, Moira |
title |
Extending the cell digestion assay to quantify dead phytoplankton cells in cold and polar waters |
title_short |
Extending the cell digestion assay to quantify dead phytoplankton cells in cold and polar waters |
title_full |
Extending the cell digestion assay to quantify dead phytoplankton cells in cold and polar waters |
title_fullStr |
Extending the cell digestion assay to quantify dead phytoplankton cells in cold and polar waters |
title_full_unstemmed |
Extending the cell digestion assay to quantify dead phytoplankton cells in cold and polar waters |
title_sort |
extending the cell digestion assay to quantify dead phytoplankton cells in cold and polar waters |
publisher |
Wiley |
publishDate |
2008 |
url |
http://dx.doi.org/10.4319/lom.2008.6.659 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flom.2008.6.659 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lom.2008.6.659 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean Phytoplankton |
genre_facet |
Arctic Arctic Ocean Phytoplankton |
op_source |
Limnology and Oceanography: Methods volume 6, issue 12, page 659-666 ISSN 1541-5856 1541-5856 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.4319/lom.2008.6.659 |
container_title |
Limnology and Oceanography: Methods |
container_volume |
6 |
container_issue |
12 |
container_start_page |
659 |
op_container_end_page |
666 |
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1809763724441944064 |