Molecular detection of algal prey in copepod guts and fecal pellets
The ability to obtain information about feeding selectivity and rates in situ for key organisms such as copepods and other zooplankton is vital for understanding the mechanisms structuring marine ecosystems. Copepods feed on a wide range of prey, and there are presently no methods available to direc...
Published in: | Limnology and Oceanography: Methods |
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crwiley:10.4319/lom.2003.1.29 2024-06-23T07:51:54+00:00 Molecular detection of algal prey in copepod guts and fecal pellets Nejstgaard, Jens C. Frischer, Marc E. Raule, Caren L. Gruebel, Rita Kohlberg, Kathleen E. Verity, Peter G. 2003 http://dx.doi.org/10.4319/lom.2003.1.29 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flom.2003.1.29 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lom.2003.1.29 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Limnology and Oceanography: Methods volume 1, issue 1, page 29-38 ISSN 1541-5856 1541-5856 journal-article 2003 crwiley https://doi.org/10.4319/lom.2003.1.29 2024-05-31T08:14:34Z The ability to obtain information about feeding selectivity and rates in situ for key organisms such as copepods and other zooplankton is vital for understanding the mechanisms structuring marine ecosystems. Copepods feed on a wide range of prey, and there are presently no methods available to directly quantify zooplankton feeding on all different prey types in situ. Therefore, the development of a new nonintrusive direct method is necessary to gain a better understanding of the trophic interactions in aquatic ecosystems. Molecular methods based on the polymerase chain reaction have recently become an important tool to study predation by arthropods, particularly insects. Here we present the first results of successful molecular detection of a specific prey consumed by calanoid copepods from gut and fecal material. Using the calanoid copepod species Calanus finmarchicus consuming the haptophyte alga Emiliania huxleyi as a model system, 18S ribosomal DNA originating from E. huxleyi was unambiguously detected in whole DNA extracts from copepods and from their fecal pellets. The results also suggest that prey DNA may be quantified for determination of prey‐specific zooplankton feeding rates. However, significantly more research under controlled laboratory and field conditions will be required to achieve these objectives. We hypothesize that molecular methods will become an important tool with the potential to quantify undisturbed trophic interactions between individual predators and all their prey in the complex natural plankton. Article in Journal/Newspaper Calanus finmarchicus Copepods Wiley Online Library Limnology and Oceanography: Methods 1 1 29 38 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
The ability to obtain information about feeding selectivity and rates in situ for key organisms such as copepods and other zooplankton is vital for understanding the mechanisms structuring marine ecosystems. Copepods feed on a wide range of prey, and there are presently no methods available to directly quantify zooplankton feeding on all different prey types in situ. Therefore, the development of a new nonintrusive direct method is necessary to gain a better understanding of the trophic interactions in aquatic ecosystems. Molecular methods based on the polymerase chain reaction have recently become an important tool to study predation by arthropods, particularly insects. Here we present the first results of successful molecular detection of a specific prey consumed by calanoid copepods from gut and fecal material. Using the calanoid copepod species Calanus finmarchicus consuming the haptophyte alga Emiliania huxleyi as a model system, 18S ribosomal DNA originating from E. huxleyi was unambiguously detected in whole DNA extracts from copepods and from their fecal pellets. The results also suggest that prey DNA may be quantified for determination of prey‐specific zooplankton feeding rates. However, significantly more research under controlled laboratory and field conditions will be required to achieve these objectives. We hypothesize that molecular methods will become an important tool with the potential to quantify undisturbed trophic interactions between individual predators and all their prey in the complex natural plankton. |
format |
Article in Journal/Newspaper |
author |
Nejstgaard, Jens C. Frischer, Marc E. Raule, Caren L. Gruebel, Rita Kohlberg, Kathleen E. Verity, Peter G. |
spellingShingle |
Nejstgaard, Jens C. Frischer, Marc E. Raule, Caren L. Gruebel, Rita Kohlberg, Kathleen E. Verity, Peter G. Molecular detection of algal prey in copepod guts and fecal pellets |
author_facet |
Nejstgaard, Jens C. Frischer, Marc E. Raule, Caren L. Gruebel, Rita Kohlberg, Kathleen E. Verity, Peter G. |
author_sort |
Nejstgaard, Jens C. |
title |
Molecular detection of algal prey in copepod guts and fecal pellets |
title_short |
Molecular detection of algal prey in copepod guts and fecal pellets |
title_full |
Molecular detection of algal prey in copepod guts and fecal pellets |
title_fullStr |
Molecular detection of algal prey in copepod guts and fecal pellets |
title_full_unstemmed |
Molecular detection of algal prey in copepod guts and fecal pellets |
title_sort |
molecular detection of algal prey in copepod guts and fecal pellets |
publisher |
Wiley |
publishDate |
2003 |
url |
http://dx.doi.org/10.4319/lom.2003.1.29 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flom.2003.1.29 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lom.2003.1.29 |
genre |
Calanus finmarchicus Copepods |
genre_facet |
Calanus finmarchicus Copepods |
op_source |
Limnology and Oceanography: Methods volume 1, issue 1, page 29-38 ISSN 1541-5856 1541-5856 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.4319/lom.2003.1.29 |
container_title |
Limnology and Oceanography: Methods |
container_volume |
1 |
container_issue |
1 |
container_start_page |
29 |
op_container_end_page |
38 |
_version_ |
1802643047222083584 |