Distinguishing between the effects of ocean acidification and ocean carbonation in the coccolithophore Emiliania huxleyi

• Published in: Limnology and Oceanography
• Main Authors: Bach, Lennart Thomas, Riebesell, Ulf, Schulz, Kai Georg
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2011
• Subjects: Ocean acidification
• Online Access: http://dx.doi.org/10.4319/lo.2011.56.6.2040; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.2011.56.6.2040; https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.2011.56.6.2040

The coccolithophore Emiliania huxleyi was cultured under a broad range of carbonate chemistry conditions to distinguish the effects of individual carbonate system parameters on growth, primary production, and calcification. In the first experiment, alkalinity was kept constant and the fugacity of CO 2 (fCO 2 ) varied from 2 to 600 Pa (1 Pa ≈ 10 µatm). In the second experiment, pH was kept constant (pH free = 8) with fCO 2 varying from 4 to 370 Pa. Results of the constant‐alkalinity approach revealed physiological optima for growth, calcification, and organic carbon production at fCO 2 values of ~ 20 Pa, ~ 40 Pa, and ~ 80 Pa, respectively. Comparing this with the constant‐pH approach showed that growth and organic carbon production increased similarly from low to intermediate CO 2 levels but started to diverge towards higher CO 2 levels. In the high CO 2 range, growth rates and organic carbon production decreased steadily with declining pH at constant alkalinity while remaining consistently higher at constant pH. This suggests that growth and organic carbon production rates are directly related to CO 2 at low (sub‐saturating) concentrations, whereas towards higher CO 2 levels they are adversely affected by the associated decrease in pH. A pH dependence at high fCO 2 is also indicated for calcification rates, while the key carbonate system parameter determining calcification at low fCO 2 remains unclear. These results imply that key metabolic processes in coccolithophores have their optima at different carbonate chemistry conditions and are influenced by different parameters of the carbonate system at both sides of the optimum.