Cell‐specific detection of phosphorus stress in Trichodesmium from the Western North Atlantic
The low phosphorus concentrations observed in the western North Atlantic and the western Central Atlantic suggest that phosphorus bioavailability may limit Trichodesmium productivity and N 2 fixation. However, the degree to which the concentration and composition of the total phosphorus pool affects...
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Online Access: | http://dx.doi.org/10.4319/lo.2002.47.6.1832 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.2002.47.6.1832 https://onlinelibrary.wiley.com/doi/pdf/10.4319/lo.2002.47.6.1832 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.2002.47.6.1832 |
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crwiley:10.4319/lo.2002.47.6.1832 2024-09-15T18:21:35+00:00 Cell‐specific detection of phosphorus stress in Trichodesmium from the Western North Atlantic Dyhrman, Sonya T. Webb, Eric A. Anderson, Donald M. Moffett, James W. Waterbury, John B. 2002 http://dx.doi.org/10.4319/lo.2002.47.6.1832 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.2002.47.6.1832 https://onlinelibrary.wiley.com/doi/pdf/10.4319/lo.2002.47.6.1832 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.2002.47.6.1832 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Limnology and Oceanography volume 47, issue 6, page 1832-1836 ISSN 0024-3590 1939-5590 journal-article 2002 crwiley https://doi.org/10.4319/lo.2002.47.6.1832 2024-07-23T04:12:55Z The low phosphorus concentrations observed in the western North Atlantic and the western Central Atlantic suggest that phosphorus bioavailability may limit Trichodesmium productivity and N 2 fixation. However, the degree to which the concentration and composition of the total phosphorus pool affects actual bioavailability is poorly understood. To better examine how phosphorus bioavailability may constrain primary production and marine N 2 fixation, we have developed a molecular diagnostic tool for quickly detecting the phosphorus status of the diazotroph Trichodesmium . This diagnostic method uses a commercially available substrate to fluorescently tag cells expressing the phosphate‐regulated enzyme, alkaline phosphatase. Using this diagnostic tool, we were able to distinguish phosphorus‐replete from phosphorus‐stressed Trichodesmium populations along a transect from Grand Bahama Island to Bermuda in November 2000. Article in Journal/Newspaper North Atlantic Wiley Online Library Limnology and Oceanography 47 6 1832 1836 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
The low phosphorus concentrations observed in the western North Atlantic and the western Central Atlantic suggest that phosphorus bioavailability may limit Trichodesmium productivity and N 2 fixation. However, the degree to which the concentration and composition of the total phosphorus pool affects actual bioavailability is poorly understood. To better examine how phosphorus bioavailability may constrain primary production and marine N 2 fixation, we have developed a molecular diagnostic tool for quickly detecting the phosphorus status of the diazotroph Trichodesmium . This diagnostic method uses a commercially available substrate to fluorescently tag cells expressing the phosphate‐regulated enzyme, alkaline phosphatase. Using this diagnostic tool, we were able to distinguish phosphorus‐replete from phosphorus‐stressed Trichodesmium populations along a transect from Grand Bahama Island to Bermuda in November 2000. |
format |
Article in Journal/Newspaper |
author |
Dyhrman, Sonya T. Webb, Eric A. Anderson, Donald M. Moffett, James W. Waterbury, John B. |
spellingShingle |
Dyhrman, Sonya T. Webb, Eric A. Anderson, Donald M. Moffett, James W. Waterbury, John B. Cell‐specific detection of phosphorus stress in Trichodesmium from the Western North Atlantic |
author_facet |
Dyhrman, Sonya T. Webb, Eric A. Anderson, Donald M. Moffett, James W. Waterbury, John B. |
author_sort |
Dyhrman, Sonya T. |
title |
Cell‐specific detection of phosphorus stress in Trichodesmium from the Western North Atlantic |
title_short |
Cell‐specific detection of phosphorus stress in Trichodesmium from the Western North Atlantic |
title_full |
Cell‐specific detection of phosphorus stress in Trichodesmium from the Western North Atlantic |
title_fullStr |
Cell‐specific detection of phosphorus stress in Trichodesmium from the Western North Atlantic |
title_full_unstemmed |
Cell‐specific detection of phosphorus stress in Trichodesmium from the Western North Atlantic |
title_sort |
cell‐specific detection of phosphorus stress in trichodesmium from the western north atlantic |
publisher |
Wiley |
publishDate |
2002 |
url |
http://dx.doi.org/10.4319/lo.2002.47.6.1832 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.4319%2Flo.2002.47.6.1832 https://onlinelibrary.wiley.com/doi/pdf/10.4319/lo.2002.47.6.1832 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.4319/lo.2002.47.6.1832 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Limnology and Oceanography volume 47, issue 6, page 1832-1836 ISSN 0024-3590 1939-5590 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.4319/lo.2002.47.6.1832 |
container_title |
Limnology and Oceanography |
container_volume |
47 |
container_issue |
6 |
container_start_page |
1832 |
op_container_end_page |
1836 |
_version_ |
1810460227195109376 |