Assessment of relative phosphorus limitation of Trichodesmium spp. in the North Pacific, North Atlantic, and the north coast of Australia
Trichodesmium spp. is a colonial diazotrophic cyanobacterium that occurs in the oligotrophic tropics and subtropics. Because of its ability to fix atmospheric N 2 , it is likely to be growth limited by P or Fe, and it has been hypothesized that limitation differs among different ocean basins. Two as...
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crwiley:10.4319/lo.2008.53.6.2495 2024-06-23T07:54:54+00:00 Assessment of relative phosphorus limitation of Trichodesmium spp. in the North Pacific, North Atlantic, and the north coast of Australia Sohm, Jill A. Mahaffey, Claire Capone, Douglas G. 2008 http://dx.doi.org/10.4319/lo.2008.53.6.2495 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.2008.53.6.2495 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.2008.53.6.2495 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Limnology and Oceanography volume 53, issue 6, page 2495-2502 ISSN 0024-3590 1939-5590 journal-article 2008 crwiley https://doi.org/10.4319/lo.2008.53.6.2495 2024-06-11T04:43:39Z Trichodesmium spp. is a colonial diazotrophic cyanobacterium that occurs in the oligotrophic tropics and subtropics. Because of its ability to fix atmospheric N 2 , it is likely to be growth limited by P or Fe, and it has been hypothesized that limitation differs among different ocean basins. Two assays used as indices of P limitation or stress in Trichodesmium spp. are uptake of 33 PO 4 3‐ to determine maximal P uptake ( V max ) and hydrolysis of P from methylumbelliferone phosphate to estimate alkaline phosphatase activity (APA). The kinetics of PO 4 3‐ uptake were determined for Trichodesmium spp. colonies in the North Pacific, North Atlantic, and in waters north of Australia, whereas APA was determined in the North Pacific and North Atlantic. Trichodesmium spp. V max was significantly greater (~fourfold or more) in the North Atlantic compared with the North Pacific and waters north of Australia when normalized to both chlorophyll a content and number of trichomes per colony. APA in the North Atlantic was also greater than in the North Pacific. The half‐saturation constant for PO 4 3‐ uptake ( K s ) was not significantly different among the three locations. These data indicate that Trichodesmium spp. is more strongly P limited in the North Atlantic compared with the North Pacific or waters along the north coast of Australia. We suggest that the Trichodesmium spp. communities in the North Pacific and waters north of Australia are primarily Fe rather than P stressed and that these differences reflect differing relative inputs and availability of two major controlling variables for diazotrophy, P and Fe, in these geographically divergent areas. Article in Journal/Newspaper North Atlantic Wiley Online Library Pacific Limnology and Oceanography 53 6 2495 2502 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Trichodesmium spp. is a colonial diazotrophic cyanobacterium that occurs in the oligotrophic tropics and subtropics. Because of its ability to fix atmospheric N 2 , it is likely to be growth limited by P or Fe, and it has been hypothesized that limitation differs among different ocean basins. Two assays used as indices of P limitation or stress in Trichodesmium spp. are uptake of 33 PO 4 3‐ to determine maximal P uptake ( V max ) and hydrolysis of P from methylumbelliferone phosphate to estimate alkaline phosphatase activity (APA). The kinetics of PO 4 3‐ uptake were determined for Trichodesmium spp. colonies in the North Pacific, North Atlantic, and in waters north of Australia, whereas APA was determined in the North Pacific and North Atlantic. Trichodesmium spp. V max was significantly greater (~fourfold or more) in the North Atlantic compared with the North Pacific and waters north of Australia when normalized to both chlorophyll a content and number of trichomes per colony. APA in the North Atlantic was also greater than in the North Pacific. The half‐saturation constant for PO 4 3‐ uptake ( K s ) was not significantly different among the three locations. These data indicate that Trichodesmium spp. is more strongly P limited in the North Atlantic compared with the North Pacific or waters along the north coast of Australia. We suggest that the Trichodesmium spp. communities in the North Pacific and waters north of Australia are primarily Fe rather than P stressed and that these differences reflect differing relative inputs and availability of two major controlling variables for diazotrophy, P and Fe, in these geographically divergent areas. |
format |
Article in Journal/Newspaper |
author |
Sohm, Jill A. Mahaffey, Claire Capone, Douglas G. |
spellingShingle |
Sohm, Jill A. Mahaffey, Claire Capone, Douglas G. Assessment of relative phosphorus limitation of Trichodesmium spp. in the North Pacific, North Atlantic, and the north coast of Australia |
author_facet |
Sohm, Jill A. Mahaffey, Claire Capone, Douglas G. |
author_sort |
Sohm, Jill A. |
title |
Assessment of relative phosphorus limitation of Trichodesmium spp. in the North Pacific, North Atlantic, and the north coast of Australia |
title_short |
Assessment of relative phosphorus limitation of Trichodesmium spp. in the North Pacific, North Atlantic, and the north coast of Australia |
title_full |
Assessment of relative phosphorus limitation of Trichodesmium spp. in the North Pacific, North Atlantic, and the north coast of Australia |
title_fullStr |
Assessment of relative phosphorus limitation of Trichodesmium spp. in the North Pacific, North Atlantic, and the north coast of Australia |
title_full_unstemmed |
Assessment of relative phosphorus limitation of Trichodesmium spp. in the North Pacific, North Atlantic, and the north coast of Australia |
title_sort |
assessment of relative phosphorus limitation of trichodesmium spp. in the north pacific, north atlantic, and the north coast of australia |
publisher |
Wiley |
publishDate |
2008 |
url |
http://dx.doi.org/10.4319/lo.2008.53.6.2495 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.2008.53.6.2495 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.2008.53.6.2495 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Limnology and Oceanography volume 53, issue 6, page 2495-2502 ISSN 0024-3590 1939-5590 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.4319/lo.2008.53.6.2495 |
container_title |
Limnology and Oceanography |
container_volume |
53 |
container_issue |
6 |
container_start_page |
2495 |
op_container_end_page |
2502 |
_version_ |
1802647234017230848 |