Standing stocks and activity of Archaea and Bacteria in the western Arctic Ocean
This study examined the abundance, cell size, and activity of Bacteria and Archaea in the Chukchi Sea and the Canada Basin of the western Arctic Ocean in the spring (May—June) and summer (July—August) of 2002 and 2004. Data from fluorescence in situ hybridization (FISH) analyses indicate that bacter...
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crwiley:10.4319/lo.2007.52.2.0495 2024-09-15T17:53:31+00:00 Standing stocks and activity of Archaea and Bacteria in the western Arctic Ocean Kirchman, David L. Elifantz, Hila Dittel, Ana I. Malmstrom, Rex R. Cottrell, Matthew T. 2007 http://dx.doi.org/10.4319/lo.2007.52.2.0495 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.2007.52.2.0495 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.2007.52.2.0495 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Limnology and Oceanography volume 52, issue 2, page 495-507 ISSN 0024-3590 1939-5590 journal-article 2007 crwiley https://doi.org/10.4319/lo.2007.52.2.0495 2024-08-01T04:23:54Z This study examined the abundance, cell size, and activity of Bacteria and Archaea in the Chukchi Sea and the Canada Basin of the western Arctic Ocean in the spring (May—June) and summer (July—August) of 2002 and 2004. Data from fluorescence in situ hybridization (FISH) analyses indicate that bacterial abundance as a percent of total prokaryotes decreased with depth, whereas in contrast, Crenarchaeota increased from about 10%of prokaryotes in surface waters to as much as 40% in samples from 100 to 200 m. Euryarchaeota were detectable in only a few samples. Relative abundance of Crenarchaeota, expressed as a percent of total prokaryotes, correlated with ammonium concentrations, but relative bacterial abundance did not. Crenarchaeota cells were significantly larger than Bacteria by 1.5‐ to 2‐fold in the upper 200 m. Data collected from a combination of FISH and microautoradiography indicate that often the fraction of both Bacteria and Crenarchaeota assimilating organic compounds was high (up to 55%), and both microbial groups were more active in assimilating amino acids than other compounds. However, Crenarchaeota were usually less active than Bacteria in assimilating amino acids and glucose, but were nearly as active as Bacteria in assimilating protein and diatom extracellular polymers. The fraction of Bacteria and Crenarchaeota assimilating CO 2 in surface waters was higher than expected by anaplerotic fixation alone, suggesting that many of these microbes are chemoautotrophic. These data add to a growing body of evidence indicating how the roles of Archaea and Bacteria differ in biogeochemical cycles of the oceans. Article in Journal/Newspaper Arctic Ocean canada basin Chukchi Chukchi Sea Wiley Online Library Limnology and Oceanography 52 2 495 507 |
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English |
description |
This study examined the abundance, cell size, and activity of Bacteria and Archaea in the Chukchi Sea and the Canada Basin of the western Arctic Ocean in the spring (May—June) and summer (July—August) of 2002 and 2004. Data from fluorescence in situ hybridization (FISH) analyses indicate that bacterial abundance as a percent of total prokaryotes decreased with depth, whereas in contrast, Crenarchaeota increased from about 10%of prokaryotes in surface waters to as much as 40% in samples from 100 to 200 m. Euryarchaeota were detectable in only a few samples. Relative abundance of Crenarchaeota, expressed as a percent of total prokaryotes, correlated with ammonium concentrations, but relative bacterial abundance did not. Crenarchaeota cells were significantly larger than Bacteria by 1.5‐ to 2‐fold in the upper 200 m. Data collected from a combination of FISH and microautoradiography indicate that often the fraction of both Bacteria and Crenarchaeota assimilating organic compounds was high (up to 55%), and both microbial groups were more active in assimilating amino acids than other compounds. However, Crenarchaeota were usually less active than Bacteria in assimilating amino acids and glucose, but were nearly as active as Bacteria in assimilating protein and diatom extracellular polymers. The fraction of Bacteria and Crenarchaeota assimilating CO 2 in surface waters was higher than expected by anaplerotic fixation alone, suggesting that many of these microbes are chemoautotrophic. These data add to a growing body of evidence indicating how the roles of Archaea and Bacteria differ in biogeochemical cycles of the oceans. |
format |
Article in Journal/Newspaper |
author |
Kirchman, David L. Elifantz, Hila Dittel, Ana I. Malmstrom, Rex R. Cottrell, Matthew T. |
spellingShingle |
Kirchman, David L. Elifantz, Hila Dittel, Ana I. Malmstrom, Rex R. Cottrell, Matthew T. Standing stocks and activity of Archaea and Bacteria in the western Arctic Ocean |
author_facet |
Kirchman, David L. Elifantz, Hila Dittel, Ana I. Malmstrom, Rex R. Cottrell, Matthew T. |
author_sort |
Kirchman, David L. |
title |
Standing stocks and activity of Archaea and Bacteria in the western Arctic Ocean |
title_short |
Standing stocks and activity of Archaea and Bacteria in the western Arctic Ocean |
title_full |
Standing stocks and activity of Archaea and Bacteria in the western Arctic Ocean |
title_fullStr |
Standing stocks and activity of Archaea and Bacteria in the western Arctic Ocean |
title_full_unstemmed |
Standing stocks and activity of Archaea and Bacteria in the western Arctic Ocean |
title_sort |
standing stocks and activity of archaea and bacteria in the western arctic ocean |
publisher |
Wiley |
publishDate |
2007 |
url |
http://dx.doi.org/10.4319/lo.2007.52.2.0495 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.2007.52.2.0495 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.2007.52.2.0495 |
genre |
Arctic Ocean canada basin Chukchi Chukchi Sea |
genre_facet |
Arctic Ocean canada basin Chukchi Chukchi Sea |
op_source |
Limnology and Oceanography volume 52, issue 2, page 495-507 ISSN 0024-3590 1939-5590 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.4319/lo.2007.52.2.0495 |
container_title |
Limnology and Oceanography |
container_volume |
52 |
container_issue |
2 |
container_start_page |
495 |
op_container_end_page |
507 |
_version_ |
1810429391805612032 |